Electron Probe Determination of Water Reabsorption along Single Tubules of the Rat Kidney

2015 ◽  
pp. 155-161
Author(s):  
F. Spinelli ◽  
H. Wirz ◽  
G. Pehling ◽  
K. G. Carroll
Keyword(s):  
Electron Probe ◽  
Rat Kidney ◽  
Water Reabsorption ◽  
Single Tubules

Reduction of Potassium in Kidney Proximal Tubules to Aid in Electron Probe X-Ray Microanalysis of Calcium

1985 ◽  
Vol 43 ◽  
pp. 474-475
Author(s):  
A. LeFurgey ◽  
P. Ingram ◽  
L.J. Mandel
Keyword(s):  
Smooth Muscle ◽  
Proximal Tubule ◽  
Electron Probe ◽  
Clinical Applications ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Target Cells ◽  
X Ray ◽  
Freeze Dried

For quantitative determination of subcellular Ca distribution by electron probe x-ray microanalysis, decreasing (and/or eliminating) the K content of the cell maximizes the ability to accurately separate the overlapping K Kß and Ca Kα peaks in the x-ray spectra. For example, rubidium has been effectively substituted for potassium in smooth muscle cells, thus giving an improvement in calcium measurements. Ouabain, a cardiac glycoside widely used in experimental and clinical applications, inhibits Na-K ATPase at the cell membrane and thus alters the cytoplasmic ion (Na,K) content of target cells. In epithelial cells primarily involved in active transport, such as the proximal tubule of the rabbit kidney, ouabain rapidly (t1/2= 2 mins) causes a decrease2 in intracellular K, but does not change intracellular total or free Ca for up to 30 mins. In the present study we have taken advantage of this effect of ouabain to determine the mitochondrial and cytoplasmic Ca content in freeze-dried cryosections of kidney proximal tubule by electron probe x-ray microanalysis.

scholarly journals Identification of vasopressin and determination of its corticomedullary levels in rat kidney tissue

1984 ◽  
Vol 26 (6) ◽  
pp. 785-790 ◽  
Author(s):  
Kurakazu Shimizu ◽  
Akihide Nakao ◽  
Tatsuya Nonaka ◽  
Hiroshi Oka
Keyword(s):  
Rat Kidney ◽  
Kidney Tissue

scholarly journals Immunohistochemical determination of calbindin-d28k in the kidney of postnatal rats

2012 ◽  
Vol 49 (No. 9) ◽  
pp. 334-338 ◽  
Author(s):  
S. Timurkaan ◽  
B. G Tarakci
Keyword(s):  
Proximal Tubule ◽  
Intracellular Calcium ◽  
Rat Kidney ◽  
Renal Corpuscle ◽  
Specific Localization ◽  
Calbindin D28k ◽  
Collecting Tubules ◽  
Convoluted Tubules ◽  
Postnatal Rats

The localization of calbindin-D28k was examined in rat kidney at different stages of development. Calbindin was found specifically in the distal convoluted tubules (DCT). In the 10 day old rat kidney many cells apparently belonging to the collecting tubules were positive. In the neonatal nephrons there was also localization of calbindin-D28k to the limb of Henle, suggesting a difference in the regulation of intracellular calcium during maturation. Proximal tubule, renal corpuscle and macula densa gave no specific localization of calbindin-D28k.

Investigation of Urtite as an Analog of Waste Slag from a Plasma Shaff Furnace

1995 ◽  
Vol 412 ◽  
Author(s):  
S. A. Dmitriev ◽  
S. V. Stefanovsky
Keyword(s):  
Radioactive Waste ◽  
Electron Probe ◽  
Nepheline Syenite ◽  
Shaft Furnace ◽  
Alkali Feldspar ◽  
Chemical Compositions ◽  
Natural Analog ◽  
First Approximation ◽  
Geochemical Investigation

abstractMineralogical-geochemical investigation of a sample of nepheline syenite (urtite) as a natural analog of final radioactive waste form has been performed. The specimen of urtite consists of nepheline, alkali feldspar, pyroxene, sphene, apatite and minor magnetite and amphibole. As a first approximation, urtite simulates the mineral composition of waste slag produced in a plasma shaft furnace at SIA “Radon”. Determination of chemical compositions of the minerals by electron-probe microanalysis has shown that the main phases that hosted radionuclides and their geochemical analogs are as follows: nepheline (Rb and probably Cs), feldspar (Ba), sphene (Zr, Nb, REE, and actinides) and apatite (Sr, REE, and actinides).

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