Diagnostic Significance of Standardized Determination of Urinary Enzymes1 in the Practice of Human Kidney Transplantation and Renal Toxicology

2015 ◽  
pp. 105-127 ◽  
Author(s):  
Geza Horpácsy
Keyword(s):  
Kidney Transplantation ◽  
Human Kidney ◽  
Diagnostic Significance

scholarly journals PRO – AND ANTI-INFLAMMATORY CYTOKINES ARE EARLY MARKERS OF ACUTE REJECTION OF THE TRANSPLANTED KIDNEY

2018 ◽  
Vol 25 (3) ◽  
pp. 119-124
Author(s):  
T. V. FEDORENKO ◽  
N. V. KOLESNIKOVA ◽  
E. F. FILIPPOV
Keyword(s):  
Renal Failure ◽  
Kidney Transplantation ◽  
Acute Rejection ◽  
Inflammatory Cytokines ◽  
Post Transplantation ◽  
Diagnostic Significance ◽  
Hla Antibodies ◽  
Anti Inflammatory ◽  
Xmap Technology

Aim. Determination of diagnostic significance of pro − and anti-inflammatory cytokines in early prognosis of posttransplant renal failure in patients with chronic renal disease.Materials and methods. In the peripheral blood of patients with chronic kidney disease 6 hours before kidney transplantation, multiplex analysis using Simplex ProcartaPlex panel (Bioscience, USA) and xMAP technology (principle of flow cytometry) was used to estimate the content of 10 cytokines: 7 proinflammatory (IL-1b, IL-6, IL-12p70, IL-27, IL-17A, IL-18) and 3 antiinflammatory (IL-1RA, IL-4, IL-13). Identification of HLA-antibodies was carried out with the help of multiplex immunological analysis, using test systems (Gen-Prob, USA), flow analyzer Luminex 200 xMAP technology (bimolecular reactions on the surface of microspheres).Results. The limited diagnostic significance of HLA-antibodies is due to the fact that their detection in the posttransplantation period can be either in the development of acute graft rejection, or in the favorable course of the period after the operation. Meanwhile, the determination of a number of blood cytokines before kidney transplantation allows predicting post-transplantation rejection. In particular, certain criteria favorable course of the period after kidney transplantation by absence of HLA antibodies in patients with chronic renal failure can be considered as the initial (within 6 hours of transplantation) low levels of IL1β, IL6, IL17а. Prognostically the increase in the blood levels of proinflammatory cytokines − IL6, IL17a and anti-inflammatory IL1-RA is a significant marker of acute rejection of a transplanted kidney. Along with this, it is important to note that the appearance of HLA antibodies in patients with a favorable course of the post-transplantation period is associated with an initially elevated level of proinflammatory cytokines such as Il1ß and IL6.Conclusion. Diagnostic value of the evaluated cytokines at the pre-transplant kidney patients determines the feasibility of the inclusion of evaluation of the serum concentration of IL1β, IL6, IL17a, IL1-RA in the programme of pre-transplant laboratory tests .

scholarly journals Immunogenetic Predictors of Severe COVID-19

Vaccines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 211
Author(s):  
Anna Malkova ◽  
Dmitriy Kudlay ◽  
Igor Kudryavtsev ◽  
Anna Starshinova ◽  
Piotr Yablonskiy ◽  
...  
Keyword(s):  
Genetic Factors ◽  
Virus Disease ◽  
Analysis Data ◽  
Published Data ◽  
Diagnostic Significance ◽  
Life Threatening ◽  
Coronavirus Infection ◽  
Angiotension Converting Enzyme ◽  
Related Proteins

According to an analysis of published data, only 20% of patients with the new coronavirus infection develop severe life-threatening complications. Currently, there are no known biomarkers, the determination of which before the onset of the disease would allow assessing the likelihood of its severe course. The purpose of this literature review was to analyze possible genetic factors characterizing the immune response to the new coronavirus infection that could be associated with the expression of angiotension-converting enzyme 2 (ACE-2) and related proteins as predictors of severe Corona virus disease 2019 (COVID-19). We analyzed original articles published in Medline, PubMed and Scopus databases from December 2019 to November 2020. For searching articles, we used the following keywords: New coronavirus infection, Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), COVID-19, severe course, complications, thrombosis, cytokine storm, ACE-2, biomarkers. In total, 3714 publications were selected using the keywords, of which 8 were in congruence with all the criteria. The literature analysis of the association of immunogenic characteristics and the expression of ACE-2 and related proteins with the development of severe COVID-19 revealed following genetic factors: HLA-B*46:01 genotype, CXCR6 gene hypoexpression, CCR9 gene expression, TLR7, rs150892504 mutations in the ERAP2 gene, overexpression of wild-type ACE-2, TMPRSS2 and its different polymorphisms. Genes, associated with the severe course, are more common among men. According to the analysis data, it can be assumed that there are population differences. However, the diagnostic significance of the markers described must be confirmed with additional clinical studies.

scholarly journals The association between vitamin D and acute rejection in human kidney transplantation: A systematic review and meta-analysis study

