10. HLA in Paternity Testing and Twin Classification

2015 ◽  
pp. 78-79
Keyword(s):  
Paternity Testing

Individual identification and paternity testing of bulls using microsatellite

2009 ◽  
Vol 31 (3) ◽  
pp. 285-289 ◽  
Author(s):  
Jing WANG ◽  
Chou-Sheng LIU ◽  
Li-Ping ZHANG ◽  
Zhi-Gang WANG ◽  
Fu-Qing YU ◽  
...  
Keyword(s):  
Paternity Testing ◽  
Individual Identification

Perception of the general public towards the ethical and legal issues surrounding DNA paternity testing in Lebanon

2021 ◽  
Vol 17 ◽  
pp. 100640
Author(s):  
M. Azoury ◽  
F.A. Mrad ◽  
I. Mansour ◽  
J.-N. Ibrahim ◽  
Z.A. Rjeily ◽  
...  
Keyword(s):  
General Public ◽  
Legal Issues ◽  
Paternity Testing ◽  
Ethical And Legal Issues

scholarly journals 353 ASAS-EAAP Talk: Low-coverage whole-genome sequencing in local livestock breeds

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 81-82
Author(s):  
Joaquim Casellas ◽  
Melani Martín de Hijas-Villalba ◽  
Marta Vázquez-Gómez ◽  
Samir Id Lahoucine
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Error Rate ◽  
Allele Frequencies ◽  
Paternity Testing ◽  
Sequencing Error ◽  
Whole Genome ◽  
Genomic Evaluation ◽  
Sequencing Error Rate ◽  
Low Coverage

Abstract Current European regulations for autochthonous livestock breeds put a special emphasis on pedigree completeness, which requires laboratory paternity testing by genetic markers in most cases. This entails significant economic expenditure for breed societies and precludes other investments in breeding programs, such as genomic evaluation. Within this context, we developed paternity testing through low-coverage whole-genome data in order to reuse these data for genomic evaluation at no cost. Simulations relied on diploid genomes composed by 30 chromosomes (100 cM each) with 3,000,000 SNP per chromosome. Each population evolved during 1,000 non-overlapping generations with effective size 100, mutation rate 10–4, and recombination by Kosambi’s function. Only those populations with 1,000,000 ± 10% polymorphic SNP per chromosome in generation 1,000 were retained for further analyses, and expanded to the required number of parents and offspring. Individuals were sequenced at 0.01, 0.05, 0.1, 0.5 and 1X depth, with 100, 500, 1,000 or 10,000 base-pair reads and by assuming a random sequencing error rate per SNP between 10–2 and 10–5. Assuming known allele frequencies in the population and sequencing error rate, 0.05X depth sufficed to corroborate the true father (85,0%) and to discard other candidates (96,3%). Those percentages increased up to 99,6% and 99,9% with 0,1X depth, respectively (read length = 10,000 bp; smaller read lengths slightly improved the results because they increase the number of sequenced SNP). Results were highly sensitive to biases in allele frequencies and robust to inaccuracies regarding sequencing error rate. Low-coverage whole-genome sequencing data could be subsequently integrated into genomic BLUP equations by appropriately constructing the genomic relationship matrix. This approach increased the correlation between simulated and predicted breeding values by 1.21% (h2 = 0.25; 100 parents and 900 offspring; 0.1X depth by 10,000 bp reads). Although small, this increase opens the door to genomic evaluation in local livestock breeds.

Systematic selection of microsatellite for paternity testing of yak

2021 ◽  
Author(s):  
Tong Chen ◽  
Ruilin Zeng ◽  
Wenyi Cai ◽  
Xianrong Xiong ◽  
Wei Fu ◽  
...  
Keyword(s):  
Paternity Testing ◽  
Systematic Selection ◽  
Selection Of

Simultaneous Phenotyping of Genetic Markers for Paternity Testing

1987 ◽  
Vol 99 (2) ◽  
Author(s):  
Y. Tamaki ◽  
H. Nishimukai ◽  
T. Kishida ◽  
M. Fukuda
Keyword(s):  
Genetic Markers ◽  
Paternity Testing

scholarly journals Development of EST-derived SSR Markers with Long-core Repeat in Olive and Their Use for Paternity Testing

2013 ◽  
Vol 138 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Raúl De la Rosa ◽  
Angjelina Belaj ◽  
Antonio Muñoz-Mérida ◽  
Oswaldo Trelles ◽  
Inmaculada Ortíz-Martín ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Expressed Sequence Tag ◽  
Paternity Testing ◽  
High Level ◽  
Repeated Motifs ◽  
Expressed Sequence ◽  
Simple Sequence ◽  
Olive Breeding ◽  
Genomic Project

In the present work, a set of eight new hexa-nucleotide simple sequence repeats (SSRs) is reported in olive (Olea europaea L). These SSRs loci were generated on the basis of expressed sequence tag (EST) sequences in the frame of an olive genomic project. The markers showed a high level of polymorphism when tested on a set of cultivars used as genitors in the olive breeding program of Córdoba, Spain. The long-core repeat motif of these markers allows a wider separation among alleles, thus permitting an accurate genotyping. Besides, these markers showed comparable levels of polymorphism to di-nucleotide SSRs, the only ones so far reported in olive. Selected on the basis of their discrimination capacity, four of the eight SSRs were used to test their ability for paternity testing in a total of 81 seedlings coming from 12 crosses. The paternity testing showed that seven crosses matched the alleged paternity and the remaining five were products of illicit pollinations. These results exactly matched with previous paternity testing performed with di-nucleotide SSR markers. These results demonstrate the usefulness of the developed hexa-nucleotide repeated motifs for checking the paternity of breeding progenies and suggest their use on variability studies.

scholarly journals Looking Back at Our 5-Year Experience of Paternity Testing: A Summary

2016 ◽  
Vol 40 (2) ◽  
pp. 48
Author(s):  
Moon-Young Kim ◽  
Sohee Cho ◽  
Sung Hee Lyoo ◽  
Ji Hyun Lee ◽  
Hee Jin Seo ◽  
...  
Keyword(s):  
Paternity Testing ◽  
Looking Back
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