Purification of Bacterial Thiaminase I and its Properties

2015 ◽  
pp. 116-125
Author(s):  
Kiku Murata ◽  
J. Ebata
Keyword(s):  
Thiaminase I

scholarly journals The Action of Bacterial Thiaminase I on Thiamine

1968 ◽  
Vol 14 (1) ◽  
pp. 12-20 ◽  
Author(s):  
KIKU MURATA ◽  
JUNKO EBATA ◽  
MITSUKO SOMEKAWA ◽  
SUMIKO MARUKAWA
Keyword(s):  
Thiaminase I

Thiaminase I of Bacillus thiaminolyticus

1966 ◽  
Vol 113 (2) ◽  
pp. 331-337 ◽  
Author(s):  
H.A. Douthit ◽  
R.L. Airth
Keyword(s):  
Thiaminase I

Abstract 35: Metabolomic analysis of MCF-7 and MCF-10 cells after thiaminase I exposure

2010 ◽  
Author(s):  
Adam D. Richardson ◽  
Shuqian Liu ◽  
Jeffrey A. Moscow
Keyword(s):  
Metabolomic Analysis ◽  
Thiaminase I ◽  
Mcf 7

Studies on thiaminase I activity in ruminant faeces and rumen bacteria

1985 ◽  
Vol 104 (3) ◽  
pp. 637-642 ◽  
Author(s):  
J. W. Boyd
Keyword(s):  
Radioactive Isotope ◽  
Colorimetric Method ◽  
Kinetic Studies ◽  
Rumen Bacteria ◽  
Michaelis Constant ◽  
Thiaminase I ◽  
Alternate Substrate ◽  
Reaction Constants ◽  
Analytical Variation ◽  
Substrate Concentrations

SummaryKinetic studies of thiaminase I in extracts of ruminant faeces showed that the affinity for one substrate varied with the concentration of the other substrate in the manner of a two-step transfer mechanism. When the alternate substrate concentration was optimal, the apparent Michaelis constant (Km) for thiamine was 176 μΜ and the apparent Km for aniline was 3·19 mΜ. It is recommended that in routine thiaminase assays, the thiamine and aniline concentrations should be at least 1·5 and 25 mΜ, respectively. When non-saturating concentrations of thiamine are used in thiaminase assays the results should be reported as unimolecular reaction constants since the enzyme activity can be calculated only if suitable Km data have been determined.Improved radioactive and colorimetric thiaminase assays with saturating substrate concentrations gave similar results. The analytical variation of the colorimetric method was rather high but this method may be useful for laboratories which lack radioactive isotope facilities.Thiaminase assays were performed on cultures of 14 species of rumen bacteria. Only Megasphaera elsdenii had thiaminase activity and its cosubstrate specificity was different from the rumen thiaminase associated with cerebrocortical necrosis in ruminants. It was concluded that the source of rumen thiaminase in that disease has yet to be identified.

scholarly journals Mechanistic Studies on Thiaminase I

1996 ◽  
Vol 271 (7) ◽  
pp. 3445-3452 ◽  
Author(s):  
Colleen A. Costello ◽  
Neil L. Kelleher ◽  
Mitsuko Abe ◽  
Fred W. McLafferty ◽  
Tadhg P. Begley
Keyword(s):  
Mechanistic Studies ◽  
Thiaminase I

scholarly journals A Rapid Method for Assaying Thiaminase I Activity in Diverse Biological Samples

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e92688 ◽  
Author(s):  
Clifford E. Kraft ◽  
Eric R. L. Gordon ◽  
Esther R. Angert
Keyword(s):  
Rapid Method ◽  
Biological Samples ◽  
Thiaminase I

Paenibacillus thiaminolyticus is not the cause of thiamine deficiency impeding lake trout (Salvelinus namaycush) recruitment in the Great Lakes

2012 ◽  
Vol 69 (6) ◽  
pp. 1056-1064 ◽  
Author(s):  
Catherine A. Richter ◽  
Allison N. Evans ◽  
Maureen K. Wright-Osment ◽  
James L. Zajicek ◽  
Scott A. Heppell ◽  
...  
Keyword(s):  
Great Lakes ◽  
Thiamine Deficiency ◽  
Lake Trout ◽  
Bacterial Species ◽  
Primary Source ◽  
Salvelinus Namaycush ◽  
Trophic Levels ◽  
Thiaminase I ◽  
Global Concern ◽  
Paenibacillus Thiaminolyticus

Thiamine (vitamin B1) deficiency is a global concern affecting wildlife, livestock, and humans. In Great Lakes salmonines, thiamine deficiency causes embryo mortality and is an impediment to restoration of native lake trout ( Salvelinus namaycush ) stocks. Thiamine deficiency in fish may result from a diet of prey with high levels of thiaminase I. The discoveries that the bacterial species Paenibacillus thiaminolyticus produces thiaminase I, is found in viscera of thiaminase-containing prey fish, and causes mortality when fed to lake trout in the laboratory provided circumstantial evidence implicating P. thiaminolyticus. This study quantified the contribution of P. thiaminolyticus to the total thiaminase I activity in multiple trophic levels of Great Lakes food webs. Unexpectedly, no relationship between thiaminase activity and either the amount of P. thiaminolyticus thiaminase I protein or the abundance of P. thiaminolyticus cells was found. These results demonstrate that P. thiaminolyticus is not the primary source of thiaminase activity affecting Great Lakes salmonines and calls into question the long-standing assumption that P. thiaminolyticus is the source of thiaminase in other wild and domestic animals.

scholarly journals Studies on Thiaminase I in Marine Fish-IV

1974 ◽  
Vol 40 (7) ◽  
pp. 671-674 ◽  
Author(s):  
Tadashi ISHIHARA ◽  
Masato YASUDA
Keyword(s):  
Marine Fish ◽  
Thiaminase I

Quantitative Polymerase Chain Reaction (PCR) Assays for a Bacterial Thiaminase I Gene and the Thiaminase-Producing BacteriumPaenibacillus thiaminolyticus

2009 ◽  
Vol 21 (4) ◽  
pp. 229-238 ◽  
Author(s):  
Catherine A. Richter ◽  
Maureen K. Wright-Osment ◽  
James L. Zajicek ◽  
Dale C. Honeyfield ◽  
Donald E. Tillitt
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Thiaminase I ◽  
Polymerase Chain ◽  
Pcr Assays ◽  
I Gene
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