Freezing of Red Cells with Liquid Nitrogen: II. In vitro and in vivo Studies on the Viability of the Red Cells after Freezing and Thawing

2015 ◽  
pp. 687-691
Author(s):  
H. K. Prins ◽  
J. A. Loos ◽  
C. Z�rcher ◽  
A. E. G. Kr. von dem Borne
Keyword(s):  
Liquid Nitrogen ◽  
Red Cells ◽  
In Vivo Studies ◽  
Freezing And Thawing

IN VIVO STUDIES ON RED CELLS AND PLATELETS STORED IN HALFSTRENGTH CITRATE

1987 ◽  
Author(s):  
C Prewse ◽  
K Bell ◽  
B Griffin
Keyword(s):  
Factor Viii ◽  
Coagulation Factor ◽  
Red Cells ◽  
In Vivo Studies ◽  
Dramatic Improvement ◽  
Half Strength ◽  
Survival Studies ◽  
Cellular Components

We have previously shown that donation of blood into anticoagulants containing half the normal amount of citrate results in a dramatic improvement in the stability of coagulation factor VIII and has no adverse effect on the in vitro qualities of red cells or platelets during storage. To confirm the viability of stored cellular components we are now performing autologous survival studies in healthy volunteers using radiolabelled cells from red cells and platelets stored for 35 and 5 days respectively. Results to date indicate a 24 hour survival of 80% for red cells stored at a haematocrit of 0.70 for 35 days. Infusion of Ill-In oxine labelled platelets after storage for 5 days in full or half-strength citrate gave recoveries of 40% and survivals of 7 days. These encouraging results suggest use of halfstrength citrate may be a route to increasing factor VIII supply without any additional donor recruitment. Further in vitro studies have also been performed on cellular components and reveal adequate in vitro quality for half-strength citrate blood held at room temperature for 20 hours prior to component preparation.

Rapid freezing of the mouse blastocyst: effects of cryoprotectants and of time and temperature of exposure to cryoprotectant before direct plunging into liquid nitrogen

1991 ◽  
Vol 3 (2) ◽  
pp. 175 ◽  
Author(s):  
R Li ◽  
A Trounson
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Rapid Freezing ◽  
High Concentration ◽  
Freezing And Thawing ◽  
Initial Exposure ◽  
Survival And Development ◽  
High Concentrations

This study investigates the effects of time and temperature of exposure to a high concentration (4.5 M) of dimethyl sulfoxide (DMSO), glycerol, 1,2-propanediol (PROH), or a mixture of DMSO and glycerol (DG) in a solution containing 0.25 M sucrose, on the survival and development of rapidly frozen mouse blastocysts. Embryos had significantly (P less than 0.01) higher rates of survival and development when exposed to cryoprotectant at 0 degree C compared with room temperature. The time of exposure to cryoprotectant at either 0 degree C or room temperature before being plunged into liquid nitrogen significantly (P less than 0.01) affected the survival and development of frozen-thawed embryos. Survival and development of blastocysts in vitro and in vivo was significantly (P less than 0.05) higher when exposed at 0 degree C for 10 min to DG, DMSO and glycerol than to PROH. It is concluded that, unlike early-cleavage stage embryos, blastocysts need to be equilibrated at a low temperature (0 degree C) with high concentrations of cryoprotectant before rapid freezing. Exposure of blastocysts to 4.5 M cryoprotectant and 0.25 M sucrose at room temperature either was toxic or else markedly reduced their viability after freezing and thawing, depending on the duration of the initial exposure.

Relationship between Generation and Plasma Concentration of Anorganic Phosphorus. in Vivo Studies on Dialysis Patients and in Vitro Studies on Erythrocytes

1989 ◽  
Vol 12 (8) ◽  
pp. 524-532 ◽  
Author(s):  
H. Pogglitsch ◽  
W. Estelberger ◽  
W. Petek ◽  
S. Zitta ◽  
E. Ziak
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Plasma Concentration ◽  
Nonlinear Function ◽  
Extracellular Fluid ◽  
Red Cells ◽  
In Vitro Studies ◽  
In Vivo Studies

