Single-cell sequencing technology in tumor research

2021 ◽  
Author(s):  
Xue Bai ◽  
Yuxuan Li ◽  
Xuemei Zeng ◽  
Qiang Zhao ◽  
Zhiwei Zhang
Keyword(s):  
Single Cell ◽  
Sequencing Technology ◽  
Single Cell Sequencing ◽  
Tumor Research

scholarly journals CRISPR/Cas9 high-throughput screening in cancer research

2020 ◽  
Vol 185 ◽  
pp. 03032
Author(s):  
Zhuoxin Liu
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Genomic Library ◽  
Gene Activation ◽  
Library Screening ◽  
Basic Principles ◽  
Tumor Research ◽  
Technology Strategies ◽  
Target Effect ◽  
Main Strategy

In recent years, CRISPR/Cas9 technology has developed rapidly. With its accurate, fast, and simple editing functions that can achieve gene activation, interference, knockout, and knock-in, it has become a powerful genetic screening tool that is widely used in various models, including cell lines of mice and zebrafish. The use of CRISPR system to construct a genomic library for high-throughput screening is the main strategy for research of disease, especially tumor target gene research. This article reviews the basic principles and latest developments of CRISPR/Cas9 library screening technology strategies to improve its off-target effect, the basic workflow of library screening, and its application in tumor research.

scholarly journals GE-11 * THE FLORIDA CENTER FOR BRAIN TUMOR RESEARCH (FCBTR): BUILDING INFRASTRUCTURE TO FIND A CURE

2014 ◽  
Vol 16 (suppl 5) ◽  
pp. v98-v98
Author(s):  
B. Frentzen, ◽  
D. Mitchell, ◽  
W. A. Friedman ◽  
A. Yachnis ◽  
R. McTiernan
Keyword(s):  
Brain Tumor ◽  
Tumor Research ◽  
Brain Tumor Research
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