Observations on Erythrocytic Reduced Glutathione Stability in Normal Subjects and in Certain Haematological Disorders

2015 ◽  
pp. 598-602
Author(s):  
J. B. Chatterjea ◽  
S. Swarup ◽  
S. K. Ghosh
Keyword(s):  
Reduced Glutathione ◽  
Normal Subjects ◽  
Haematological Disorders

scholarly journals Effect of glucose on reduced glutathione level in Malay uncomplicated type 2 diabetes patients

2021 ◽  
pp. 13
Author(s):  
Subandrate ◽  
Raafqi Ranasasmita
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Free Radical ◽  
Reduced Glutathione ◽  
Normal Subjects ◽  
Random Blood Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Patients

Background: Increasing blood sugar level may increase free radical compounds in type 2 diabetes. Free radical compounds can cause oxidative stress, thereby decreasing endogenous antioxidants such as reduced glutathione (GSH). Objective: This study aimed to determine whether random blood glucose levels affect GSH in type 2 diabetes patients within the Malay race. Methods: This study was observational with case-control, involving 25 patients with uncomplicated type 2 diabetes (receiving metformin and/or glimipiride) and 25 healthy controls. Random blood glucose levels were determined using ACCU-CHECK® Kit. Blood GSH levels were determined by Sigma GSH Assay Kit. Results: Results show that type 2 diabetes patients have a significantly lower random blood glucose level compared with those of age-matched normal subjects (p<0.0001). Type 2 diabetic patients had significantly lower levels of GSH (p=0.00) than those of age-matched normal subjects. We found a moderate negative correlation (r=-0.437 and p=0.02) between the level of random blood glucose and the level of GSH. Conclusion: The depletion of GSH during hyperglycemia may neutralize the free radicals indirectly generated by the abundant of glucose.  

Red Cell Glutathione in Anaemia

1973 ◽  
Vol 18 (6) ◽  
pp. 177-181 ◽  
Author(s):  
J. Hopkins ◽  
G. R. Tudhope
Keyword(s):  
Inverse Relationship ◽  
Reduced Glutathione ◽  
Haemoglobin Concentration ◽  
Normal Subjects ◽  
Initial Therapy ◽  
Megaloblastic Anaemia ◽  
Red Cells ◽  
Red Cell ◽  
A Value ◽  
Normal Mean

Erythrocyte reduced glutathione (GSH) has been measured in 17 normal subjects and in 70 patients with anaemia of various causes; the red cells of most of the anaemic patients contained abnormally high levels of GSH. In the group of cases of untreated megaloblastic anaemia in which the haemoglobin concentration was less than 8.0 g. per 100 ml., there was an inverse relationship between the erythrocyte GSH level and the haemoglobin concentration. During initial therapy in megaloblastic anaemia, as the haemoglobin concentration increased, the GSH level decreased towards normal; the time taken for the GSH level to reach a value 2 standard deviations above the normal mean was inversely related to the initial haemoglobin concentration. In the red cells of patients with anaemia there was no correlation between the GSH level and the activity of glutathione peroxidase. The significance of these findings is discussed.

Systemic deficiency of glutathione in cystic fibrosis

1993 ◽  
Vol 75 (6) ◽  
pp. 2419-2424 ◽  
Author(s):  
J. H. Roum ◽  
R. Buhl ◽  
N. G. McElvaney ◽  
Z. Borok ◽  
R. G. Crystal
Keyword(s):  
Cystic Fibrosis ◽  
Reduced Glutathione ◽  
Chronic Obstructive Lung Disease ◽  
Inflammatory Cells ◽  
Normal Subjects ◽  
Chronic Obstructive ◽  
Lung Pathology ◽  
Epithelial Surface ◽  
Glutathione Deficiency ◽  
Respiratory Epithelial

Cystic fibrosis (CF), a disorder characterized by mutations of the CF transmembrane regulator gene, is characterized in the lung by chronic inflammation, leading to progressive damage to the airway epithelium, bronchiectasis, and chronic obstructive lung disease. One process contributing to the airway derangement is the chronic burden of oxidants released by inflammatory cells on the respiratory epithelial surface. With this background, we hypothesized that glutathione in respiratory epithelial lining fluid (ELF) in CF patients might be oxidized and/or diminished in amount compared with that in normal subjects. Recovery of ELF by bronchoalveolar lavage from young adults with CF (n = 21) and normal subjects (n = 25) demonstrated marked neutrophil-dominated inflammation in ELF in CF patients. As predicted, ELF in CF patients was characterized by a deficiency of glutathione (P < 0.001), but this was secondary to a reduction in reduced glutathione (P < 0.001), inasmuch as there were no differences in ELF levels of oxidized glutathione (P > 0.2). Unexpectedly, there was also a marked deficiency of reduced glutathione in plasma (P < 0.02); i.e., the glutathione "deficiency" observed in ELF in CF patients is not limited to the site of the inflammation but is systemic. Although the etiology of this generalized deficiency of extracellular glutathione is unknown, it is important in considering options for treating the concomitant and devastating lung pathology in this disorder.

