An Immunodiagnostic Assay for Quantitation of Specific IgE to the Major Pollen Allergen Component, Pas n 1, of the Subtropical Bahia Grass

2014 ◽  
Vol 165 (4) ◽  
pp. 219-228 ◽  
Author(s):  
Victoria L. Timbrell ◽  
Lindsay Riebelt ◽  
Claire Simmonds ◽  
Graham Solley ◽  
William B. Smith ◽  
...  
Keyword(s):  
Specific Ige ◽  
Pollen Allergen ◽  
Allergen Component ◽  
Bahia Grass

scholarly journals Comparative study of the complement-activating and specific IgE-binding properties of ragweed pollen allergen

1997 ◽  
Vol 108 (1) ◽  
pp. 122-127 ◽  
Author(s):  
T. HIDVÉGI ◽  
L. BERRENS ◽  
L. VARGA ◽  
F. MARAÑON ◽  
B. SCHMIDT ◽  
...  
Keyword(s):  
Comparative Study ◽  
Specific Ige ◽  
Pollen Allergen ◽  
Ragweed Pollen ◽  
Binding Properties ◽  
Ige Binding

scholarly journals Specific IgE response to different grass pollen allergen components in children undergoing sublingual immunotherapy

2012 ◽  
Vol 10 (1) ◽  
Author(s):  
Francesco Marcucci ◽  
Laura Sensi ◽  
Cristoforo Incorvaia ◽  
Ilaria Dell’Albani ◽  
Giuseppe Di Cara ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Sublingual Immunotherapy ◽  
Specific Ige ◽  
Pollen Allergen ◽  
Allergen Components ◽  
Grass Pollen Allergen ◽  
Ige Response

Bahia grass pollen specific IgE is common in seasonal rhinitis patients but has limited cross-reactivity with Ryegrass

Allergy ◽  
2005 ◽  
Vol 60 (2) ◽  
pp. 251-255 ◽  
Author(s):  
J. M. Davies ◽  
M. L. Bright ◽  
J. M. Rolland ◽  
R. E. O'Hehir
Keyword(s):  
Grass Pollen ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Bahia Grass

Unique and Cross-Reactive T Cell Epitope Peptides of the Major Bahia Grass Pollen Allergen, Pas n 1

2012 ◽  
Vol 159 (4) ◽  
pp. 355-366 ◽  
Author(s):  
Tamara Etto ◽  
Carmela de Boer ◽  
Sara Prickett ◽  
Leanne M. Gardner ◽  
Astrid Voskamp ◽  
...  
Keyword(s):  
T Cell ◽  
Grass Pollen ◽  
Cell Epitope ◽  
Pollen Allergen ◽  
T Cell Epitope ◽  
Grass Pollen Allergen ◽  
Bahia Grass

scholarly journals Pollen allergen skin test and specific IgE reactivity among Filipinos: a community-based study

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Maureen Sabit ◽  
Cecil Wong ◽  
Agnes Andaya ◽  
John Donnie Ramos
Keyword(s):  
Skin Test ◽  
Specific Ige ◽  
Pollen Allergen ◽  
Community Based ◽  
Ige Reactivity

scholarly journals Luciferase Immunoprecipitation Systems immunoassay is a sensitive, rapid method to detect allergen component-specific IgE

2020 ◽  
Author(s):  
Adora A. Lin ◽  
Natalia S. Perez ◽  
Pamela A. Frischmeyer-Guerrerio ◽  
Thomas B. Nutman
Keyword(s):  
Dynamic Range ◽  
Specific Ige ◽  
Turnaround Time ◽  
Sample Volume ◽  
Small Sample ◽  
Flexible Tool ◽  
Allergen Component ◽  
Fel D 1 ◽  
Broad Dynamic Range ◽  
Allergen Extracts

AbstractCurrent assays to detect allergen-specific IgE have constraints related to obtaining pure, conformationally active allergen, variability in allergen extracts, sample volume required, and turnaround time. The luciferase immunoprecipitation systems (LIPS) immunoassay is a fast, sensitive assay created using recombinant antigens that requires a low specimen volume. These assays can also be easily modified to detect multiple antigens and antibody isotypes. Here, we demonstrate the use of LIPS assays as an innovative method to quantitatively measure allergen component-specific IgE in small sample volumes. Sera from healthy volunteers, helminth-infected adults, and peanut-allergic children were screened for IgE to cat using ImmunoCAP. These samples were also measured for IgE against Fel d 1 using LIPS. LIPS signal correlated to cat IgE levels with rS = 0.6204, p < 0.001. The LIPS signal: noise ratio differed significantly between cat IgE-samples and cat IgE+ samples with values > 0.5 kU/L, with the ability to differentiate cat IgE – individuals from cat IgE+ individuals with 85% sensitivity and 76% specificity. Given their rapidity, efficiency, sensitivity, and quantitation over a broad dynamic range, LIPS immunoassays can be a robust and flexible tool with potential uses in allergy research, diagnostics, and treatment.

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