Differentiation of B Lymphocytes in Lineage Development and in the Immune Response

2015 ◽  
pp. 40-65
Author(s):  
Ulrich H�mmerling
Keyword(s):  
Immune Response ◽  
B Lymphocytes

CD5+ B lymphocytes secrete IL-10 rather than TGF-β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome

Autoimmunity ◽  
2019 ◽  
Vol 52 (1) ◽  
pp. 12-20 ◽  
Author(s):  
Manjun Zhao ◽  
Ningning Duan ◽  
Yi Wang ◽  
Hongli Zhu ◽  
Hong Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
B Lymphocytes ◽  
Haemolytic Anaemia ◽  
Autoimmune Haemolytic Anaemia ◽  
Evans Syndrome ◽  
Tgf Β1

Some aspects of the H-2 system, the major histocompatibility system in the mouse

1978 ◽  
Vol 202 (1146) ◽  
pp. 117-158 ◽  
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
B Lymphocytes ◽  
Complement System ◽  
Chromosome 17 ◽  
T Complex ◽  
Histocompatibility System ◽  
Humoral Antibodies ◽  
Functional Classes ◽  
The Complement System

The H-2 system is a complex of at least ten loci carried by chromosome 17 of the mouse. The loci can be divided into three or four functional classes concerned with the control and execution of immune response. The different classes are in some specific way involved in: 1. differentiation of bone-marrow derived (B) lymphocytes, leading to the production of humoral antibodies detectable by serological methods; 2. differentiation of thymus-derived (T) lymphocytes, leading to the production of killer cells capable of specifically attacking relevant targets; 3. regulation of immune response, both humoral and cellular (this response can be either enhanced or suppressed); 4. biosynthesis and activation of components of the complement system. On the same chromosome as the H-2 is the T/t complex, which controls embryonic and spermatozoal differentiation. An interesting relation between H-2 and T/t might exist between the complexes of loci.

scholarly journals Influence of Immunotherapy with Autologous Dendritic Cells on Innate and Adaptive Immune Response in Cancer

2013 ◽  
Vol 7 ◽  
pp. CMO.S12268 ◽  
Author(s):  
Bruna F. Matias ◽  
Tânia M. De Oliveira ◽  
Cláudia M. Rodrigues ◽  
Douglas R. Abdalla ◽  
Letícia Montes ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Immune Response ◽  
Dendritic Cell ◽  
B Lymphocytes ◽  
Dendritic Cell Vaccine ◽  
Cell Vaccine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Autologous Dendritic Cell ◽  
Tnf Α ◽  
Ifn Γ

The objective of this study was to evaluate some of the mechanisms involved in the activation of the immune system in patients with advanced-stage cancer (n = 7) who received an autologous dendritic cell vaccine. We examined the immune response mediated by macrophages (CD14+), natural killer cells (CD56+), and B lymphocytes (CD19+) by flow cytometry and assessed the expression of Th1 (IFN-γ, TNF-α, IL-2, and IL-12), Th2 (IL-4), and Treg (TGF-β) cytokines by flow cytometry and an enzyme-linked immunosorbent assay. The CD14+ TNF-α+ population was significantly increased ( P < 0.04) when patients received the vaccine; IL-2 expression in both NK cells and in B lymphocytes was increased after a transient initial increase showed a nearly significant decrease ( P < 0.07 and P < 0.06 respectively), whereas the CD19+ and CD56+ populations did not show significant changes. Dendritic cell-based immunotherapy led to increased secretion of IFN-γ and IL-12 and reduced secretion of TGF-β. In conclusion, it is likely that the autologous dendritic cell vaccine stimulated the immune cells from the peripheral blood of patients with cancer and generally increased the production of Th1 cytokines, which are related to immunomodulatory responses against cancer.

scholarly journals The VP6 Protein of Rotavirus Interacts with a Large Fraction of Human Naive B Cells via Surface Immunoglobulins

