EB Virus : Malignant Lymphoma in Cottontop Marmosets Following Inoculation and Recovery of the Virus from Cells of an Experimental Tumor Maintained in Organ Culture

2015 ◽  
pp. 375-383
Author(s):  
T. C. Shope ◽  
G. Miller
Keyword(s):  
Organ Culture ◽  
Malignant Lymphoma ◽  
Experimental Tumor ◽  
Eb Virus

B-cell nature malignant lymphoma probably caused by EB-virus infection

1978 ◽  
Vol 378 (4) ◽  
pp. 321-328
Author(s):  
Iwao Emura ◽  
Yoshihisa Ohnishi ◽  
Syoichi Oboshi
Keyword(s):  
Virus Infection ◽  
B Cell ◽  
Malignant Lymphoma ◽  
Eb Virus

scholarly journals Lymphoma in cotton-top marmosets after inoculation with Epstein-Barr virus: tumor incidence, histologic spectrum antibody responses, demonstration of viral DNA, and characterization of viruses.

1977 ◽  
Vol 145 (4) ◽  
pp. 948-967 ◽  
Author(s):  
G Miller ◽  
T Shope ◽  
D Coope ◽  
L Waters ◽  
J Pagano ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Malignant Lymphoma ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Transformed Cells ◽  
Barr Virus ◽  
Eb Virus ◽  
Inapparent Infection ◽  
Human Sera ◽  
Epstein Barr

6 of 20 cotton-top tamarins (Saguinus oedipus) inoculated with Epstein-Barr virus (EBV) developed diffuse malignant lymphoma resembling reticulum cell or immunoblastic sarcoma of man. Hyperplastic lymphoreticular lesions were induced in three additional animals; in two instances the hyperplastic lesions regressed. Inapparent infection with development of antibody occured in eight animals. In two animals there was no evidence of EBV infection. One animal died in the first week after inoculation of parasitic infection. 10 animals uninoculated or mock-inoculated developed neither lymphoproliferative disease nor antibody. The malignant lymphoma appeared to arise from a cell with an uncleaved vesicular nucleus found in the center of the germinal follicle. The prominent cytologic features of this cell were extensive formation or rough endoplasmic reticulum and elaboration of the cytoplasmic membrane with microvilli. Cell lines derived from these tumors did not have receptors for complement. IgFc, or sheep erythrocytes, and the cell lines adhered to glass and plastic. EB nuclear antigen was found in imprints of two lymph nodes, one with lymphoma and one with hyperplasia. EB virus DNA was detected directly in the tumors of three animals and in cell lines from two lymphomas. Typical herpes virus particles were found in supernatant fluids from cell lines obtained from lymph nodes with tumors and hyperplasia, as well as in lines derived from blood leukocytes of marmosets with inapparent infection. These virus preparations had the biologic property characteristic of EBV, namely, stimulation of cellular DNA synthesis and immortalization of human lymphocytes. The virus derived from two cell lines was neutralized by reference human sera with EBV antibody and not by antibody-negative human sera. The virus derived from the experimental lesions is thus indistinghishable from human EBV. The marmoset has enhanced susceptibility to oncogenesis by EB virus. Among identified factors which may play a role in the heightened tumorigenicity of EB virus in this species are the increased production of virus by transformed cells and the absence of membrane receptors for complement or IgFc on transformed cells.

Adenocarcinoma of the breast metastatic to malignant lymphoma

JAMA ◽  
1966 ◽  
Vol 195 (11) ◽  
pp. 963-965 ◽  
Author(s):  
G. L. Woolam
Keyword(s):  
Malignant Lymphoma

Freeze-etch observations on the tight junctions of sertoli cells grown in organ culture with FSH

1978 ◽  
Vol 36 (2) ◽  
pp. 340-341
Author(s):  
Rita Meyer ◽  
Zoltan Posalaky ◽  
Dennis Mcginley
Keyword(s):  
Organ Culture ◽  
Tight Junctions ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Barrier Function ◽  
Cell Junction ◽  
Intramembranous Particles ◽  
Junctional Complexes ◽  
Oriented Parallel

The Sertoli cell tight junctional complexes have been shown to be the most important structural counterpart of the physiological blood-testis barrier. In freeze etch replicas they consist of extensive rows of intramembranous particles which are not only oriented parallel to one another, but to the myoid layer as well. Thus the occluding complex has both an internal and an overall orientation. However, this overall orientation to the myoid layer does not seem to be necessary to its barrier function. The 20 day old rat has extensive parallel tight junctions which are not oriented with respect to the myoid layer, and yet they are inpenetrable by lanthanum. The mechanism(s) for the control of Sertoli cell junction development and orientation has not been established, although such factors as the presence or absence of germ cells, and/or hormones, especially FSH have been implicated.

Application of Transmission Electron Microscope and Scanning Electron Microscope in experimental tumor studies

1990 ◽  
Vol 48 (3) ◽  
pp. 306-307
Author(s):  
Gao Fengming
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Malignant Transformation ◽  
Transmission Electron Microscope ◽  
Experimental Tumor ◽  
Transmission Electron ◽  
Embryo Cells ◽  
Tumor Studies ◽  
Scanning Electron

Transmission electron microscope(TEM) and scanning electron microscope(SEM) were widely used in experimental tumor studies. They are useful for evaluation of cellular transformation in vitro, classification of histological types of tumors and treating effect of tumors. We have obtained some results as follows:1. Studies on the malignant transformation of mammalian cells in vitro. Syrian golden hamster embryo cells(SGHEC) were transformed in vitro by ThO2 and/or ore dust. In a few days after dust added into medium, some dust crystals were phagocytized. Two weeks later, malignant transformation took place. These cells were of different size, nuclear pleomorphism, numerous ribosomes, increasing of microvilli on cell surface with various length and thickness, and blebs and ruffles(Figs. 1,2). Myelomonocytic leukemic transformation of mouse embryo cells(MEC) was induced in vitro by 3H-TdR. Transformed cells were become round from fusiform. The number of mitochondria and endoplasmic reticulum was reduced, ribosomes and nucleoli increased, shape of nuclei irregular, microvilli increased, and blebs and ruffles appeared(Fig. 3).

Clinicopathological study of primary malignant lymphoma of the large intestine in Japan

2001 ◽  
Vol 120 (5) ◽  
pp. A257-A257
Author(s):  
K KAMIYA ◽  
H GOTO ◽  
Y NIWA ◽  
N OHMIYA ◽  
T HAYAKAWA
Keyword(s):  
Malignant Lymphoma ◽  
Large Intestine ◽  
Clinicopathological Study

Malignant lymphoma occurring in patient and pet

JAMA ◽  
1966 ◽  
Vol 196 (1) ◽  
pp. 99-101 ◽  
Author(s):  
L. M. Drusin
Keyword(s):  
Malignant Lymphoma
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