2021 ◽  
pp. 101410
Author(s):  
Mohammad Mirzakhani ◽  
Sheyda Mohammadkhani ◽  
Shirin Hekmatirad ◽  
Soudabeh Aghapour ◽  
Negar Gorjizadeh ◽  
...  
Keyword(s):  
Systematic Review ◽  
Vitamin D ◽  
Kidney Transplantation ◽  
Acute Rejection ◽  
Meta Analysis ◽  
Human Kidney ◽  
Analysis Study

SECOND REPORT OF REGISTRY IN HUMAN KIDNEY TRANSPLANTATION

1964 ◽  
Vol 2 (5) ◽  
pp. 660-667 ◽  
Author(s):  
JOSEPH E. MURRAY ◽  
RAY GLEASON ◽  
ANTHONY BARTHOLOMAY
Keyword(s):  
Kidney Transplantation ◽  
Human Kidney

CURRENT EVALUATION OF HUMAN KIDNEY TRANSPLANTATION*

2006 ◽  
Vol 120 (1) ◽  
pp. 545-557 ◽  
Author(s):  
Joseph E. Murray ◽  
John P. Merrill ◽  
Gustave J. Dammin ◽  
J. Hartwell Harrison ◽  
Edward B. Hager ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Human Kidney

scholarly journals Ischemia/Reperfusion Injury in Human Kidney Transplantation

1998 ◽  
Vol 153 (2) ◽  
pp. 557-566 ◽  
Author(s):  
Dicken D.H. Koo ◽  
Kenneth I. Welsh ◽  
Justin A. Roake ◽  
Peter J. Morris ◽  
Susan V. Fuggle
Keyword(s):  
Kidney Transplantation ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Human Kidney

scholarly journals The Histological Picture of Indication Biopsies in the First 2 Weeks after Kidney Transplantation

2020 ◽  
Vol 15 (10) ◽  
pp. 1484-1493
Author(s):  
Elisabet Van Loon ◽  
Evelyne Lerut ◽  
Aleksandar Senev ◽  
Maarten Coemans ◽  
Jacques Pirenne ◽  
...  
Keyword(s):  
Risk Factors ◽  
Kidney Transplantation ◽  
Graft Failure ◽  
Proportional Hazards ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Human Kidney ◽  
Cox Proportional Hazards ◽  
Donor Age ◽  
Hazard Ratios

Background and objectivesIn preclinical studies, ischemia-reperfusion injury and older donor age are associated with graft inflammation in the early phase after transplantation. In human kidney transplantation, impaired allograft function in the first days after transplantation is often adjudicated to donor- and procedure-related characteristics, such as donor age, donor type, and ischemia times.Design, setting, participants, & measurementsIn a cohort of 984 kidney recipients, 329 indication biopsies were performed within the first 14 days after transplantation. The histologic picture of these biopsies and its relationship with alloimmune risk factors and donor- and procedure-related characteristics were studied, as well as the association with graft failure. Multivariable Cox models were applied to quantify the cause-specific hazard ratios for early rejection and early inflammatory scores, adjusted for potential confounders. For quantification of hazard ratios of early events for death-censored graft failure, landmark analyses starting from day 15 were used.ResultsEarly indication biopsy specimens displayed microvascular inflammation score ≥2 in 30% and tubulointerstitial inflammation score ≥2 in 49%. Rejection was diagnosed in 186 of 329 (57%) biopsies and associated with the presence of pretransplant donor-specific HLA antibodies and the number of HLA mismatches, but not nonimmune risk factors in multivariable Cox proportional hazards analysis. In multivariable Cox proportional hazards analysis, delayed graft function, the graft dysfunction that prompted an early indication biopsy, HLA mismatches, and pretransplant donor-specific HLA antibodies were significantly associated with a higher risk for death-censored graft failure, whereas early acute rejection was not.ConclusionsIndication biopsies performed early after kidney transplantation display inflammatory changes related to alloimmune risk factors. Nonimmune risk factors for ischemia-reperfusion injury, such as cold and warm ischemia time, older donor age, and donor type, were not identified as strong risk factors for early inflammation after human kidney transplantation.

scholarly journals 5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance

Cells ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 513 ◽  
Author(s):  
Chryssostomos Chatgilialoglu ◽  
Carla Ferreri ◽  
Nicholas E. Geacintov ◽  
Marios G. Krokidis ◽  
Yuan Liu ◽  
...  
Keyword(s):  
Genetic Instability ◽  
Model Systems ◽  
Diagnostic Significance ◽  
Purine Base ◽  
Repair Mechanisms ◽  
Cellular Dna ◽  
Radical Attack ◽  
Molecular Library

Purine 5′,8-cyclo-2′-deoxynucleosides (cPu) are tandem-type lesions observed among the DNA purine modifications and identified in mammalian cellular DNA in vivo. These lesions can be present in two diasteroisomeric forms, 5′R and 5′S, for each 2′-deoxyadenosine and 2′-deoxyguanosine moiety. They are generated exclusively by hydroxyl radical attack to 2′-deoxyribose units generating C5′ radicals, followed by cyclization with the C8 position of the purine base. This review describes the main recent achievements in the preparation of the cPu molecular library for analytical and DNA synthesis applications for the studies of the enzymatic recognition and repair mechanisms, their impact on transcription and genetic instability, quantitative determination of the levels of lesions in various types of cells and animal model systems, and relationships between the levels of lesions and human health, disease, and aging, as well as the defining of the detection limits and quantification protocols.

Sign in / Sign up

Export Citation Format

Share Document