Preferential in vivo Localization of 125I-Labeled Antibody in a Carcinogen-Induced Syngeneic Rat Tumor

2015 ◽  
pp. 270-283 ◽  
Author(s):  
W. F. Bale ◽  
M. A. Contreras ◽  
M. J. Izzo ◽  
D. Della Penta ◽  
D. J. Buchsbaum
Keyword(s):  
Rat Tumor

In Vivo Follow-up of Rat Tumor Models with 2-Deoxy-2-[F-18]fluoro-d-glucose/Dual-Head Coincidence Gamma Camera Imaging

2005 ◽  
Vol 7 (3) ◽  
pp. 220-228 ◽  
Author(s):  
Jacques Monteil ◽  
Aurélie Dutour ◽  
Barbara Akla ◽  
Thierry Chianéa ◽  
Valérie Le Brun ◽  
...  
Keyword(s):  
Gamma Camera ◽  
Tumor Models ◽  
Gamma Camera Imaging ◽  
Camera Imaging ◽  
Rat Tumor

scholarly journals Neural Progenitor Cell Lines Inhibit Rat Tumor Growth in Vivo

2004 ◽  
Vol 64 (15) ◽  
pp. 5347-5354 ◽  
Author(s):  
Karin Staflin ◽  
Gabriella Honeth ◽  
Suzanne Kalliomäki ◽  
Christian Kjellman ◽  
Klaus Edvardsen ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Cell Lines ◽  
Progenitor Cell ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Rat Tumor

scholarly journals Chemopreventive and Therapeutic Efficacy of Cinnamomum zeylanicum L. Bark in Experimental Breast Carcinoma: Mechanistic In Vivo and In Vitro Analyses

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1399 ◽  
Author(s):  
Peter Kubatka ◽  
Martin Kello ◽  
Karol Kajo ◽  
Marek Samec ◽  
Karin Jasek ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Essential Oil ◽  
Caspase 3 ◽  
Methylation Status ◽  
Cinnamomum Zeylanicum ◽  
Dose Dependent ◽  
Rat Tumor ◽  
Mcf 7

Comprehensive oncology research suggests an important role of phytochemicals or whole plant foods in the modulation of signaling pathways associated with anticancer action. The goal of this study is to assess the anticancer activities of Cinnamomum zeylanicum L. using rat, mouse, and cell line breast carcinoma models. C. zeylanicum (as bark powder) was administered in the diet at two concentrations of 0.1% (w/w) and 1% (w/w) during the whole experiment in chemically induced rat mammary carcinomas and a syngeneic 4T1 mouse model. After autopsy, histopathological and molecular evaluations of mammary gland tumors in rodents were carried out. Moreover, in vitro analyses using MCF-7 and MDA-MB-231 cells were performed. The dominant metabolites present in the tested C. zeylanicum essential oil (with relative content over 1%) were cinnamaldehyde, cinnamaldehyde dimethyl acetal, cinnamyl acetate, eugenol, linalool, eucalyptol, limonene, o-cymol, and α-terpineol. The natural mixture of mentioned molecules demonstrated significant anticancer effects in our study. In the mouse model, C. zeylanicum at a higher dose (1%) significantly decreased tumor volume by 44% when compared to controls. In addition, treated tumors showed a significant dose-dependent decrease in mitotic activity index by 29% (0.1%) and 45.5% (1%) in comparison with the control group. In rats, C. zeylanicum in both doses significantly reduced the tumor incidence by 15.5% and non-significantly suppressed tumor frequency by more than 30% when compared to controls. An evaluation of the mechanism of anticancer action using valid oncological markers showed several positive changes after treatment with C. zeylanicum. Histopathological analysis of treated rat tumor specimens showed a significant decrease in the ratio of high-/low-grade carcinomas compared to controls. In treated rat carcinomas, we found caspase-3 and Bax expression increase. On the other hand, we observed a decrease in Bcl-2, Ki67, VEGF, and CD24 expressions and MDA levels. Assessment of epigenetic changes in rat tumor cells in vivo showed a significant decrease in lysine methylation status of H3K4m3 and H3K9m3 in the high-dose treated group, a dose-dependent increase in H4K16ac levels (H4K20m3 was not changed), down-regulations of miR21 and miR155 in low-dose cinnamon groups (miR22 and miR34a were not modulated), and significant reduction of the methylation status of two out of five gene promoters—ATM and TIMP3 (PITX2, RASSF1, PTEN promoters were not changed). In vitro study confirmed results of animal studies, in that the essential oil of C. zeylanicum displayed significant anticancer efficacy in MCF-7 and MDA-MB-231 cells (using MTS, BrdU, cell cycle, annexin V/PI, caspase-3/7, Bcl-2, PARP, and mitochondrial membrane potential analyses). As a conclusion, C. zeylanicum L. showed chemopreventive and therapeutic activities in animal breast carcinoma models that were also significantly confirmed by mechanistic evaluations in vitro and in vivo.

Bleomycin but not its derivatives inhibits the in vivo shedding of a rat tumor-associated antigen

1993 ◽  
Vol 4 (2) ◽  
pp. 281
Author(s):  
Mark Micallef ◽  
Toshiyuki Shibara ◽  
Masuo Hosokawa ◽  
Hiroshi Kobayashi
Keyword(s):  
Tumor Associated Antigen ◽  
Rat Tumor

Bleomycin but not its derivatives inhibits the in vivo shedding of a rat tumor-associated antigen

1992 ◽  
Vol 3 (6) ◽  
pp. 659-664
Author(s):  
Mark Micallef ◽  
Toshiyuki Shibata ◽  
Masuo Hosokawa ◽  
Hiroshi Kobayashi
Keyword(s):  
Tumor Associated Antigen ◽  
Rat Tumor
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