Properties of Mitochondrial Preparations from Rabbit Lung

2015 ◽  
pp. 100-103
Author(s):  
O. K. Reiss
Keyword(s):  
Rabbit Lung

The Effect of Several Tissue Thromboplastins on the Activation of the Abnormal Factor X (Factor X Friuli)

1972 ◽  
Vol 27 (03) ◽  
pp. 535-542 ◽  
Author(s):  
A Girolami ◽  
M Lazzarin ◽  
G Molaro
Keyword(s):  
Prothrombin Time ◽  
Rabbit Brain ◽  
Factor X ◽  
Dilution Curve ◽  
Human Origin ◽  
Rabbit Lung ◽  
Normal Ratio

SummaryThe effect of several tissue thromboplastins on the abnormal factor X (factor X Friuli) has been investigated.The prothrombin time varied between 33.6 and 69 sec. The prothrombin time percentile values (saline dilution curve and Quick’s formula for citrated plasma) varied between 6.6 and 22% and between 10.9 and 32.8%, respectively. The prothrombin time patient/normal ratio varied between 2.24 and 4.43.The factor X level varied between 3.5 and 20% of normal.Significant correlations were found to exist between the percentile factor X level and the prothrombin time in seconds, the percentile prothrombin time values and the prothrombin time patient/normal ratio. Thromboplastins of human origin yielded the lowest factor X values namely 5% thereby appearing to be practically “inert” with regard to the abnormal factor X. Thromboplastins obtained from rabbit lung on the contrary yielded the highest values, namely 15.3%. Thromboplastins obtained from simian or rabbit brain gave values intermediate between these two extremes.

Soluble Thrombomodulin Purified from Human Urine Exhibits a Potent Anticoagulant Effect In Vitro and In Vivo

1995 ◽  
Vol 73 (05) ◽  
pp. 805-811 ◽  
Author(s):  
Yasuo Takahashi ◽  
Yoshitaka Hosaka ◽  
Hiromi Niina ◽  
Katsuaki Nagasawa ◽  
Masaaki Naotsuka ◽  
...  
Keyword(s):  
Human Urine ◽  
Specific Activity ◽  
Anticoagulant Activity ◽  
Rabbit Lung ◽  
Soluble Thrombomodulin ◽  
Molecular Weights ◽  
Dose Dependent Fashion ◽  
Dose Dependent

SummaryWe examined the anticoagulant activity of two major molecules of soluble thrombomodulin purified from human urine. The apparent molecular weights of these urinary thrombomodulins (UTMs) were 72,000 and 79,000, respectively. Both UTMs showed more potent cofactor activity for protein C activation [specific activity >5,000 thrombomodulin units (TMU)/mg] than human placental thrombomodulin (2,180 TMU/mg) and rabbit lung thrombomodulin (1,980 TMU/mg). The UTMs prolonged thrombin-induced fibrinogen clotting time (>1 TMU/ml), APTT (>5 TMU/ml), TT (>5 TMU/ml) and PT (>40 TMU/ml) in a dose-dependent fashion. These effects appeared in the concentration range of soluble thrombomodulins present in human plasma and urine. In the rat DIC model induced by thromboplastin, administration of UTMs by infusion (300-3,000 TMU/kg) restored the hematological abnormalities derived from DIC in a dose-dependent fashion. These results demonstrate that UTMs exhibit potent anticoagulant and antithrombotic activities, and could play a physiologically important role in microcirculation.

