Purification and Characterization of Genetic Variants of Human Transferrins*

2015 ◽  
pp. 1178-1180
Author(s):  
F. W. Putnam ◽  
W. E. Roop
Keyword(s):  
Genetic Variants ◽  
Purification And Characterization

Researching the green fuse: an historical memoir

2002 ◽  
Vol 80 (12) ◽  
pp. 1231-1238
Author(s):  
John King
Keyword(s):  
Tissue Culture ◽  
Genetic Variants ◽  
Somatic Hybridization ◽  
Datura Innoxia ◽  
Purification And Characterization ◽  
Metabolic Intermediates ◽  
Physiology And Biochemistry ◽  
Biochemical Genetic ◽  
Branched Chain

The author outlines the major milestones in his 40-year career in plant research. His research program might be described, broadly, as explorations into the physiology and biochemistry of plants and their cells using physiological, biochemical, genetic, molecular biological and chemical techniques and technologies. The main areas encompassed by the program are the pathways of biosynthesis of branched-chain amino acids, pantothenate, folates and one-carbon units, uptake of organic and inorganic sources of nitrogen by plant cells, and somatic hybridization and genetic transformation of plant protoplasts. Many of these investigations were carried out with the aid of auxotrophic and resistance genetic variants selected either from cultures of Datura innoxia cells or from among mutagenized populations of Arabidopsis thaliana seedlings. Some technologies found to be especially useful were plant cell and tissue culture, detection of 14C-labelled isotopes in metabolic intermediates, isolation, purification and characterization of enzymes, and most recently, 13C nuclear magnetic resonance (NMR) spectroscopy.Key words: Arabidopsis, Datura, biochemical genetic variants, tissue culture, nitrogen metabolism.

Purification and characterization of phosphoglucomutase from peas

1994 ◽  
Vol 92 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Cynthia M. Galloway ◽  
W. Mack Dugger
Keyword(s):  
Purification And Characterization

Purification and Characterization of Tissue-Type Plasminogen Activator in Maxillary Mucosa with Chronic Inflammation

1985 ◽  
Vol 54 (02) ◽  
pp. 485-489 ◽  
Author(s):  
Yukiyoshi Hamaguchi ◽  
Masuichi Ohi ◽  
Yasuo Sakakura ◽  
Yasuro Miyoshi
Keyword(s):  
Plasminogen Activator ◽  
Chronic Inflammation ◽  
Gel Filtration ◽  
Potassium Acetate ◽  
Tissue Type ◽  
Purification And Characterization ◽  
Tissue Type Plasminogen Activator ◽  
Urokinase Inhibitor ◽  
Type Plasminogen Activator

SummaryTissue-type plasminogen activator (TPA) was purified from maxillary mucosa with chronic inflammation and compared with urokinase. Purification procedure consisted of the extraction from delipidated mucosa with 0.3M potassium acetate buffer (pH 4.2), 66% saturation of ammonium sulfate, zinc chelate-Sepharose, concanavalin A-Sepharose and Sephadex G-100 gel filtration chromatographies.The molecular weight of the TPA was approximately 58,000 ± 3,000. Its activity was enhanced in the presence of fibrin and was quenched by placental urokinase inhibitor, but not quenched by anti-urokinase antibody. The TPA made no precipitin line against anti-urokinase antibody, while urokinase did.All these findings indicate that the TPA in maxillary mucosa with chronic inflammation is immunologically dissimilar to urokinase and in its affinity for fibrin.

Prothrombin Metz : Purification and Characterization of a Variant of Human Prothrombin

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Factor Xa ◽  
Polyacrylamide Gel ◽  
Cleavage Pattern ◽  
Purification And Characterization ◽  
Double Immunodiffusion ◽  
The Family

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.

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