Signals Regulating in vitro Activation of Lymphocytes

2015 ◽  
pp. 65-194
Author(s):  
Joost J. Oppenheim ◽  
David L. Rosenstreich
Keyword(s):  
In Vitro Activation

scholarly journals Purification of the unactivated glucocorticoid receptor and its subsequent in vitro activation.

1984 ◽  
Vol 259 (5) ◽  
pp. 3173-3180 ◽  
Author(s):  
P Grandics ◽  
A Miller ◽  
T J Schmidt ◽  
D Mittman ◽  
G Litwack
Keyword(s):  
Glucocorticoid Receptor ◽  
In Vitro Activation

Vitamin D modulates the expression of HLA-DR and CD38 after in vitro activation of T-cells

2017 ◽  
Vol 29 (3) ◽  
Author(s):  
Simon Villegas-Ospina ◽  
Wbeimar Aguilar-Jimenez ◽  
Sandra M. Gonzalez ◽  
María T. Rugeles
Keyword(s):  
Vitamin D ◽  
Flow Cytometry ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Activation Markers ◽  
Hla Dr ◽  
In Vitro Activation ◽  
Cells Cultured

AbstractObjective:Vitamin D (VitD) is an anti-inflammatory hormone; however, some evidence shows that VitD may induce the expression of activation markers, such as CD38 and HLA-DR. We explored its effect on the expression of these markers on CD4Materials and methods:CD38 and HLA-DR expression was measured by flow cytometry in PHA/IL-2-activated mononuclear cells cultured under VitD precursors: three cholecalciferol (10Results:Cholecalciferol at 10Conclusion:Although no significant correlations were observed in vivo in healthy subjects, VitD treatment in vitro modulated immune activation by increasing the expression of CD38 and decreasing the proliferation of HLA-DR

Diploid and haploid mouse parthenogenetic development following in vitro activation and embryo transfer

Development ◽  
1974 ◽  
Vol 31 (3) ◽  
pp. 635-642
Author(s):  
M. H. Kaufman ◽  
R. L. Gardner
Keyword(s):  
Embryo Transfer ◽  
Mouse Embryos ◽  
Parthenogenetic Development ◽  
In Vitro Activation ◽  
Haploid Embryos ◽  
Parthenogenetic Mouse Embryos

Parthenogenetic mouse embryos were selected following in vitro activation, and transferred to the oviducts of pseudopregnant recipients. Decidua was evoked by 50–56% of diploid parthenogenones compared to 35·1% of haploid embryos with a single pronucleus, 37·5% of immediate cleavage eggs and 77% of fertilized eggs (controls). On day 4, 58·7% of diploid parthenogenones were morphologically normal morulae or blastocysts; over 90% of these ‘normal’ embryos evoked decidua when retransferred to recipients compared to 8·9% of abnormal embryos flushed from the ‘transfer’ sides, suggesting that only ‘normal’ embryos could evoke decidua. Potentially diploid parthenogenones remained diploid on chromosomal examination on day 4.

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