Metabolism of Cyclic Nucleotides in Healthy and Diseased Human Muscle

2015 ◽  
pp. 63-67
Author(s):  
N. Canal ◽  
C. Cerri ◽  
B. Musch ◽  
L. Frattola
Keyword(s):  
Cyclic Nucleotides ◽  
Human Muscle

Isolation and characterization of the human muscle glycogen synthase gene

Diabetes ◽  
1995 ◽  
Vol 44 (9) ◽  
pp. 1099-1105 ◽  
Author(s):  
M. Orho ◽  
P. Nikula-Ijas ◽  
C. Schalin-Jantti ◽  
M. A. Permutt ◽  
L. C. Groop
Keyword(s):  
Muscle Glycogen ◽  
Glycogen Synthase ◽  
Human Muscle ◽  
Isolation And Characterization

scholarly journals The Regulation of Lipogenesis by Cyclic Nucleotides in Intact Hepatocytes Prepared by a Simplified Technique

1974 ◽  
Vol 249 (4) ◽  
pp. 1286-1294
Author(s):  
David M. Capuzzi ◽  
Vicki Rothman ◽  
Simeon Margolis
Keyword(s):  
Cyclic Nucleotides

scholarly journals Inflammatory cytokines-stimulated human muscle stem cells ameliorate ulcerative colitis via the IDO-TSG6 axis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shengchao Zhang ◽  
Jiankai Fang ◽  
Zhanhong Liu ◽  
Pengbo Hou ◽  
Lijuan Cao ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Inflammatory Cytokines ◽  
Human Muscle ◽  
Enzyme Linked Immunosorbent Assay ◽  
Muscle Stem Cells ◽  
Tnf Α ◽  
Ifn Γ ◽  
Anti Inflammatory

Abstract Background Muscle stem cells (MuSCs) are absolutely required for the formation, repair, and regeneration of skeletal muscle tissue. Increasing evidence demonstrated that tissue stem cells, especially mesenchymal stem cells (MSCs), can exert therapeutic effects on various degenerative and inflammatory disorders based on their immunoregulatory properties. Human mesenchymal stem cells (hMSCs) treated with interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were reported to possess anti-inflammatory functions by producing TNF-stimulated gene 6 (TSG-6). However, whether human muscle stem cells (hMuSCs) also possess TSG-6 mediated anti-inflammatory functions has not been explored. Methods The ulcerative colitis mouse model was established by subjecting mice to dextran sulfate sodium (DSS) in drinking water for 7 days. hMuSCs were pretreated with IFN-γ and TNF-α for 48 h and were then transplanted intravenously at day 2 of DSS administration. Body weights were monitored daily. Indoleamine 2,3-dioxygenase (IDO) and TSG-6 in hMuSCs were knocked down with short hairpin RNA (shRNA) and small interfering RNA (siRNA), respectively. Colon tissues were collected for length measurement and histopathological examination. The serum level of IL-6 in mice was measured by enzyme-linked immunosorbent assay (ELISA). Real-time PCR and Western blot analysis were performed to evaluate gene expression. Results hMuSCs treated with inflammatory factors significantly ameliorated inflammatory bowel disease (IBD) symptoms. IDO and TSG-6 were greatly upregulated and required for the beneficial effects of hMuSCs on IBD. Mechanistically, the tryptophan metabolites, kynurenine (KYN) or kynurenic acid (KYNA) produced by IDO, augmented the expression of TSG-6 through activating their common receptor aryl hydrocarbon receptor (AHR). Conclusion Inflammatory cytokines-treated hMuSCs can alleviate DSS-induced colitis through IDO-mediated TSG-6 production.

scholarly journals Alternative splicing of the transcript encoding the human muscle isoenzyme of phosphofructokinase.

1990 ◽  
Vol 265 (16) ◽  
pp. 9006-9010 ◽  
Author(s):  
P M Sharma ◽  
G R Reddy ◽  
B M Babior ◽  
A McLachlan
Keyword(s):  
Alternative Splicing ◽  
Human Muscle
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