Kinetic Aspects of Purine Metabolism in Cultured Fibroblasts

2015 ◽  
pp. 96-99 ◽  
Author(s):  
E. Zoref ◽  
A. de Vries ◽  
O. Sperling
Keyword(s):  
Purine Metabolism ◽  
Cultured Fibroblasts

Intralysosomal Accumulation of Colloidal Gold-Low Density Lipoprotein Conjugates in Chloroquine-Treated Fibroblasts

1985 ◽  
Vol 43 ◽  
pp. 546-547 ◽  
Author(s):  
Dean A. Handley ◽  
Cynthia M. Arbeeny ◽  
Larry D. Witte
Keyword(s):  
Colloidal Gold ◽  
Cultured Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coated Vesicle ◽  
Low Density ◽  
Cholesterol Esters ◽  
Cultured Fibroblasts ◽  
Surface Receptors ◽  
Ldl Particles

Low density lipoproteins (LDL) are the major cholesterol carrying particles in the blood. Using cultured cells, it has been shown that LDL particles interact with specific surface receptors and are internalized via a coated pit-coated vesicle pathway for lysosomal catabolism. This (Pathway has been visualized using LDL labeled to ferritin or colloidal gold. It is now recognized that certain lysomotropic agents, such as chloroquine, inhibit lysosomal enzymes that degrade protein and cholesterol esters. By interrupting cholesterol ester hydrolysis, chloroquine treatment results in lysosomal accumulation of cholesterol esters from internalized LDL. Using LDL conjugated to colloidal gold, we have examined the ultrastructural effects of chloroquine on lipoprotein uptake by normal cultured fibroblasts.

scholarly journals Synthesis and release of procollagenase by cultured fibroblasts.

1976 ◽  
Vol 251 (10) ◽  
pp. 3162-3168 ◽  
Author(s):  
H Birkedal-Hansen ◽  
C M Cobb ◽  
R E Taylor ◽  
H M Fullmer
Keyword(s):  
Cultured Fibroblasts

scholarly journals Vitamin B12 and Purine Metabolism in Lactobacillus leichmannii

1963 ◽  
Vol 238 (4) ◽  
pp. 1464-1466
Author(s):  
Gary R. Craven ◽  
Mancourt Downing
Keyword(s):  
Vitamin B12 ◽  
Purine Metabolism ◽  
Lactobacillus Leichmannii
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