On the Age-Associated Presence of Immunoglobulin and Complement in the Renal Glomeruli of Mice

2015 ◽  
pp. 79-93 ◽  
Author(s):  
Fujio Shimizu ◽  
Fumi Abe ◽  
Ito Kinji ◽  
Sadao Kawamura
Keyword(s):  
Renal Glomeruli

A Re-Appraisal of the Role of Blood Coagulation and Platelets in the Generalized Shwartzman Phenomenon

1966 ◽  
Vol 15 (03/04) ◽  
pp. 519-538 ◽  
Author(s):  
J Levin ◽  
E Beck
Keyword(s):  
Blood Coagulation ◽  
The State ◽  
Coagulation System ◽  
Renal Lesions ◽  
Intravascular Coagulation ◽  
Renal Glomeruli ◽  
Coagulation Mechanism ◽  
Shwartzman Phenomenon ◽  
Do So

SummaryThe role of intravascular coagulation in the production of the generalized Shwartzman phenomenon has been evaluated. The administration of endotoxin to animals prepared with Thorotrast results in activation of the coagulation mechanism with the resultant deposition of fibrinoid material in the renal glomeruli. Anticoagulation prevents alterations in the state of the coagulation system and inhibits development of the renal lesions. Platelets are not primarily involved. Platelet antiserum produces similar lesions in animals prepared with Thorotrast, but appears to do so in a manner which does not significantly involve intravascular coagulation.The production of adrenal cortical hemorrhage, comparable to that seen in the Waterhouse-Friderichsen syndrome, following the administration of endotoxin to animals that had previously received ACTH does not require intravascular coagulation and may not be a manifestation of the generalized Shwartzman phenomenon.

Measurement of expansion factor and distortion for expansion microscopy using isolated renal glomeruli as landmarks

2021 ◽  
Author(s):  
Chen Zhu ◽  
Aidong Wang ◽  
Lili Chen ◽  
Liangsheng Guo ◽  
Jiajia Ye ◽  
...  
Keyword(s):  
Expansion Factor ◽  
Renal Glomeruli

scholarly journals Renal Glomeruli and Tubular Injury Following Indomethacin, Ibuprofen, and Gentamicin Exposure in a Neonatal Rat Model

2007 ◽  
Vol 62 (3) ◽  
pp. 307-312 ◽  
Author(s):  
Alison L Kent ◽  
Lesley E Maxwell ◽  
Mark E Koina ◽  
Michael C Falk ◽  
David Willenborg ◽  
...  
Keyword(s):  
Rat Model ◽  
Neonatal Rat ◽  
Tubular Injury ◽  
Renal Glomeruli ◽  
Neonatal Rat Model

Fourier-based quantification of renal glomeruli size using Hough transform and shape descriptors

2017 ◽  
Vol 151 ◽  
pp. 179-192 ◽  
Author(s):  
Sohrab Najafian ◽  
Borhan Beigzadeh ◽  
Mohammad Riahi ◽  
Fatemeh Khadir Chamazkoti ◽  
Mahdi Pouramir
Keyword(s):  
Hough Transform ◽  
Shape Descriptors ◽  
Renal Glomeruli

Isolation of Renal Glomeruli Specific Cell Material Using an Experimental NIR-Laser Microdissection Setup

2002 ◽  
Vol 17 (1) ◽  
pp. 21-24
Author(s):  
Cathrin Dressler ◽  
Markus Wehland ◽  
Jürgen Beuthan ◽  
Reinhold Kreutz ◽  
Martin Paul ◽  
...  
Keyword(s):  
Laser Microdissection ◽  
Specific Cell ◽  
Cell Material ◽  
Renal Glomeruli ◽  
Nir Laser

scholarly journals Diagnostic Value of Type IV Collagen Expression in Renal Glomeruli at Alport’s Syndrome

2021 ◽  
Vol 65 (6) ◽  
pp. 42-49
Author(s):  
M. E. Aksenova ◽  
P. E. Povilaitite ◽  
N. E. Konkova ◽  
V. V. Dlin
Keyword(s):  
Electron Microscopy ◽  
Type Iv Collagen ◽  
Immunohistochemical Study ◽  
Immunohistochemical Method ◽  
Alport's Syndrome ◽  
Alport’S Syndrome ◽  
Type Iv ◽  
Renal Glomeruli ◽  
Syndrome Diagnosis

