Diuresis, Inulin Clearance, Sodium Reabsorption and Oxygen Consumption of the Isolated Erythrocyte-Free Perfused Rabbit Kidney1

2015 ◽  
pp. 404-407
Author(s):  
W. Herms ◽  
G. Arnold
Keyword(s):  
Oxygen Consumption ◽  
Inulin Clearance ◽  
Sodium Reabsorption

scholarly journals Regulation of erythropoietin production is related to proximal tubular function

1989 ◽  
Vol 256 (5) ◽  
pp. F942-F947 ◽  
Author(s):  
K. U. Eckardt ◽  
A. Kurtz ◽  
C. Bauer
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Sensor ◽  
Collecting Duct ◽  
Inhibitory Effect ◽  
Tubular Function ◽  
Sodium Reabsorption ◽  
Renal Oxygen Consumption ◽  
Proximal Tubular ◽  
Proximal Tubular Function ◽  
Renal Oxygen

Regulation of renal erythropoietin (EPO) production is based on an intrarenal oxygen sensor. Whereas the sensitivity of this oxygen sensor to variations in renal oxygen supply is well established, the influence of changes in renal oxygen consumption has not yet been elucidated. Diuretic drugs, which inhibit active sodium reabsorption, reduce tubular oxygen consumption. We therefore investigated the effects of acetazolamide, furosemide, hydrochlorothiazide, and amiloride, known to preferentially inhibit sodium reabsorption at different segments of the nephron, on hypoxia-induced EPO formation in mice. Those drugs that are considered to act mainly in the loop of Henle, distal tubule, and collecting duct (furosemide, hydrochlorothiazide, and amiloride) did not impair EPO formation. Acetazolamide on the other hand, which is thought to act predominantly at the proximal tubular site, significantly reduced EPO formation in response to normobaric hypoxia (8 and 14% O2) and functional anemia (0.1% carbon monoxide). This inhibitory effect of acetazolamide was dose dependent and correlated with the natriuresis induced. It appeared not to depend on the metabolic acidosis induced by the drug, since the simultaneous administration of sodium bicarbonate, which restored standard bicarbonate levels to normal, did not diminish the inhibitory effect of acetazolamide on EPO production. In conclusion the data suggest that the regulation of EPO production is likely to be related to proximal tubular function.

Effects of cyanide, Q0, and dinitrophenol on renal sodium reabsorption and oxygen consumption

1964 ◽  
Vol 206 (6) ◽  
pp. 1327-1332 ◽  
Author(s):  
M. Fujimoto ◽  
F. D. Nash ◽  
R. H. Kessler
Keyword(s):  
Oxygen Consumption ◽  
Total Dose ◽  
Coenzyme Q ◽  
Atp Synthesis ◽  
Metabolic Inhibitors ◽  
Sodium Reabsorption ◽  
Major Fraction ◽  
Variable Effect ◽  
Anesthetized Dogs ◽  
Renal Sodium Reabsorption

Effects on sodium reabsorption and oxygen consumption of the renal arterial injection of three metabolic inhibitors were studied by unilateral clearance techniques in anesthetized dogs. In control studies, 21.5 ± 2.3 mEq sodium were reabsorbed per millimole oxygen consumed within a range of sodium reabsorption of from 2 to 9 mEq/min. A total dose of 10–4 m cyanide depressed both sodium reabsorption and oxygen consumption in the injected kidney. Administration of 10–4 m Q0, the quinone nucleus of coenzyme Q, reduced sodium reabsorption and had a variable effect on O2 consumption. Dinitrophenol, in a total dose of 10–3 m, increased oxygen consumption without affecting the per cent of filtered sodium that was reabsorbed. The resultant Na:O2 ratios were 12:1. We conclude that the major fraction of O2 consumption energizes sodium reabsorption in the kidney perhaps via the classic route of ATP synthesis and hydrolysis. However, all three compounds used in this study would be predicted to decrease renal ATP concentrations. Yet only cyanide and Q0 decreased sodium reabsorption. Energy for sodium movement may come directly from oxidative metabolism bypassing synthesis and breakdown of ATP.

Cholinergic stimulation of ouabain-sensitive respiration in rat submandibular gland

1983 ◽  
Vol 245 (3) ◽  
pp. G364-G368 ◽  
Author(s):  
D. J. Stewart ◽  
D. J. Pon ◽  
A. K. Sen
Keyword(s):  
Oxygen Consumption ◽  
Submandibular Gland ◽  
Sodium Pump ◽  
Calcium Ionophore ◽  
Sodium Reabsorption ◽  
Tissue Respiration ◽  
Ionophore A23187 ◽  
Cholinergic Stimulation ◽  
Rat Submandibular Gland ◽  
Stimulation Of

Oxygen consumption of slices of rat submandibular gland was monitored with an oxygen electrode method. Carbachol stimulated an immediate increase in tissue respiration that was inhibitable by ouabain. The stimulation required the presence of calcium in the incubation medium and was blocked by atropine. The calcium ionophore A23187 also stimulated ouabain-sensitive oxygen consumption in the tissue slices. The results show that the mechanism using the extra energy during cholinergic stimulation is the sodium pump. Amiloride at a 1, 10, or 100 microM concentration had no effect on stimulation of ouabain-sensitive respiration by carbachol. Since amiloride, which is known to block the sodium reabsorption process in the ductal segment, has no effect on the stimulation, the increased sodium pump activity is probably located in the acinar region and is associated with the primary fluid secretion process.

Renal sodium transport and oxygen consumption

1961 ◽  
Vol 201 (3) ◽  
pp. 511-516 ◽  
Author(s):  
Fredrik Kiil ◽  
Knut Aukland ◽  
Harald E. Refsum
Keyword(s):  
Oxygen Consumption ◽  
Glomerular Filtration Rate ◽  
Glomerular Filtration ◽  
Sodium Transport ◽  
Filtration Rate ◽  
Sodium Reabsorption ◽  
Renal Oxygen Consumption ◽  
Tubular Lumen ◽  
Oxygen Difference ◽  
Renal Oxygen

Glomerular filtration rate and renal blood flow were increased in dogs by infusion of 2% glycine and hypertonic NaCl at rates of 8 ml/min. This procedure resulted both in an increased rate of sodium reabsorption and in increased oxygen consumption. Approximately six equivalents of sodium were transported per equivalent oxygen consumed, a ratio similar to that obtained by others when glomerular filtration rate had been reduced. These observations strongly suggest that a large part of renal oxygen consumption is related to the transport of sodium. When the rate of sodium reabsorption was reduced during mannitol diuresis, arteriovenous oxygen difference decreased, but renal oxygen consumption remained unchanged. It is suggested that the active sodium transport in the proximal tubules continues at an unchanged rate during mannitol diuresis but that net reabsorption is reduced owing to increased passive influx into the tubular lumen when the transtubular concentration gradient increases. Other interpretations are discussed.

Effects of Chlormerodrin, p-Chloromercuribenzoate and Dichlorphenamide on Renal Sodium Reabsorption and Oxygen Consumption

Nephron ◽  
1964 ◽  
Vol 1 (4) ◽  
pp. 221-229 ◽  
Author(s):  
R.H. Kessler ◽  
S.W. Weinstein ◽  
F.D. Nash ◽  
M. Fujimoto
Keyword(s):  
Oxygen Consumption ◽  
Sodium Reabsorption ◽  
Renal Sodium Reabsorption
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