Serum concentration of cystatin C is not affected by cellular proliferation in patients with proliferative haematological disorders

2002 ◽  
Vol 39 (3) ◽  
pp. 308-310 ◽  
Author(s):  
OA Mojiminiyi ◽  
R Marouf ◽  
N Abdella ◽  
M Kortom ◽  
R Abdul-Razzak
Keyword(s):  
Cystatin C ◽  
Blood Count ◽  
Cellular Proliferation ◽  
Normal Subjects ◽  
Full Blood Count ◽  
Serum Cystatin C ◽  
Serum Cystatin ◽  
Gault Formula ◽  
Haematological Disorders ◽  
Apparently Healthy

Background Recent reports have shown elevated concentrations of serum cystatin C (CC) in patients with solid tumours. The aim of our study was to investigate whether patients with proliferative haematological disorders have increased serum concentrations of CC compared with normal subjects. Methods Full blood count, serum CC, β2-microglobulin (β2M) and serum creatinine were determined in 27 apparently healthy subjects and 35 patients with proliferative haematological disorders. Creatinine clearance was calculated using the Cockroft-Gault formula. Results CC and β2M showed a significant correlation with each other ( r = 0·96, P < 0·0001). Serum β2M ( P = 0·001) and the serum β2M:CC ratio ( P = 0·001) were significantly higher in the patients with normal renal function than in controls, but serum CC was not significantly different ( P = 0·08). CC did not show a significant correlation with blood count parameters. Discussion Compared with β2M and the β2M:CC ratio, serum CC is not influenced by cell proliferation in haematological disorders.

Auditory Selective Attention in Cerebral-Palsied Individuals

1985 ◽  
Vol 16 (4) ◽  
pp. 260-266 ◽  
Author(s):  
Lee Ann Laraway
Keyword(s):  
Selective Attention ◽  
White Noise ◽  
Normal Subjects ◽  
Auditory Selective Attention ◽  
Normal Individuals ◽  
Significant Difference

The purpose of this study was to determine whether there is a statistically significant difference between the auditory selective attention abilities of normal and cerebral-palsied individuals. Twenty-three cerebral-palsied and 23 normal subjects between the ages of 5 and 21 were asked to repeat a series of 30 items consisting of from 2 to 4 digits in the presence of intermittent white noise. Results of the study indicate that cerebral-palsied individuals perform significantly poorer than normal individuals when the stimulus is accompanied by noise. Noise was not a significant factor in the performance of the normal subjects regardless of age.

The Effect of Stapedectomy on the Loudness of One’s Own Voice

1965 ◽  
Vol 8 (3) ◽  
pp. 223-234 ◽  
Author(s):  
William Melnick
Keyword(s):  
Middle Ear ◽  
Normal Subjects ◽  
Sound Energy ◽  
Loudness Function ◽  
Before And After ◽  
Increased Sensitivity

Five subjects with normal middle ear mechanisms, and otosclerotic patients, before and after stapedectomy, matched the loudness of their voices to the loudness of a 125-cps-sawtooth noise. The results showed loudness matching functions with gradual slopes, less than 1.00, for the normal subjects and the patients prior to stapedectomy. Post-surgically, the loudness function for the patients increased in steepness to considerably more than 1.00. These results are explained, most logically, in terms of increased sensitivity of the altered middle ear to sound energy generated by the listener’s own voice.

Delayed Auditory Feedback with Different Delay Times at Each Ear

1964 ◽  
Vol 7 (4) ◽  
pp. 369-371
Author(s):  
Samuel Fillenbaum
Keyword(s):  
Delay Interval ◽  
Auditory Feedback ◽  
Normal Subjects ◽  
Delayed Auditory Feedback ◽  
Delay Times

Binaurally asynchronous delayed auditory feedback (DAF) was compared with synchronous DAF in 80 normal subjects. Asynchronous DAF (0.10 sec difference) did not yield results different from those obtained under synchronous DAF with a 0.20 sec delay interval, an interval characteristically resulting in maximum disruptions in speech.

Object Transformations in the Play of Language-Impaired Children

1988 ◽  
Vol 53 (4) ◽  
pp. 459-466 ◽  
Author(s):  
Brenda Y. Terrell ◽  
Richard G. Schwartz
Keyword(s):  
Normal Subjects ◽  
Play Behavior ◽  
Chronological Age ◽  
Standard Group ◽  
Language Impaired ◽  
Normal Language ◽  
Mean Length Of Utterance ◽  
Impaired Children

The play behavior of 10 language-impaired children was observed. Their performances in play were compared to those of 10 normal-language children matched for chronological age as well as to those of 10 normal-language children matched for mean length of utterance. The children were observed as they played spontaneously with a standard group of toys and as they played with objects that required object transformations for successful play. The chronological age-matched normal subjects showed a trend toward performance of more object transformations in play than either the language-impaired or younger normal-language children. Additionally, although object transformations were observed in both segments, all children performed more object transformations with objects than with toys.

Sign in / Sign up

Export Citation Format

Share Document