The Chemical Assay of Ergot

BIO-CHEMICAL ASSAY OF PHOTOSYNTHETIC PIGMENTS IN BLACK GRAM (VIGNA MUNGO L.) EXPOSED TO CEMENT DUST POLLUTION

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE ◽

10.32395/ijprs.v7.i4.00001 ◽

2018 ◽

Vol 7 (4) ◽

pp. 1-7

Author(s):

RAAJASUBRAMANIAN DEVARAJAN ◽

K. NARENDRA ◽

NAVYA KRISHNARAM ◽

KRISHNA RAM HANUMAPPA

Keyword(s):

Photosynthetic Pigments ◽

Vigna Mungo ◽

Black Gram ◽

Cement Dust ◽

Dust Pollution ◽

Chemical Assay ◽

Cement Dust Pollution

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COMPARISON OF VARIOUS METHODS FOR THE CHEMICAL ASSAY OF CORTICOIDS IN URINE

Acta Endocrinologica ◽

10.1530/acta.0.0180374 ◽

1955 ◽

Vol 18 (4) ◽

pp. 374-378

Author(s):

Mogens Sprechler

Keyword(s):

Calibration Curve ◽

Periodic Acid ◽

Reducing Power ◽

Standard Technique ◽

Phosphomolybdic Acid ◽

Florisil Column ◽

Chemical Assay ◽

Chromatographic Procedure ◽

Acid Reagent

SUMMARY Since 1949 about 10,000 urinary corticoid analyses have been performed routinely in our laboratory. The method used for this purpose was described in 1950 (Sprechler). We determine the corticoids which can be extracted from the urine with chloroform immediately after acidification to pH 1. The extract is washed with sodium hydroxide and water, a Girard separation is performed, and finally the reducing power of the ketonic fraction is measured by means of the phosphomolybdic acid reagent reaction. During the last few years two other chemical reactions have been used for comparison: The formaldehyde and the Porter-Silber method. After a thorough examination of the above methods a standard technique was followed. In the formaldehyde method a microdiffusion in a Conway unit was used instead of distillation of the formaldehyde following the oxidation with periodic acid. The calibration curve was corrected for loss of material by taking the standard doses of DOC through all the procedures of the method. A micromodification of the Porter-Silber method was chosen. Furthermore attempts were made to determine how specific the chromatographic procedure is in the determination of steroids in urinary extracts. For this purpose the Florisil column was used, and the technique described by Nelson & Samuels was followed. Finally we have investigated the glucuronide-bound corticoids in urine in a smaller series of objects.

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