scholarly journals Comparison of Histochemical Staining Methods and Correlation with Transient Elastography in Acute Hepatitis

Pathobiology ◽  
2015 ◽  
Vol 82 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Daniela Cabibi ◽  
Vincenza Calvaruso ◽  
Letizia Giuffrida ◽  
Sabrina Ingrao ◽  
Laura Balsamo ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Transient Elastography ◽  
Histochemical Staining ◽  
Staining Methods

scholarly journals Role of Transient Elastography (Fibroscan) in Differentiating Severe Acute Hepatitis and Acute on Chronic Liver Failure

2015 ◽  
Vol 5 (4) ◽  
pp. 303-309 ◽  
Author(s):  
Praveen Sharma ◽  
Rinkesh Bansal ◽  
Abdul Matin ◽  
Pankaj Tyagi ◽  
Naresh Bansal ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Hepatitis ◽  
Transient Elastography ◽  
Chronic Liver ◽  
Chronic Liver Failure ◽  
Severe Acute Hepatitis

scholarly journals Amyloid in Canine Mammary Tumors

1985 ◽  
Vol 22 (4) ◽  
pp. 347-354 ◽  
Author(s):  
J. H. Vos ◽  
E. Gruys
Keyword(s):  
Epithelial Cells ◽  
Mammary Tumors ◽  
Histochemical Staining ◽  
Amyloid Deposition ◽  
Corpora Amylacea ◽  
Mammary Carcinomas ◽  
Canine Mammary Tumors ◽  
Stromal Tissue ◽  
Staining Methods ◽  
Neoplastic Epithelial Cells

In canine mammary carcinomas, amyloid was present as amyloid-containing corpora amylacea and as local deposits between neoplastic epithelial cells or in stromal tissue. Histochemical staining methods revealed that this amyloid was not of the AA-type amyloid and contained tryptophan. The possible pathogenesis of this amyloid deposition is discussed.

Uneven distribution of preservative in kiln-dried sapwood lumber of Scots pine: Impact of wood structure and resin allocation

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Sheikh Ali Ahmed ◽  
Margot Sehlstedt-Persson ◽  
Olov Karlsson ◽  
Tom Morén
Keyword(s):  
Scots Pine ◽  
Wood Preservative ◽  
Wood Properties ◽  
Histochemical Staining ◽  
Kiln Drying ◽  
Specific Distribution ◽  
Ray Cells ◽  
Staining Methods ◽  
Staining Techniques ◽  
Resin Canals

Abstract Scots pine (Pinus sylvestris L.) sapwood lumber was collected after kiln drying and preservative treatment with Celcure AC 800 (a copper-amine wood preservative). Distribution of the preservative throughout the lumber was visually examined. Not all, but some samples showed specific localized areas without any preservative distribution throughout their entire length. Those samples were assessed further for anatomical properties, specifically in impregnated and unimpregnated areas. Additional study was conducted on the morphological nature and redistribution of lipophilic extractives using three different histochemical staining methods. Intrinsic wood properties – especially the frequency of axial resin canals and the percentage of canals blocked – were found to be responsible for the irregular distribution of the preservative. Furthermore, the inability to create continuous and frequent interstitial spaces due to the collapse of thin-walled ray cells throughout the lumber resulted in un-even distribution of preservatives. Staining techniques were useful to localize places with more or less abundance of extractives (e.g., fats) in impregnated and unimpregnated wood, which varied considerably. Histochemical observations revealed information pertaining to the kiln dry specific distribution and redistribution of extractives between the areas. Moreover, resin reallocation and modification in ray parenchyma and resin canals induced by kiln drying would be another reason for the impregnation anomalies.

scholarly journals The islet ghrelin cell

2013 ◽  
Vol 52 (1) ◽  
pp. R35-R49 ◽  
Author(s):  
Nils Wierup ◽  
Frank Sundler ◽  
R Scott Heller
Keyword(s):  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Developmental Regulation ◽  
Genetic Regulation ◽  
Cell Types ◽  
Human Islets ◽  
Histochemical Staining ◽  
Pancreatic Tumours ◽  
Age Related ◽  
Staining Methods

