Isoenzyme Patterns of N-Acetyl-�-Glucosaminidase from Healthy and Diseased Human Livers1

2015 ◽  
pp. 68-72
Author(s):  
G. Pott ◽  
N. van Husen ◽  
U. Gerlach
Keyword(s):  
Isoenzyme Patterns

Extra Lactate Dehydrogenase Isoenzyme Band in Serum of Patients with Severe Liver Disease

1971 ◽  
Vol 17 (9) ◽  
pp. 882-885 ◽  
Author(s):  
T Lubrano ◽  
A A Dietz ◽  
H M Rubinstein
Keyword(s):  
Liver Disease ◽  
Lactate Dehydrogenase ◽  
Serum Bilirubin ◽  
High Serum ◽  
Severe Liver ◽  
Additional Band ◽  
Dehydrogenase Isoenzyme ◽  
Severe Liver Disease ◽  
Isoenzyme Patterns ◽  
High Serum Bilirubin

Abstract In a study of lactate dehydrogenase isoenzyme patterns in the sera of patients with severe liver disease, who were primarily selected because of an abnormally high serum bilirubin, 42 of 76 patients had an additional band (LDH-T) between isoenzymes 4 and 5 on acrylamide gel. Thirty of the 42 patients died during followup, 24 within a month of recognition of the extra band.

In vitro digestion of protein sources by crude enzyme extracts of the spiny lobster Panulirus argus (Latreille, 1804) hepatopancreas with different trypsin isoenzyme patterns

Aquaculture ◽  
2010 ◽  
Vol 310 (1-2) ◽  
pp. 178-185 ◽  
Author(s):  
Erick Perera ◽  
F.J. Moyano ◽  
L. Rodriguez-Viera ◽  
A. Cervantes ◽  
G. Martínez-Rodríguez ◽  
...  
Keyword(s):  
Spiny Lobster ◽  
In Vitro Digestion ◽  
Crude Enzyme ◽  
Panulirus Argus ◽  
Protein Sources ◽  
Isoenzyme Patterns

Alkaline Phosphatase Isoenzyme Patterns in Malignant Disease

1992 ◽  
Vol 38 (12) ◽  
pp. 2546-2551 ◽  
Author(s):  
V O Van Hoof ◽  
A T Van Oosterom ◽  
L G Lepoutre ◽  
M E De Broe
Keyword(s):  
Alkaline Phosphatase ◽  
Liver Disease ◽  
Liver Metastasis ◽  
Bone Metastasis ◽  
Positive Predictive Value ◽  
Bone Disease ◽  
Predictive Value ◽  
Malignant Disease ◽  
Alp Activity ◽  
Isoenzyme Patterns

Abstract Early treatment of patients with malignant disease and liver or bone metastasis may increase their survival time. We have used the activity patterns of liver and bone isoenzymes of alkaline phosphatase (ALP), separated by agarose gel electrophoresis, to detect early metastasis. We studied ALP isoenzyme patterns in a background population of 101 patients with no evidence of any disease that might influence this pattern; a healthy reference population (n = 330); and the following three groups of patients: 143 with malignant disease, 47 with nonmalignant liver disease, and 22 with nonmalignant bone disease. Cutoff and predictive values of liver ALP, high-molecular-mass (high-M(r)) ALP, and bone ALP were established for detecting liver and bone metastasis. The positive predictive value of liver and high-M(r) ALP was higher than that of total ALP in detecting liver metastasis, but liver and high-M(r) ALP did not enable us to differentiate between malignant and nonmalignant liver disease. Total ALP activity was of slightly more value than liver and high-M(r) ALP in enabling us to rule out liver metastasis. From bone ALP activity we could not distinguish between nonmalignant bone disease and bone metastasis. The negative predictive value of bone ALP in the diagnosis of bone metastasis was low, but its positive predictive value was high and superior to that of total ALP.

scholarly journals How the sialylation level of serum N-acetyl-β-D-glucosaminidase a form in type 1 diabetes mellitus influences its activity?

2014 ◽  
Vol 79 (12) ◽  
pp. 1491-1503
Author(s):  
Vesna Jovanovic ◽  
Jelena Acimovic ◽  
Vesna Dimitrijevic-Sreckovic ◽  
Ljuba Mandic
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Structural Changes ◽  
Negative Charge ◽  
Total Serum ◽  
Secondary Complications ◽  
Isoenzyme Patterns ◽  
Healthy Persons

It has been verified that serum N-acetyl-?-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes of the sialic acid (SA) content in the carbohydrate parts of NAG A form and its influence on total NAG activity changes in type 1 diabetes mellitus patients without and with secondary complications. NAG A forms were isolated, purified and characterized from the serum of 81 IDDM patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons. The content of ?-2,6-bound SA and isoenzyme patterns of purified A form, total NAG and A form activities were determined. In all diabetic groups, A form sialylation levels were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P<0.001) in all diabetic groups and negatively correlated with the ?-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. Observed changes could be the consequence of structural changes in the A form due to significant increase in its acidity, i.e. negative charge which originate from groups other than SA.

scholarly journals Analysis of lactate dehydrogenase activities and isoenzyme patterns in colorectal cancer tissues

2011 ◽  
Vol 3 (1) ◽  
pp. 41
Author(s):  
Chun-Hua Zhao ◽  
Chun-Ying Jiang ◽  
Yu-Yi Zhang ◽  
Xian-Xi Liu ◽  
Dao-Chun Luo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Lactate Dehydrogenase ◽  
Cancer Tissues ◽  
Isoenzyme Patterns

ACIDIC PEROXIDASES OF APPLE ROOTSTOCKS

1987 ◽  
Vol 67 (1) ◽  
pp. 293-297 ◽  
Author(s):  
DAVID L. WELLER
Keyword(s):  
Molecular Weight ◽  
Isoelectric Focusing ◽  
Peroxidase Activity ◽  
Sucrose Gradient ◽  
Bark Extract ◽  
Root Extract ◽  
Apple Rootstock ◽  
Apple Rootstocks ◽  
Gel Isoelectric Focusing ◽  
Isoenzyme Patterns

The peroxidase activity in extracts of shoot bark from Michigan State Apple Clone (MAC) 24 and in extracts of roots of MAC 24, East Mailing Long Ashton (EMLA) 27, and Oregon Apple Rootstock (OAR) 1 were characterized as being primarily acidic by broad range sucrose gradient isoelectric focusing. Gel isoelectric focusing (GIEF) using a narrower conventional or immobilized pH gradient showed that this activity could be separated into about a dozen isoenzymes. The GIEF isoenzyme patterns of MAC 24 shoot bark extract peroxidase and root extract peroxidases of MAC 24, EMLA 27, and OAR 1 differed. Similar values were obtained for the molecular weight of the peroxidase activity of these extracts. The results indicate that the acidic isoenzymes of apple peroxidase are of similar size.Key words: Apple, rootstocks (apple), peroxidase, molecular weight, isoenzymes

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