Immunofluorescent Test for the Detection of Anti-HBC

2015 ◽  
pp. 65-70
Author(s):  
K. Madalinski ◽  
A. Budkowska ◽  
T. Michalak ◽  
C. Trepo
Keyword(s):  
Immunofluorescent Test

scholarly journals Direct immunofluorescent test for the detection of gonorrhoea.

1976 ◽  
Vol 52 (1) ◽  
pp. 33-35
Author(s):  
J Klanica ◽  
M Stejskalova
Keyword(s):  
Immunofluorescent Test

Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults

1979 ◽  
Vol 9 (6) ◽  
pp. 688-692
Author(s):  
J A Daisy ◽  
F S Lief ◽  
H M Friedman
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Virus Isolation ◽  
Fluorescent Antibody ◽  
Positive Culture ◽  
H3n2 Virus ◽  
Direct Immunofluorescence ◽  
The One ◽  
Immunofluorescent Test ◽  
Culture Negative

The efficacy for direct immunofluorescence of a commercial conjugate for influenza A virus prepared against whole A/Udorn (H3NS) virus was studied. The conjugate was specific for influenza A virus, but its sensitivity varied depending upon the strain of influenza A tested. Nasopharyngeal aspirates collected from 25 patients during an outbreak of influenza were examined for viral antigen with the conjugates and inoculated onto monkey kidney (MK) cells for virus isolation. Fifteen patients had isolates for influenza A/USSR/90/77 (H1N1); nasopharyngeal secretions were fluorescent antibody positive in 12. Fluorescent antibody was copositive with culture in 11/15 patients (73.3%) and conegative in 9/10 (90%). The one fluorescent antibody-positive, culture-negative patient had negative serology for influenza A and the fluorescent antibody result was considered to be a false positive. At a 1:10 dilution, the conjugate stained nasopharyngeal and MK cells infected with A/USSR (H1N1) 2 to 3+, whereas cells infected with H3N2 virus stained 4+. A conjugate made specifically against the ribonucleoprotein antigen, which is universal to all influenza A strains, may improve the sensitivity of the direct immunofluorescent test.

scholarly journals Feline bartonellosis· A review

2017 ◽  
Vol 63 (1) ◽  
pp. 63 ◽  
Author(s):  
L. V. ATHANASIOU (Λ.Β. ΑΘΑΝΑΣΙΟΥ) ◽  
M. K. CHATZIS (Μ.Κ. ΧΑΤΖΗΣ) ◽  
I. V. KONTOU (Ι. B. ΚΟΝΤΟΥ) ◽  
V. I. KONTOS (Β. I. ΚΟΝΤΟΣ) ◽  
V. SPYROU (B. ΣΠΥΡΟΥ)
Keyword(s):  
Direct Detection ◽  
Bartonella Henselae ◽  
Effective Means ◽  
Human Infection ◽  
Bacterial Pathogenicity ◽  
Mild Anemia ◽  
Flea Control ◽  
Hepatic Peliosis ◽  
Accidental Host ◽  
Immunofluorescent Test

Bartonella infection is caused by Gram negative bacteria commonly isolated from domestic cats. Cats are the major reservoir of Bartonella henselae, B. clarridgeiae and B. koehlerae which are transmitted to humans, while they are accidental host of B, quintana. Β, bovis και Β, vìmonìi subsp. berkhoffìi. The pathogen is transmitted among cats mainlyby fleas while other vectors are also suspicious for transmission since the bacteria have been isolated from ticks and flies.The bacterial pathogenicity may be emphasized by the strain of the bacterium and the immune status of the infected host. Most of the infected eats remain asymptomatic. In the natural occurring cases of feline bartonellosis uveitis, chronic gingivostomatitis and endocarditis have been reported. Mild anemia and leucocitosi s in the early phase of the infection has been also reported. Diagnosis is based on the detection of the specific anti-bartoneila antibodies by the inderict immunofluorescent test, ELISA and Western blot assays. Molecular biology techniques mainly PCR, cytology, histopathology and blood culture have also been employed for the direct detection of the pathogen. Prolonged antimicrobial therapy results to the reduction of bacterial burden without total elimination of the pathogen.Bartonella henselae is the causative agent of cat scratch disease, a human infection usually characterized by persistent regional lymphadenopathy and less frequently fever while angiomatosis or hepatic peliosis have been reported mainly in immunocompromised patients. It is transmitted to humans by cat scratches or bites. The most effective means of protection is regular flea control Additionally, commonsense precautions and hygiene such as washing hands after handling pets and clean any cats and bites or scratches promptly are recommended especially in population at great risk.