Use of Different Tissue Thromboplastins in the Control of Anticoagulant-Therapy

1958 ◽  
Vol 02 (05/06) ◽  
pp. 462-480 ◽  
Author(s):  
Marc Verstraete ◽  
Patricia A. Clark ◽  
Irving S. Wright
Keyword(s):  
Prothrombin Time ◽  
Anticoagulant Therapy ◽  
Factor Vii ◽  
Rabbit Brain ◽  
Minor Role ◽  
Rabbit Lung ◽  
Coagulation Mechanism ◽  
Time Test ◽  
The One ◽  
A Minor

SummaryAn analysis of the results of prothrombin time tests with different types of thromboplastins sheds some light on the problem why the administration of coumarin is difficult to standardize in different centers. Our present ideas on the subject, based on experimental data may be summarized as follows.Several factors of the clotting mechanism are influenced by coumarin derivatives. The action of some of these factors is by-passed in the 1-stage prothrombin time test. The decrease of the prothrombin and factor VII levels may be evaluated in the 1-stage prothrombin time determination (Quick-test). The prolongation of the prothrombin times are, however, predominantly due to the decrease of factor VII activity, the prothrombin content remaining around 50 per cent of normal during an adequate anticoagulant therapy. It is unlikely that this degree of depression of prothrombin is of major significance in interfering with the coagulation mechanism in the protection against thromboembolism. It may, however, play a minor role, which has yet to be evaluated quantitatively. An exact evaluation of factor VII is, therefore, important for the guidance of anticoagulant therapy and the method of choice is the one which is most sensitive to changes in factor VII concentration. The 1-stage prothrombin time test with a rabbit lung thromboplastin seems the most suitable method because rabbit brain preparations exhibit a factor VII-like activity that is not present in rabbit lung preparations.

scholarly journals In vivo tumour imaging employing regional delivery of novel gallium radiolabelled polymer composites

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Ross W. Stephens ◽  
Gregory D. Tredwell ◽  
Jessica L. Bell ◽  
Karen J. Knox ◽  
Lee A. Philip ◽  
...  
Keyword(s):  
Normal Liver ◽  
Ct Imaging ◽  
Liver Imaging ◽  
Polymer Microspheres ◽  
Tumour Imaging ◽  
Planar Imaging ◽  
Rabbit Lung ◽  
Vitro Binding

Abstract Background Understanding the regional vascular delivery of particles to tumour sites is a prerequisite for developing new diagnostic and therapeutic composites for treatment of oncology patients. We describe a novel imageable 67Ga-radiolabelled polymer composite that is biocompatible in an animal tumour model and can be used for preclinical imaging investigations of the transit of different sized particles through arterial networks of normal and tumour-bearing organs. Results Radiolabelling of polymer microspheres with 67Ga was achieved using a simple mix and wash method, with tannic acid as an immobilising agent. Final in vitro binding yields after autoclaving averaged 94.7%. In vivo stability of the composite was demonstrated in New Zealand white rabbits by intravenous administration, and intrahepatic artery instillations were made in normal and VX2 tumour implanted rabbit livers. Stability of radiolabel was sufficient for rabbit lung and liver imaging over at least 3 hours and 1 hour respectively, with lung retention of radiolabel over 91%, and retention in both normal and VX2 implanted livers of over 95%. SPECT-CT imaging of anaesthetised animals and planar imaging of excised livers showed visible accumulation of radiolabel in tumours. Importantly, microsphere administration and complete liver dispersal was more easily achieved with 8 μm diameter MS than with 30 μm MS, and the smaller microspheres provided more distinct and localised tumour imaging. Conclusion This method of producing 67Ga-radiolabelled polymer microspheres is suitable for SPECT-CT imaging of the regional vascular delivery of microspheres to tumour sites in animal models. Sharper distinction of model tumours from normal liver was obtained with smaller MS, and tumour resolution may be further improved by the use of 68Ga instead of 67Ga, to enable PET imaging.

scholarly journals Quantification of surfactant pool sizes in rabbit lung during perinatal development.

1988 ◽  
Vol 27 (6) ◽  
pp. 602-612
Author(s):  
M Oulton ◽  
M Fraser ◽  
M Dolphin ◽  
R Yoon ◽  
G Faulkner
Keyword(s):  
Rabbit Lung ◽  
Perinatal Development ◽  
Pool Sizes
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