Mouse Fetal Antigens in Rauscher Leukemia

2015 ◽  
pp. 311-317
Author(s):  
Akinori Ishimoto ◽  
Yumiko Suzuki ◽  
Toyoko Yoshida ◽  
Yohei Ito
Keyword(s):  
Rauscher Leukemia ◽  
Fetal Antigens

Electron Immunochemical Labeling of Ultrathin Sections of Murine Tumor Viruses and Cell Cultures using the Unlabeled Antibody Enzyme Technique

1976 ◽  
Vol 34 ◽  
pp. 266-267
Author(s):  
W. J. Hamilton
Keyword(s):  
Mammary Tumor ◽  
Leukemia Virus ◽  
Parallel Methods ◽  
Tumor Viruses ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Antibody Conjugates ◽  
Rauscher Leukemia ◽  
Antigen Localization ◽  
Unlabeled Antibody

The study of RNA tumor viruses has been greatly facilitated by the use of immunochemical tagging methods. In the past these methods have been constrained to antibody conjugates with ferritin or peroxidase. In order to avoid the disadvantages of using conjugated antisera, investigators have applied the unlabeled antibody enzyme method of Sternberger to mammary tumor derived mouse cells prior to embedding for electron microscopy. The current study has successfully applied the Sternberger method to virusproducing cells and purified virus pellets after epoxy-embedding and ultrathin sectioning. The results demonstrate the distinct advantages of this “post-embedding” method for viral antigen localization.Purified Rauscher leukemia virus (RLV) and mouse mammary tumor virus (MMTV) were pelleted, fixed in buffered 2% paraformaldehyde and washed thoroughly. These were dehydrated in acetone, infiltrated and embedded in Spurr resin according to common procedures. A tumor derived cell line, Mm5mt, producing MMTV was embedded by parallel methods.

scholarly journals Immune function during pregnancy varies between ecologically distinct populations

2020 ◽  
Vol 2020 (1) ◽  
pp. 114-128
Author(s):  
Carmen Hové ◽  
Benjamin C Trumble ◽  
Amy S Anderson ◽  
Jonathan Stieglitz ◽  
Hillard Kaplan ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Future Research ◽  
Differential Leukocyte Count ◽  
Ecological Conditions ◽  
Trade Offs ◽  
The Usa ◽  
Immunological Changes ◽  
Fetal Antigens ◽  
Pathogen Exposure

Abstract Background and objectives Among placental mammals, females undergo immunological shifts during pregnancy to accommodate the fetus (i.e. fetal tolerance). Fetal tolerance has primarily been characterized within post-industrial populations experiencing evolutionarily novel conditions (e.g. reduced pathogen exposure), which may shape maternal response to fetal antigens. This study investigates how ecological conditions affect maternal immune status during pregnancy by comparing the direction and magnitude of immunological changes associated with each trimester among the Tsimane (a subsistence population subjected to high pathogen load) and women in the USA. Methodology Data from the Tsimane Health and Life History Project (N = 935) and the National Health and Nutrition Examination Survey (N = 1395) were used to estimate population-specific effects of trimester on differential leukocyte count and C-reactive protein (CRP), a marker of systemic inflammation. Results In both populations, pregnancy was associated with increased neutrophil prevalence, reduced lymphocyte and eosinophil count and elevated CRP. Compared to their US counterparts, pregnant Tsimane women exhibited elevated lymphocyte and eosinophil counts, fewer neutrophils and monocytes and lower CRP. Total leukocyte count remained high and unchanged among pregnant Tsimane women while pregnant US women exhibited substantially elevated counts, resulting in overlapping leukocyte prevalence among all third-trimester individuals. Conclusions and implications Our findings indicate that ecological conditions shape non-pregnant immune baselines and the magnitude of immunological shifts during pregnancy via developmental constraints and current trade-offs. Future research should investigate how such flexibility impacts maternal health and disease susceptibility, particularly the degree to which chronic pathogen exposure might dampen inflammatory response to fetal antigens. Lay Summary This study compares immunological changes associated with pregnancy between the Tsimane (an Amazonian subsistence population) and individuals in the USA. Results suggest that while pregnancy enhances non-specific defenses and dampens both antigen-specific immunity and parasite/allergy response, ecological conditions strongly influence immune baselines and the magnitude of shifts during gestation.

The nucleotides at the RNA-DNA joint formed by the DNA polymerase of rauscher leukemia virus

Virology ◽  
1972 ◽  
Vol 50 (3) ◽  
pp. 935-938 ◽  
Author(s):  
Hiromi Okabe ◽  
Gerald G. Lovinger ◽  
Raymond V. Gilden ◽  
Masakazu Hatanaka
Keyword(s):  
Dna Polymerase ◽  
Leukemia Virus ◽  
Rauscher Leukemia ◽  
Rauscher Leukemia Virus

Propagation of Rauscher Leukemia Virus to Human Lymphoblastoid Cells by in Vivo Diffusion Chamber Cultivation

1973 ◽  
Vol 143 (3) ◽  
pp. 850-853
Author(s):  
I. Miyoshi ◽  
S. Yoshimoto ◽  
T. Tsubota ◽  
S. Fujiwara ◽  
H. Dabasaki ◽  
...  
Keyword(s):  
Leukemia Virus ◽  
Diffusion Chamber ◽  
Lymphoblastoid Cells ◽  
Rauscher Leukemia ◽  
Rauscher Leukemia Virus

Simultaneous purification of RNA-dependent DNA polymerase and gs-antigen from rauscher leukemia virus

1975 ◽  
Vol 63 (2) ◽  
pp. 400-408 ◽  
Author(s):  
Jyoti Deepak ◽  
Joann Comer ◽  
Michael Bowling ◽  
J. Dobbs ◽  
P.H. Aldenderfer ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Leukemia Virus ◽  
Rauscher Leukemia ◽  
Rauscher Leukemia Virus

scholarly journals ANTIGENIC PROPERTIES OF MURINE SARCOMA VIRUS-TRANSFORMED BALB/3T3 NONPRODUCER CELLS

1972 ◽  
Vol 135 (3) ◽  
pp. 503-515 ◽  
Author(s):  
John R. Stephenson ◽  
Stuart A. Aaronson
Keyword(s):  
Cell Line ◽  
Leukemia Virus ◽  
Target Cells ◽  
Subsequent Challenge ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Rauscher Leukemia ◽  
Transplantation Antigens ◽  
Murine Sarcoma ◽  
Rauscher Leukemia Virus

The isolation of clonal lines of murine sarcoma virus-transformed, non-producer BALB/3T3 cells has provided a model system for determining whether RNA tumor virus-transformed cells possess virus-specific transplantation antigens. MSV nonproducer cells (K-234) were clonally derived from an inbred mouse cell line, BALB/3T3. A parallel virus-producing cell line was obtained by infection of the MSV nonproducer cells with Rauscher leukemia virus. K-234 was much more tumorigenic than K-234(R). Preimmunization of syngeneic mice with either K-234(R) or with UV-inactivated Rauscher leukemia virus induced transplantation resistance to subsequent challenge with K-234(R), but not with K-234. In contrast, mice preimmunized with nonproducer cells were not made resistant to subsequent challenge with the homologous cells. Antisera prepared from mice immunized with K-234(R) were specifically cytotoxic and positive by fluorescent antibody staining for K-234(R) target cells, but not to either BALB/3T3 or K-234. The results show that MSV nonproducer cells lack detectable transplantation antigens and suggest that the transplantation resistance to the producing cells is attributable to maturing virus at the cell surface.

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