scholarly journals Peroxidase antibody test for mucocutaneous leishmaniasis serology performance indexes and comparison with a fluorescent antibody test: Comparação com o desempenho da reação de imunofluorescência

1988 ◽  
Vol 30 (6) ◽  
pp. 411-414 ◽  
Author(s):  
Beatriz J. Celeste ◽  
M. Carolina S. Guimarães ◽  
Edelma Maria Corrales L.
Keyword(s):  
Visceral Leishmaniasis ◽  
Positive Predictive Value ◽  
Chagas Disease ◽  
Predictive Value ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Protein Ratio ◽  
Technical Problems ◽  
Performance Indexes ◽  
Immunofluorescent Test

Performance indexes of the peroxidase antibody test were compared to that of the fluorescent antibody test. The peroxidase antibody test had a statistically higher sensitivity and negative predictive value and a higher efficiency than the fluorescent antibody test but its specificity and positive predictive value were within the 95% confidence limits for the values found for the fluorescent antibody test. Such differences did not change when Chagas' disease and visceral leishmaniasis sera were included in index calculations. Statistical analysis showed that the two tests have a substantial degree of agreement but the immunofluorescent test had a specificity index and a positive predictive value equal to 100.0% when Chagas' disease and visceral leishmaniasis sera were not included in the calculations of the performance index; in this instance, a positive test result equals a disclosure of the disease attribute due to the inexistence of false positive results. The enzyme/ protein ratio of the peroxidase conjugate, resulting in heavy or light-labeled conjugates may pose technical problems to its use in serology tests.

scholarly journals Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

1987 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Amauri Braga Simonetti ◽  
Jorge Teixeira
Keyword(s):  
Cerebrospinal Fluid ◽  
Indirect Immunofluorescence ◽  
False Positive ◽  
Clinical Evidence ◽  
Routine Analysis ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Immunofluorescent Test ◽  
Positive Results

Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocitosis in 88.7% of cases, eosinophilorrachia in 60.3%o, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 19.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

scholarly journals CORRELATION OF ANA IMMUNOFLUOROSCENT TITRE AND PATTERN DISTRIBUTION WITH CLINICAL FEATURES: JOURNEY BEYOND SLE

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Dr. Pooja Prapanna ◽  
Dr. Neelam Bharihoke
Keyword(s):  
Clinical Features ◽  
Unknown Origin ◽  
Autoimmune Disorder ◽  
Pyrexia Of Unknown Origin ◽  
Normal Individuals ◽  
Pattern Distribution ◽  
One Year ◽  
Immunofluorescent Test ◽  
Indirect Immunofluorescent ◽  
Positive Results

This is a Retrospective study conducted at Pathology department Bombay Hospital Indore. 300 patients were tested for presence of ANA antibody using indirect immunofluorescent test (IMMUNOSHOP AESKU SLIDES) over the period of one year. ANA testing by IIF is a highly valuable and time tested technique for diagnosis of autoimmune disorder.  Results should be interpretated in the light of clinical and biochemical findings as normal individuals have positive results on traditional ANA testing. The most definitive result from ANA testing is a negative test. This result, especially when coupled with negative tests on an ANA profile, suggests strongly that ANA associated diseases are unlikely to be present. This imparts a high NPV to ANA IIF tests. Apart from the usually described clinical features this study highlights few of the uncommon isolated clinical features like cytopenias, myopathies and Pyrexia of unknown origin and utility of ANA IIF in establishing diagnosis. We at our centre perform ANA profile of patients to further classify the disease which is beyond the scope of this article. Keywords: ANA, Immunofluoroscent, Titre & SLE

scholarly journals Neonatal alloimmune thrombocytopenia: detection and characterization of the responsible antibodies by the platelet immunofluorescence test