2004 ◽  
Vol 78 (22) ◽  
pp. 12489-12496 ◽  
Author(s):  
Nathalie Parez ◽  
Antoine Garbarg-Chenon ◽  
Cynthia Fourgeux ◽  
Françoise Le Deist ◽  
Annabelle Servant-Delmas ◽  
...  
Keyword(s):  
Immune Response ◽  
B Cells ◽  
B Lymphocytes ◽  
B Lymphocyte ◽  
Large Fraction ◽  
Memory Cell ◽  
Viral Gastroenteritis ◽  
Human B Cells ◽  
Surface Immunoglobulins ◽  
The Individual

ABSTRACT Immunity to human group A rotavirus (RV), a major cause of viral gastroenteritis in infants, involves B lymphocytes that provide RV-specific antibodies. Additionally, some arguments suggest that naive B cells could be implicated in the first steps of the immune response against RV. The aim of our study was to analyze the interaction of VP6 and VP7 RV capsid proteins with human B cells depending on the immune status of the individual, i.e., naive or RV experienced. For this purpose, a two-color virus-like particle flow cytometry assay was devised to evaluate the blood B-lymphocyte reactivity to VP6 and VP7 proteins from healthy RV-exposed adults, recently infected infants, and neonates at birth. Both VP6 and VP7 interactions with B cells were mediated by surface immunoglobulins and probably by their Fab portions. VP7-reactive B lymphocytes were mainly detected from RV-experienced patients and almost exclusively in the CD27-positive memory cell fraction. Conversely, VP6-reactive B lymphocytes were detected at similar and high frequencies in adult, infant, and neonate samples. In adult samples, VP6 reacted with about 2% of the CD27-negative (CD27neg) naive B cells. These results demonstrated that the VP6 RV protein interacted with a large fraction of naive B lymphocytes from both adults and neonates. We propose that naive B cell-VP6 interaction might influence the strength and quality of the acquired immune response and should be considered for elaborating RV vaccine strategies.

scholarly journals Characterization of B1-cells during experimental leukomogenesis

2020 ◽  
Vol 65 (1) ◽  
pp. 35-40
Author(s):  
I. Yu. Ezdakova ◽  
O. V. Kapustina ◽  
M. I. Gulyukin ◽  
T. V. Stepanova
Keyword(s):  
Immune Response ◽  
B Cells ◽  
B Lymphocytes ◽  
Early Period ◽  
Primary Immune Response ◽  
Functional Difference ◽  
Infected Cattle ◽  
Primary Target ◽  
B1 Cells

Background. Bovine leukemia causes a significant polyclonal expansion of CD5+ , IgM+ B lymphocytes, known as persistent lymphocytosis (PL), in approximately 30% of infected cattle. However, it is not yet clear what happens to this subpopulation of B cells in the early period of infection of animals.Purpose. Quantitative characterization of IgM+ and CD5+ B cells during the immune response, which can provide important information on the mechanisms of lymphocyte priming in BLV infection.Material and methods. The experiment used BLV-negative calves of black-motley breed at the age of 8 months (n = 11). Animals (n = 8) were intravenously injected with blood of a BLV-positive cow. Control calves (n = 3) were injected with saline. Studies were performed before and after infection on days 5, 7, 14, 21, 28 and 65 of the immune response. The determination of the number of B-lymphocytes in the blood was carried out by the method of immunoperoxidase staining based on monoclonal antibodies to IgM, CD5.Results. As a result of the studies, it was found that the level of CD5+ B cells increases on the 14th day of the primary immune response, characterized by polyclonal proliferation of CD5+ B cells, which are the primary target for BLV. Our research data confirm that in the lymphocytes of experimentally infected cattle, surface aggregation of IgM and CD5 molecules on B-lymphocytes is absent.Discussion. It is known that the wave-like nature of IgM synthesis, which was shown in previous studies, depends on a subpopulation of B1 cells. After 7 days of the immune response, IgM+ and CD5+ cells do not correlate, which shows their functional difference. The increase in CD5+ cells is probably not associated with B cells, but with T cells differentiating under the influence of the virus.Conclusions. A subset of B1 cells is the primary target of cattle leukemia virus. The 65th day of the immune response is characterized by the expansion of IgM+ B cells, a decrease in the number of CD5+ cells and a uniform distribution of receptors around the perimeter of the cells.

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