Chapter XIV Anaemias Due or Probably Due to Humoral Antibodies, Drugs and Poisons

2015 ◽  
pp. 246-368
Author(s):  
H. S. Baar
Keyword(s):  
Humoral Antibodies

Humoral antibodies in renal homograft

JAMA ◽  
1966 ◽  
Vol 198 (3) ◽  
pp. 226-230 ◽  
Author(s):  
F. Milgrom
Keyword(s):  
Humoral Antibodies ◽  
Renal Homograft

scholarly journals Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 281
Author(s):  
Isaías Sanmartín ◽  
Luis Sendra ◽  
Inés Moret ◽  
María José Herrero ◽  
Salvador F. Aliño
Keyword(s):  
Immune Response ◽  
Humoral Response ◽  
Water Soluble ◽  
Vaccine Research ◽  
Current Vaccine ◽  
Humoral Antibodies ◽  
Melanoma Model ◽  
Gd3 Ganglioside ◽  
Lipid Antigen ◽  
Simultaneous Delivery

Vector design and its characterization is an area of great interest in current vaccine research. In this article, we have formulated and characterized a multicompartmental lipopolyplex, which associates multiple liposomes and polyplexes in the same complex. These particles allow the simultaneous delivery of lipid or water-soluble antigens associated with genes to the same cell, in much higher amounts than conventional lipopolyplexes. The vector characterization and optimization were carried out using liposomes with entrapped carboxyfluorescein and adapted electrophoretic assays. Two types of lipopolyplexes (containing hydrophilic or lipophilic antigens) were employed to evaluate their interest in vaccination. The lipopolyplex loaded with an extract of water-soluble melanoma proteins proved to efficiently induce humoral response in murine melanoma model, increasing the levels of IgM and IgG. The specificity of the immune response induced by the lipopolyplex was demonstrated in mice with the lipopolyplex containing the GD3 ganglioside lipid antigen, abundant in melanoma cells. The levels of anti-GD3 IgG increased markedly without modifying the expression of humoral antibodies against other gangliosides.

Humoral Antibodies Against Skin Allografts

1979 ◽  
Vol 13 (1) ◽  
pp. 77-78
Author(s):  
L. Pávková ◽  
Z. Koničková ◽  
R. Königová ◽  
R. Vrabec
Keyword(s):  
Skin Allografts ◽  
Humoral Antibodies

scholarly journals Generation of Lamprey Monoclonal Antibodies (Lampribodies) Using the Phage Display System

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 868 ◽  
Author(s):  
Khan M. A. Hassan ◽  
John D. Hansen ◽  
Brantley R. Herrin ◽  
Chris T. Amemiya
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Genomic Rearrangements ◽  
Filamentous Phage ◽  
Human Cell Lines ◽  
Display System ◽  
Phage Display Libraries ◽  
Humoral Antibodies ◽  
Leucine Rich Repeats ◽  
Lymphocyte Receptors

The variable lymphocyte receptors (VLRs) consist of leucine rich repeats (LRRs) and comprise the humoral antibodies produced by lampreys and hagfishes. The diversity of the molecules is generated by stepwise genomic rearrangements of LRR cassettes dispersed throughout the VLRB locus. Previously, target-specific monovalent VLRB antibodies were isolated from sea lamprey larvae after immunization with model antigens. Further, the cloned VLR cDNAs from activated lamprey leukocytes were transfected into human cell lines or yeast to select best binders. Here, we expand on the overall utility of the VLRB technology by introducing it into a filamentous phage display system. We first tested the efficacy of isolating phage into which known VLRB molecules were cloned after a series of dilutions. These experiments showed that targeted VLRB clones could easily be recovered even after extensive dilutions (1 to 109). We further utilized the system to isolate target-specific “lampribodies” from phage display libraries from immunized animals and observed an amplification of binders with relative high affinities by competitive binding. The lampribodies can be individually purified and ostensibly utilized for applications for which conventional monoclonal antibodies are employed.

Humoral Antibodies in Renal Allotransplantation in Man

1970 ◽  
Vol 282 (3) ◽  
pp. 111-117 ◽  
Author(s):  
M. Jeannet ◽  
V. W. Pinn ◽  
M. H. Flax ◽  
H. J. Winn ◽  
P. S. Russell
Keyword(s):  
Humoral Antibodies
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