Development of Preparative Scale High Performance Liquid Chromatography for Peptide and Protein Purification

2015 ◽  
pp. 195-206
Author(s):  
Thomas F. Gabriel ◽  
Joseph Michalewsky ◽  
Johannes Meienhofer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Protein Purification ◽  
High Performance ◽  
Preparative Scale

scholarly journals Isolation of Chavibetol and Methyleugenol from Essential Oil of Pimenta pseudocaryophyllus by High Performance Liquid Chromatography

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2909 ◽  
Author(s):  
Edenilson Niculau ◽  
Leandro Ribeiro ◽  
Thiago Ansante ◽  
João Fernandes ◽  
Moacir Forim ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Spectral Analysis ◽  
Liquid Chromatography ◽  
Essential Oil ◽  
High Purity ◽  
High Performance ◽  
Insect Pests ◽  
Hplc Method ◽  
Preparative Scale ◽  
Mass Recovery

A high performance liquid chromatography (HPLC) method was developed for the simultaneous isolation, on a semi-preparative scale, of chavibetol and methyleugenol from the crude essential oil of P. pseudocaryophyllus leaves. The purity of the isolated compounds and their quantifications were developed using GC/FID. Chavibetol was isolated with high purity (98.7%) and mass recovery (94.6%). The mass recovery (86.4%) and purity (85.3%) of methyleugenol were lower than those of chavibetol. Both compounds were identified on the basis of spectral analysis. The results suggest that the method can provide chavibetol with high purity, mass recovery, and productivity from crude essential, which will be used in bioassays against stored insect pests.

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