The islets of Langerhans are key regulators of glucose homeostasis and have been known as a structure for almost one and a half centuries. During the twentieth century several different cell types were described in the islets of different species and at different developmental stages. Six cell types with identified hormonal product have been described so far by the use of histochemical staining methods, transmission electron microscopy, and immunohistochemistry. Thus, glucagon-producing α-cells, insulin-producing β-cells, somatostatin-producing δ-cells, pancreatic polypeptide-producing PP-cells, serotonin-producing enterochromaffin-cells, and gastrin-producing G-cells have all been found in the mammalian pancreas at least at some developmental stage. Species differences are at hand and age-related differences are also to be considered. Eleven years ago a novel cell type, the ghrelin cell, was discovered in the human islets. Subsequent studies have shown the presence of islet ghrelin cells in several animals, including mouse, rat, gerbils, and fish. The developmental regulation of ghrelin cells in the islets of mice has gained a lot of interest and several studies have added important pieces to the puzzle of molecular mechanisms and the genetic regulation that lead to differentiation into mature ghrelin cells. A body of evidence has shown that ghrelin is an insulinostatic hormone, and the potential for blockade of ghrelin signalling as a therapeutic avenue for type 2 diabetes is intriguing. Furthermore, ghrelin-expressing pancreatic tumours have been reported and ghrelin needs to be taken into account when diagnosing pancreatic tumours. In this review article, we summarise the knowledge about islet ghrelin cells obtained so far.

Transient elastography assessment of the liver stiffness dynamics during acute hepatitis B

2010 ◽  
Vol 22 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Mauro Viganò ◽  
Sara Massironi ◽  
Pietro Lampertico ◽  
Massimo Iavarone ◽  
Silvia Paggi ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Acute Hepatitis ◽  
Transient Elastography ◽  
Liver Stiffness ◽  
Acute Hepatitis B

scholarly journals A QUANTITATIVE HISTOCHEMICAL STUDY OF INTESTINAL MUCOSA AFTER X-IRRADIATION

1970 ◽  
Vol 18 (4) ◽  
pp. 291-301 ◽  
Author(s):  
H. GALJAARD ◽  
J. BUYS ◽  
M. VAN DUUREN ◽  
J. GIESEN
Keyword(s):  
Esterase Activity ◽  
Histochemical Staining ◽  
Nonspecific Esterase ◽  
Radiation Doses ◽  
Functional Consequences ◽  
X Irradiation ◽  
Staining Methods ◽  
Quantitative Analyses ◽  
Nonspecific Esterase Activity ◽  
Crypt Cells

The effect of low doses of x-irradiation (50-400 R) on the activity of various enzymes in rat intestinal epithelium has been investigated by histochemical staining methods and quantitative microchemical analyses of crypts and villi dissected from frozen-dried sections. Irradiation had no effect on the activities of enzymes which in nonirradiated animals are present exclusively or mainly in the villus epithelium (aminopeptidase, various phosphatases) or are evenly distributed along the epithelium of crypts and villi (various dehydrogenases). However, nonspecific esterase activity decreased markedly both in crypt epithelium and villus epithelium. This occurred 36 hr after irradiation, independent of the radiation dose. The number of crypt cells with reduced esterase activity and the duration of the effect increased with higher radiation doses. These results were confirmed by quantitative analyses which also showed that esterase activity is 5 times higher in the villus than in the crypt. The remarkable correspondance between the period of reduced esterase activity in the crypt and the period of increased proliferative activity after various radiation doses suggest a relationship between changes in crypt cell population dynamics and esterase activity; the functional consequences for the villus epithelium of changes in the crypt cells after irradiation are discussed.

scholarly journals Transient elastography in acute hepatitis: All that's stiff is not fibrosis

Hepatology ◽  
2008 ◽  
Vol 47 (2) ◽  
pp. 370-372 ◽  
Author(s):  
Jeremy F L Cobbold ◽  
Simon D Taylor-Robinson
Keyword(s):  
Acute Hepatitis ◽  
Transient Elastography

SOME PROPERTIES OF THE SOLUBLE ESTERASES OF HUMAN LIVER

1961 ◽  
Vol 39 (9) ◽  
pp. 1329-1332 ◽  
Author(s):  
D. J. Ecobichon ◽  
W. Kalow
Keyword(s):  
Human Serum ◽  
Human Liver ◽  
Histochemical Staining ◽  
Enzymatic Properties ◽  
Water Soluble ◽  
Electrophoretic Migration ◽  
Zone Electrophoresis ◽  
Starch Gel ◽  
Staining Methods

Zone electrophoresis on starch gel in conjunction with various histochemical staining methods was applied to the study of the water-soluble esterases of liver. The results indicated that in regard to electrophoretic migration and enzymatic properties, none of the human liver esterases was identical with any of the human serum esterases.

Role of Transient Elastography (Fibroscan) in Differentiating Severe Acute Hepatitis and Acute on Chronic Liver Failure

2015 ◽  
Vol 13 (7) ◽  
pp. e114
Author(s):  
Rinkesh Kumar Bansal ◽  
Praveen Sharma ◽  
Pankaj Tyagi ◽  
Ashish Kumar ◽  
Vikas Singla ◽  
...  
Keyword(s):  
Liver Failure ◽  
Acute Hepatitis ◽  
Transient Elastography ◽  
Chronic Liver ◽  
Chronic Liver Failure ◽  
Severe Acute Hepatitis
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