The Alport’s syndrome is the hereditary multisystem disease characterized by the development of the progressive nephropathy. The early diagnosis and subsequent prescription of nephroprotective therapy improves significantly the nephrological prognosis. Purpose of the Study. Determine the value of the immunohistochemical method for the Alport’s syndrome diagnosis. Material and methods. The clinical, laboratory and morphological data of 35 patients with suspected Alport’s syndrome (13 years of age [11; 16]; 18 boys and 17 girls) examined in the Nephrology Department in 2013–2019 were summarized. The study of the renal tissue included the light, immunofluorescence, electron microscopy of the kidney biopsy sample, determination of the expression of α1, α3 and α5 chains of type IV collagen in the renal glomeruli using the immunohistochemical method; the genetic testing was carried out for 26 patients. The children were divided into groups depending on the glomerular expression of α5 chain of type IV collagen: normal (group 1, n=18), decreased (group 2, n=4), negative (group 3, n=13). Results are as the following: The disorder of the expression of α5 chain was detected in ¾ (q = 0.78) patients with genetically confirmed Alport’s syndrome and in almost all children with the X-linked variant of the disease (q = 0.94). Results. Based on the genetic testing, the Alport’s syndrome was confirmed in ¼ of the children of the 1st group (the children with the heterozygous variants of COL4A3, COL4A5 genes) and in all children of the 2nd and 3rd groups (COL4A5 variants). The sensitivity/ specificity of the immunohistochemical study for the Alport’s syndrome diagnosis was 78% /100%, that of the electron microscopy – 93% /87%. The predictive value of the positive/negative result of the immunohistochemical study was 100% /66%, that of the electron microscopy – 95% / 88% compared with 100% / 88% with the combine use of two methods. Conclusion. The determination of the expression of α5 chain of type IV collagen in the renal glomeruli has the independent diagnostic value, but it is inferior to the electron microscopy in the heterozygous variants of the Alport’s syndrome. The high specificity of the immunohistochemical method makes it possible to confirm the Alport’s syndrome in the case of the change in the expression of α5 chain of type IV collagen in the renal glomeruli.

scholarly journals MicroRNA-29a Attenuates Diabetic Glomerular Injury through Modulating Cannabinoid Receptor 1 Signaling

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 264 ◽  
Author(s):  
Chun-Wu Tung ◽  
Cheng Ho ◽  
Yung-Chien Hsu ◽  
Shun-Chen Huang ◽  
Ya-Hsueh Shih ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Cannabinoid Receptors ◽  
Mesangial Cells ◽  
Kidney Diseases ◽  
Negative Regulator ◽  
Glomerular Injury ◽  
Renal Hypertrophy ◽  
Cannabinoid Type 1 Receptor ◽  
Ppar Γ ◽  
Renal Glomeruli

Diabetic nephropathy often leads to end-stage renal disease and life-threatening morbidities. Simple control of risk factors is insufficient to prevent the progression of diabetic nephropathy, hence the need for discovering new treatments is of paramount importance. Recently, the dysregulation of microRNAs or the cannabinoid signaling pathway has been implicated in the pathogenesis of various renal tubulointerstitial fibrotic damages and thus novel therapeutic targets for chronic kidney diseases have emerged; however, the role of microRNAs or cannabinoid receptors on diabetes-induced glomerular injuries remains to be elucidated. In high-glucose-stressed renal mesangial cells, transfection of a miR-29a precursor sufficiently suppressed the mRNA and protein expressions of cannabinoid type 1 receptor (CB1R). Our data also revealed upregulated CB1R, interleukin-1β, interleukin-6, tumor necrosis factor-α, c-Jun, and type 4 collagen in the glomeruli of streptozotocin (STZ)-induced diabetic mice, whereas the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) was decreased. Importantly, using gain-of-function transgenic mice, we demonstrated that miR-29a acts as a negative regulator of CB1R, blocks the expressions of these proinflammatory and profibrogenic mediators, and attenuates renal hypertrophy. We also showed that overexpression of miR-29a restored PPAR-γ signaling in the renal glomeruli of diabetic animals. Collectively, our findings indicate that the interaction between miR-29a, CB1R, and PPAR-γ may play an important role in protecting diabetic renal glomeruli from fibrotic injuries.

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