The Effect of Castration and Peroral Estrogen Therapy on a Woman�s Skin

The Ultrastructure of Metastatic Human Mammary Carcinoma After Prolonged Estrogen Therapy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006091x ◽

1967 ◽

Vol 25 ◽

pp. 180-181

Author(s):

John H.L. Watson ◽

John L. Swedo ◽

R.W. Talley

Keyword(s):

Mammary Carcinoma ◽

Osmium Tetroxide ◽

Estrogen Therapy ◽

Ultrastructural Level ◽

Uranyl Acetate ◽

Human Mammary Carcinoma ◽

Surgical Biopsy ◽

Lead Hydroxide ◽

Preliminary Study ◽

Hormonal Therapies

A preliminary study of human mammary carcinoma on the ultrastructural level is reported for a metastatic, subcutaneous nodule, obtained as a surgical biopsy. The patient's tumor had responded favorably to a series of hormonal therapies, including androgens, estrogens, progestins, and corticoids for recurring nodules over eight years. The pertinent nodule was removed from the region of the gluteal maximus, two weeks following stilbestrol therapy. It was about 1.5 cms in diameter, and was located within the dermis. Pieces from it were fixed immediately in cold fixatives: phosphate buffered osmium tetroxide, glutaraldehyde, and paraformaldehyde. Embedment in each case was in Vestopal W. Contrasting was done with combinations of uranyl acetate and lead hydroxide.

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Further Ultrastructural Observations on Metastatic Nodules of Human Breast Cancer

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010010086x ◽

1968 ◽

Vol 26 ◽

pp. 224-225

Author(s):

John L. Swedo ◽

R. W. Talley ◽

John H. L. Watson

Keyword(s):

Breast Cancer ◽

Human Breast Cancer ◽

Estrogen Therapy ◽

Specimen Preparation ◽

Human Breast ◽

Autophagic Vacuoles ◽

Previous Communication ◽

Linear Profiles ◽

Metastatic Nodule

Since the report, which described the ultrastructure of a metastatic nodule of human breast cancer after estrogen therapy, additional ultrastructural observations, including some which are correlative with pertinent findings in the literature concerning mycoplasmas, have been recorded concerning the same subject. Specimen preparation was identical to that in.The mitochondria possessed few cristae, and were deteriorated and vacuolated. They often contained particulates and fibrous structures, sometimes arranged in spindle-shaped bundles, Fig. 1. Another apparent aberration was the occurrence, Fig. 2 (arrows) of linear profiles of what seems to be SER, which lie between layers of RER, and are often recognizably continuous with them.It was noted that the structure of the round bodies, interpreted as within autophagic vacuoles in the previous communication, and of vesicular bodies, described morphologically closely resembled those of some mycoplasmas. Specifically, they simulated or reflected the various stages of replication reported for mycoplasmas grown on solid nutrient. Based on this observation, they are referred to here as “mycoplasma-like” structures, in anticipation of confirmatory evidence from investigations now in progress.

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The Effects of Estradiol on Chronically Damaged Human Fallopian Tubes: A SEM Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119934 ◽

1985 ◽

Vol 43 ◽

pp. 648-649

Author(s):

A. Toledo ◽

G. Stoelk ◽

M. Yussman ◽

R.P. Apkarian

Keyword(s):

Menstrual Cycle ◽

Epithelial Cell ◽

Secretory Cell ◽

Estrogen Therapy ◽

Fallopian Tubes ◽

Cell Surfaces ◽

Proliferative Phase ◽

Cyclic Patterns ◽

Sem Study ◽

The U.S

Today it is estimated that one of every three women in the U.S. will have problems achieving pregnancy. 20-30% of these women will have some form of oviductal problems as the etiology of their infertility. Chronically damaged oviducts present problems with loss of both ciliary and microvillar epithelial cell surfaces. Estradiol is known to influence cyclic patterns in secretory cell microvilli and tubal ciliogenesis, The purpose of this study was to assess whether estrogen therapy could stimulate ciliogenesis in chronically damaged human fallopian tubes.Tissues from large hydrosalpinges were obtained from six women undergoing tuboplastic repair while in the early proliferative phase of fheir menstrual cycle. In each case the damaged tissue was rinsed in heparinized Ringers-lactate and quartered.

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Female sex steroid induced gallbladder epithelium changes and gallstone deposits in female hamsters: TEM and SEM aspects

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147612 ◽

1993 ◽

Vol 51 ◽

pp. 354-355

Author(s):

S. Karkare ◽

J. Gilloteaux ◽

T. R. Kelly

Keyword(s):

Hormone Receptors ◽

Corn Oil ◽

Estrogen Therapy ◽

Gallstone Formation ◽

The United States ◽

Sex Steroid ◽

Estradiol Valerate ◽

Surface Epithelium ◽

Female Sex ◽

Purina Chow

Approximately 1 million people in the United States alone develop gallstones each year. The incidence is higher in women than in men and the ratio being 4 ≥ 1. A correlation has also been suggested between oral contraceptives and cholelithiasis. In addition, postmenopausal or cancer estrogen therapy has been reported to be a factor responsible for gallstone formation. Female sex hormone receptors have been detected not only in the gallbladder musculature, but also in its epithelium. As a follow up to experiments effectuated in the male and the ovariectomized Syrian hamster, this report shows that, a combination of a low cholesterol diet with female sex steroid treatment contributes to the formation of gallstone-like deposits, while modifying the surface epithelium morphology. Syrian hamsters (F1B strain, BioBreeders, Watertown MA) were housed under 12h light: 12 h dark cycle, at 20 °C, fed Purina chow and water ad libitum. Several duration/treatment groups were studied, but this report will focus on data obtained with the group injected weekly with estradiol valerate (E weekly, s.c. 8-10 μg/100 g.b.w., in corn oil) and with i.m. medroxyprogesterone acetate (DepoProvera Upjohn Co., Kalamazoo, MI; 8-10 mg/100 g.b.w.) for a 3-month period. Other parameters (blood and bile) were also studied but not reported here.

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