Is there Functional Significance for Dopamine Antagonist Binding Sites on Lymphoid Cells?

2015 ◽  
pp. 123-141 ◽  
Author(s):  
E. G. Shaskan ◽  
M. Ballow ◽  
L. Oreland ◽  
G. Wadell
Keyword(s):  
Binding Sites ◽  
Functional Significance ◽  
Lymphoid Cells ◽  
Dopamine Antagonist ◽  
Antagonist Binding

Spiroperidol binding sites on mouse lymphoid cells

1984 ◽  
Vol 6 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Edward G. Shaskan ◽  
Mark Ballow ◽  
Marc Lederman ◽  
Sandra L. Margoles ◽  
Richard Melchreit
Keyword(s):  
Binding Sites ◽  
Lymphoid Cells

The Regulation and Functional Significance of Fc Receptors on Murine Lymphoid Cells: The Special Case of T Cells

1993 ◽  
pp. 443-449
Author(s):  
R. G. Lynch ◽  
M. Sandor ◽  
R. Nunez ◽  
M. Hagen ◽  
P. Teeraratkul ◽  
...  
Keyword(s):  
T Cells ◽  
Functional Significance ◽  
Fc Receptors ◽  
Lymphoid Cells ◽  
Special Case

scholarly journals Differential functional significance of AP-1 binding sites in the promoter of the gene encoding mouse tissue inhibitor of metalloproteinases-3

1997 ◽  
Vol 324 (2) ◽  
pp. 547-553 ◽  
Author(s):  
Hyungtae KIM ◽  
William D. PENNIE ◽  
Yi SUN ◽  
Nancy H. COLBURN
Keyword(s):  
Binding Site ◽  
Binding Sites ◽  
Functional Significance ◽  
Regulatory Elements ◽  
Binding Activity ◽  
Model Systems ◽  
Mouse Tissue ◽  
Significant Activity ◽  
Tissue Inhibitor Of Metalloproteinases ◽  
Tissue Inhibitor

Tissue inhibitor of metalloproteinases-3 (TIMP-3) is an extracellular-matrix-associated protein that suppresses tumorigenicity or invasion in several model systems. We have identified, by in vitro footprinting, six AP-1 (activator protein-1) or AP-1-like binding sites in the mouse TIMP-3 promoter that bind purified c-Jun homodimers. Electrophoretic mobility shift assays revealed that the non-consensus fifth AP-1 binding site (AP-720; nt -720 to -714) had the strongest binding activity for recombinant c-Jun protein, and that the fourth binding site (AP-763; nt -763 to -754) and AP-720 showed strong binding activity for cellular nuclear proteins. Antibody supershift and blocking experiments suggest that AP-720, but not AP-763, binds authentic AP-1 components. Transient transfection reporter assays of deletion constructs showed that the region spanning AP-720 has the highest transcriptional activity, and that sequences 5′ to this region (nt -2846 to -747) may contain negative regulatory elements. The deletion construct containing about 500 nt 5′ to the transcriptional start, but no AP-1 sites, showed lower but significant activity, suggesting both AP-1-dependent and -independent regulation of the mouse TIMP-3 promoter. Mutational inactivation of AP-720 abolished the activity increment that distinguished the reporter construct containing both AP-720 and sixth AP-1 binding site (AP-617; nt -617 to -611) from that containing only AP-617. In summary, we report here that both AP-1 and non-AP-1 elements contribute to activity, with the non-consensus AP-1 site at -720 showing the greatest functional significance among the AP-1 sites.

The interaction of BBR 2160 with calcium channel antagonist binding sites

1990 ◽  
Vol 22 ◽  
pp. 97
Author(s):  
I. Casciarri ◽  
R. Ceserani ◽  
M. Germini ◽  
D. Barone ◽  
F. Di Giovanni
Keyword(s):  
Calcium Channel ◽  
Calcium Channel Antagonist ◽  
Binding Sites ◽  
Antagonist Binding
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