The plasma concentration of inorganic phosphorus (Pi) was determined before, during and after hemodialysis in 28 patients with chronic renal failure. Pi plasma concentration decreased rapidly when hemodialysis was started but did not fall below normal levels during continued dialysis. These changes of Pi concentration were fitted to a model of Pi kinetics in which Pi delivery to plasma is a nonlinear function of the extracellular Pi concentration. In separate in vitro studies, erythrocytes from six subjects with normal renal function and from 14 patients with chronic renal failure were incubated in homologous plasma with various amounts of Pi added. All other factors known to affect the Pi shift between intra - and extracellular fluid compartments (pH, calcium concentration) were kept constant. The relation between Pi concentration in plasma used for incubation and in red cells after 1h incubation suggested a mechanism in which a high plasma concentration results in movement of Pi into red cells where Pi is stored most probably in glucose esters. At low Pi plasma concentration Pi is delivered to plasma at a rate which cannot be explained solely by passive movement of intracellular Pi to plasma but requires additional generation from intracellular storage forms. The generation and delivery of Pi in patients and in their erythrocytes indicate a simple cell-mediated Pi homeostasis counter-acting abnormal fluctuations of plasma Pi.

Rejuvenation of Aged Human Red Cells Improves Their in Vivo Recovery in a Mouse Model

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 991-991
Author(s):  
Monique GelderMan-Fuhrmann ◽  
Jaroslav G. Vostal
Keyword(s):  
Mouse Model ◽  
Red Cells ◽  
In Vivo Studies ◽  
Red Cell ◽  
Fresh Rbcs ◽  
Gamma Irradiated ◽  
In Vivo Recovery ◽  
Human Rbcs

Evaluation of novel storage or processing technology for human red blood cells (RBCs) involves in vitro tests on the red cells to determine biochemical changes and in vivo studies in healthy human volunteers with radiolabeled red cells to determine in vivo recovery 24 hours post infusion. In vivo studies are needed because our understanding of red cell storage lesions is not sufficient to identify an in vitro test(s) that would adequately predict red cell performance in vivo. The clinical studies with radiolabeled cells are used as the gold standard for evaluation prior to approval of a novel technology by the FDA. However, in vivo studies require time and funds and can be a significant hurdle in the development of new products. An animal model that could predict performance of human red cells in vivo would be useful in the development process. We previously reported that severe combined immunodeficient (SCID) mice could be used as a model to identify damaged human platelets (Transfusion. 47(8):1540–9, 2007). In the current study, we investigated if this murine model could be used to distinguish between the recovery of fresh and aged human RBCs, non-rejuvenated and rejuvenated aged RBCs, gamma-irradiated (25 Gy) fresh RBCs and irradiated fresh RBCs and stored for 28 days. “Fresh” RBCs were processed from whole blood within 24 hrs of collection and the “aged” RBCs were either RBC products stored for 42 or 100 days in an additive solution at 4°C. For in vivo recovery, approximately 1x109 human RBCs were injected into the tail vein of SCID mice (n=5 or 7 per condition) and serial blood samples were collected. Human RBCs were detected in mouse whole blood by flow cytometry using an anti-human glycophorin A mAb (clone CLB-ery-1). Recovery was defined as percent of human RBCs in the mouse circulation at 2 hours post infusion. Rejuvenation of cells was accomplished by incubating RBCs for 1 hour with Rejuvesol solution (Table 1). 2,3-DPG Levels (mM/L) Pre- and Post-Rejuvenation Fresh RBCs Aged for 42 Days Aged for 100 Days Control 3.25±0.40 0.17±0.04 0.38 ±0.06 Rejuvenated 8.58±0.82 4.56±0.17 2.31±0.13 Fresh red cells exhibited recovery of 58.4±4.4 % of total cells injected. Aged RBCs showed a reduced in vivo recovery of 35.7±7.3 % and 5.7±1.6 % of total cells injected for 42 and 100 day old RBC, respectively. Gamma-irradiated fresh RBCs and irradiated fresh RBCs stored for 28 days showed a recovery of 66.7±8.6 % and 55±13.2 % respectively, whereas the recovery of control fresh RBCs and control fresh RBCs stored for 28 days showed a recovery of 58.4±4.4 % and 49.1±7.0 % (p=0.44) respectively (Table 2). In VivoRecovery Fresh RBCs Stored for 28 days Aged for 42 Days Aged for 100 Days nd - not determined Control 58.4±4.5 49.1±7.0 35.7±7.3 5.17±1.6 Rejuvenated 52.5±11.5 nd 55.4±7.1 21.3±5.0 Irradiated (25Gy) 66.7±8.6 55±13.2 nd nd Our data indicate that the SCID mouse model can distinguish between fresh and aged red cells and that rejuvenation of the red cells increases intracellular 2,3-DPG levels and in vivo recovery of aged red cells. The SCID mouse model could be used to develop or improve existing methods of red cell storage and processing. The findings and conclusions in this abstract have not been formally disseminated by the Food and Drug Administration and should not be construed to represent any Agency determination or policy.