Blood ◽  
1981 ◽  
Vol 57 (4) ◽  
pp. 649-656
Author(s):  
AE von dem Borne ◽  
EF van Leeuwen ◽  
LE von Riesz ◽  
CJ van Boxtel ◽  
CP Engelfriet
Keyword(s):  
Cell Types ◽  
Small Scale ◽  
Cytotoxicity Test ◽  
Platelet Antibodies ◽  
Scale Population ◽  
Mother And Child ◽  
Lymphocyte Cytotoxicity ◽  
Specific Igg ◽  
Immunofluorescent Test ◽  
Alloimmune Thrombocytopenia

Platelet immunofluorescence, together with other serologic tests on platelets, lymphocytes, and granulocytes, was used to investigate the sera of 38 mothers with newborns who suffered from thrombocytopenia. In sera of 33 mothers, platelet-specific IgG alloantibodies were demonstrable. Three sera also contained HLA antibodies, of which two were only detectable in the lymphocyte cytotoxicity test. Two other sera contained granulocyte-specific alloantibodies. In sera of 2 mothers, antibodies were found that reacted with all cell types in all tests. However, after further analysis, it became clear that platelet- specific alloantibodies were probably also present in these 2 sera. In 29 cases, the specificity of the platelet alloantibodies was anti-Zwa-- PlA1. One serum contained antibodies directed against a new antigen, Baka. This new antigen was defined after the investigation of the family and a small-scale population study. Two other sera had platelet antibodies with still undefined specificities. In all positive sera, IgG platelet alloantibodies were detected, and sometimes IgM antibodies were also present. The IgG antibodies were mostly of the IgG1 subclasses, but sometimes IgG3 and/or IgG4 was also found. In a few sera, only IgG3 antibodies were detected. In our series, we found no increased frequency of blood group ABO compatibility between mother and child, although it has been described by others and is well known to occur in rhesus alloimmunization. Of all the tests used, the platelet immunofluorescent test, especially the test on paraformaldehyde-fixed platelets in suspension, gave the best results in the detection of platelet antibodies in neonatal alloimmune thrombocytopenia.

scholarly journals Neonatal alloimmune thrombocytopenia: detection and characterization of the responsible antibodies by the platelet immunofluorescence test

Blood ◽  
1981 ◽  
Vol 57 (4) ◽  
pp. 649-656 ◽  
Author(s):  
AE von dem Borne ◽  
EF van Leeuwen ◽  
LE von Riesz ◽  
CJ van Boxtel ◽  
CP Engelfriet
Keyword(s):  
Cell Types ◽  
Small Scale ◽  
Cytotoxicity Test ◽  
Platelet Antibodies ◽  
Scale Population ◽  
Mother And Child ◽  
Lymphocyte Cytotoxicity ◽  
Specific Igg ◽  
Immunofluorescent Test ◽  
Alloimmune Thrombocytopenia

Abstract Platelet immunofluorescence, together with other serologic tests on platelets, lymphocytes, and granulocytes, was used to investigate the sera of 38 mothers with newborns who suffered from thrombocytopenia. In sera of 33 mothers, platelet-specific IgG alloantibodies were demonstrable. Three sera also contained HLA antibodies, of which two were only detectable in the lymphocyte cytotoxicity test. Two other sera contained granulocyte-specific alloantibodies. In sera of 2 mothers, antibodies were found that reacted with all cell types in all tests. However, after further analysis, it became clear that platelet- specific alloantibodies were probably also present in these 2 sera. In 29 cases, the specificity of the platelet alloantibodies was anti-Zwa-- PlA1. One serum contained antibodies directed against a new antigen, Baka. This new antigen was defined after the investigation of the family and a small-scale population study. Two other sera had platelet antibodies with still undefined specificities. In all positive sera, IgG platelet alloantibodies were detected, and sometimes IgM antibodies were also present. The IgG antibodies were mostly of the IgG1 subclasses, but sometimes IgG3 and/or IgG4 was also found. In a few sera, only IgG3 antibodies were detected. In our series, we found no increased frequency of blood group ABO compatibility between mother and child, although it has been described by others and is well known to occur in rhesus alloimmunization. Of all the tests used, the platelet immunofluorescent test, especially the test on paraformaldehyde-fixed platelets in suspension, gave the best results in the detection of platelet antibodies in neonatal alloimmune thrombocytopenia.

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