Effects of Polyamine Antimetabolites on the Growth Rate of Human Prostatic Tumors Transplantable into Nude Mice

2015 ◽  
pp. 370-373 ◽  
Author(s):  
Johan C. Romijn ◽  
Gert-Jan van Steenbrugge ◽  
Fritz H. Schr�der
Keyword(s):  
Growth Rate ◽  
Nude Mice

Lentiviral-Mediated Beclin-1 Overexpression Inhibits Cell Proliferation and Induces Autophagy of Human Esophageal Carcinoma Eca109 Cell Xenograft in Nude Mice

2020 ◽  
Vol 15 (1) ◽  
pp. 70-77
Author(s):  
Junhe Zhang ◽  
Weihua Dong
Keyword(s):  
Growth Rate ◽  
Cell Proliferation ◽  
Esophageal Carcinoma ◽  
Nude Mice ◽  
Beclin 1 ◽  
Control Group ◽  
Vector Group ◽  
Empty Vector ◽  
Empty Vector Group

Background: Esophageal carcinoma is one of the common malignant tumors in digestive tract. BECLIN-1 is a key gene that regulates autophagy, and its abnormal expression may be related with many human tumors. However, the mechanism of BECLIN-1 in esophageal carcinoma remains unknown. Objective: In this study, we explored the effect of BECLIN-1 overexpression on tumor growth in mice with esophageal carcinoma and its mechanism. Methods: Recombined lentiviral vector containing BECLIN-1 was used to transfect human esophageal carcinoma Eca109 cells and establish stable cell line. qRT-PCR was used to detect BECLIN-1 mRNA level in the transfected Eca109 cells, CCK-8 assay was used to detect cell proliferation. Beclin-1, P62 and LC3-II protein expression levels in Eca109 cells were detected using Western blot analysis. Subcutaneous xenograft nude mice model of human esophageal carcinoma was established, and the tumor growths in Beclin-1 group, control group and empty vector group were monitored. Beclin-1 protein expression in vivo was detected by immunohistochemistry. Results: Beclin-1 mRNA and protein were overexpressed in Eca109 cells. Compared with empty vector group, the growth rate of cells transfected with BECLIN-1 decreased significantly. Compared with the control group and empty vector group, the expression level of P62 protein in beclin-1 group was significantly decreased, while the expression level of LC3-II protein was significantly increased. The tumor growth rate in nude mice of Beclin-1 group was significantly lower than that of the control group and empty vector group, and Beclin-1 protein was mainly expressed in Beclin-1 group in vivo. Conclusion: BECLIN-1 can induce autophagy in esophageal carcinoma Eca109 cells, and it can significantly inhibit the growth of esophageal carcinoma.

A nanoemulsion of an anti-oxidant synergy formulation reduces tumor growth rate in neuroblastoma-bearing nude mice

2007 ◽  
Vol 66 (5) ◽  
pp. 428
Author(s):  
Fonghsu Kuo ◽  
Timothy Kotyla ◽  
Thomas Wilson ◽  
Lydia Kifle ◽  
Thomai Panagiotou ◽  
...  
Keyword(s):  
Growth Rate ◽  
Tumor Growth ◽  
Nude Mice ◽  
Tumor Growth Rate ◽  
Anti Oxidant

scholarly journals A nanoemulsion of an anti‐oxidant synergy formulation reduces tumor growth rate in neuroblastoma‐bearing nude mice.

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Fonghsu Kuo ◽  
Timothy Kotyla ◽  
Thomas Wilson ◽  
Lydia Kifle ◽  
Thomai Panagiotou ◽  
...  
Keyword(s):  
Growth Rate ◽  
Tumor Growth ◽  
Nude Mice ◽  
Tumor Growth Rate ◽  
Anti Oxidant

Effect of Carboxylesterase Inhibition on the Anti-Tumour Effects of Irinotecan

2005 ◽  
Vol 33 (1) ◽  
pp. 84-89 ◽  
Author(s):  
Y Morishita ◽  
M Fujii ◽  
Y Kasakura ◽  
T Takayama
Keyword(s):  
Growth Rate ◽  
Adverse Effects ◽  
Nude Mice ◽  
Anticancer Agent ◽  
Specific Inhibitor ◽  
Optimal Dose ◽  
Optimum Dose ◽  
The Relationship ◽  
Opening Up ◽  
Individualized Chemotherapy

Irinotecan (CPT-11) is an important anticancer agent activated by carboxylesterase (CE). Treatment with CPT-11 may be associated with severe adverse effects, however, so determining the optimal dose would greatly benefit patients. We investigated the relationship between the anti-tumour effects of CPT-11 and CE concentration using bis- p-nitrophenylphosphate (BNPP), a specific inhibitor of CE, in nude mice with xenograft tumours. Initial experiments showed that the optimal dose of CPT-11 was 100 mg/kg. This dose was then used to study the anti-tumour effects of CPT-11 with and without BNPP. A direct correlation was found between the dose of administered BNPP and the growth rate of the tumour, demonstrating that the anti-tumour effects of CPT-11 were related to the CE concentration. Measuring the concentration of CE may allow the optimum dose of CPT-11 to be determined, opening up the possibility of individualized chemotherapy programmes.

scholarly journals In Vivo Antitumor Effect of Supercritical CO2 Extract of Mango Ginger (Curcuma amada Roxb) in U-87MG Human Glioblastoma Nude Mice Xenografts

2016 ◽  
Vol 22 (2) ◽  
pp. 260-267 ◽  
Author(s):  
Cheppail Ramachandran ◽  
Gilda M. Portalatin ◽  
Adriana M. Prado ◽  
Karl-Werner Quirin ◽  
Enrique Escalon ◽  
...  
Keyword(s):  
Growth Rate ◽  
Glioblastoma Multiforme ◽  
Tumor Growth ◽  
Nude Mice ◽  
Antitumor Effect ◽  
Tumor Growth Rate ◽  
Human Glioblastoma ◽  
Mango Ginger ◽  
Curcuma Amada

Glioblastoma multiforme (GBM) is one the most aggressive and lethal human neoplasms with poor prognosis and very limited positive treatment options. The antitumor effect of supercritical CO2 extract of mango ginger ( Curcuma amada Roxb) (CA) with and without irinotecan (IR) was analyzed in U-87MG human glioblastoma multiforme (GBM) cells in vitro and in nude mice xenografts. CA is highly cytotoxic to GBM cells and is synergistic with IR as indicated by the combination index values of <1 in the CompuSyn analysis. CA inhibits tumor growth rate in GBM xenografts, the inhibition rate being higher than in IR treated group. GBM xenograft mice treated with IR + CA combination showed almost complete inhibition of tumor growth rate. Gene expression analysis of xenograft tumors indicated that IR + CA treatment significantly downregulated anti-apoptotic (Bcl-2 and mutant p53), inflammation-associated (COX-2) and cell division–associated (CCNB2) genes and upregulated pro-apoptotic genes (p21 and caspase-3). These results confirmed the therapeutic efficiency of IR + CA combination against GBM and the need for further clinical investigations.

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