In vitro and in vivo studies of new cationic Zn(II)‐benzonaphthoporphyrazines as photosensitizers for PDT

2001 ◽  
Vol 5 (8) ◽  
pp. 645-651
Author(s):  
M. Peeva ◽  
M. Shopova ◽  
U. Michelsen ◽  
D. Wöhrle ◽  
G. Petrov ◽  
...  
Keyword(s):  
In Vivo Studies

Effect of caffeine on theophyliine on cerebral blood flow response to brain activation: In vitro and in vivo studies

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S198-S198
Author(s):  
Joseph R Meno ◽  
Thien-son K Nguyen ◽  
Elise M Jensen ◽  
G Alexander West ◽  
Leonid Groysman ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Brain Activation ◽  
In Vivo Studies ◽  
Blood Flow Response ◽  
Flow Response

Effects of Unfractionated and Low Molecular Weight Heparins on Platelet Thromboxane Biosynthesis “In Vivo”

1994 ◽  
Vol 72 (06) ◽  
pp. 942-946 ◽  
Author(s):  
Raffaele Landolfi ◽  
Erica De Candia ◽  
Bianca Rocca ◽  
Giovanni Ciabattoni ◽  
Armando Antinori ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Unfractionated Heparin ◽  
Low Molecular Weight Heparin ◽  
Primary Source ◽  
In Vivo Studies ◽  
Low Molecular Weight ◽  
Heparin Administration ◽  
To Receive

SummarySeveral “in vitro” and “in vivo” studies indicate that heparin administration may affect platelet function. In this study we investigated the effects of prophylactic heparin on thromboxane (Tx)A2 biosynthesis “in vivo”, as assessed by the urinary excretion of major enzymatic metabolites 11-dehydro-TxB2 and 2,3-dinor-TxB2. Twenty-four patients who were candidates for cholecystectomy because of uncomplicated lithiasis were randomly assigned to receive placebo, unfractionated heparin, low molecular weight heparin or unfractionaed heparin plus 100 mg aspirin. Measurements of daily excretion of Tx metabolites were performed before and during the treatment. In the groups assigned to placebo and to low molecular weight heparin there was no statistically significant modification of Tx metabolite excretion while patients receiving unfractionated heparin had a significant increase of both metabolites (11-dehydro-TxB2: 3844 ± 1388 vs 2092 ±777, p <0.05; 2,3-dinor-TxB2: 2737 ± 808 vs 1535 ± 771 pg/mg creatinine, p <0.05). In patients randomized to receive low-dose aspirin plus unfractionated heparin the excretion of the two metabolites was largely suppressed thus suggesting that platelets are the primary source of enhanced thromboxane biosynthesis associated with heparin administration. These data indicate that unfractionated heparin causes platelet activation “in vivo” and suggest that the use of low molecular weight heparin may avoid this complication.

LACK OF EFFECT OF CORTICOSTEROIDS ON THE IN VIVO DISAPPEARANCE OF SULFHYDRYL-INHIBITED AND ANTIBODY-COATED ERYTHROCYTES

1964 ◽  
Vol 47 (3_Suppl) ◽  
pp. S28-S36
Author(s):  
Kailash N. Agarwal
Keyword(s):  
Red Cells ◽  
List Type ◽  
Red Cell

ABSTRACT Red cells were incubated in vitro with sulfhydryl inhibitors and Rhantibody with and without prior incubation with prednisolone-hemisuccinate. These erythrocytes were labelled with Cr51 and P32 and their disappearance in vivo after autotransfusion was measured. Prior incubation with prednisolone-hemisuccinate had no effect on the rate of red cell disappearance. The disappearance of the cells was shown to take place without appreciable intravascular destruction.

Effectiveness of the integration of quercetin, turmeric, and N-acetylcysteine in reducing inflammation and pain associated with endometriosis. In-vitro and in-vivo studies

2020 ◽  
Vol 72 (5) ◽  
Author(s):  
Mario Fadin ◽  
Maria C. Nicoletti ◽  
Marzia Pellizzato ◽  
Manuela Accardi ◽  
Maria G. Baietti ◽  
...  
Keyword(s):  
In Vivo Studies
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