Stimulation of Cyclooxygenase 2 Expression in Rat Peritoneal Mesothelial Cells

2014 ◽  
Vol 128 (3-4) ◽  
pp. 89-97 ◽  
Author(s):  
Michael E. Ullian ◽  
Louis M. Luttrell ◽  
Mi-Hye Lee ◽  
Thomas A. Morinelli
Keyword(s):  
Mesothelial Cells ◽  
Cyclooxygenase 2 ◽  
Peritoneal Mesothelial Cells ◽  
Stimulation Of

Changes in Biocompatibility of Dialysis Fluid during Its Dwell in the Peritoneal Cavity

1995 ◽  
Vol 15 (2) ◽  
pp. 152-157 ◽  
Author(s):  
Andrzej Breborowicz ◽  
Leo Martis ◽  
Dimitrios G. Oreopoulos
Keyword(s):  
Peritoneal Dialysis ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Mesothelial Cells ◽  
Mitogenic Effect ◽  
Dialysis Fluid ◽  
Peritoneal Mesothelial Cells ◽  
Noncollagen Proteins ◽  
Dialysis Solutions ◽  
Stimulation Of

Objective To evaluate the changes in biocompatibility of peritoneal dialysis solutions during intraperitoneal dwell. Design We studied the effect of the drained dialysates at time 0 and after 30, 60, 120, 240, and 360 minutes of intraperitoneal dwell on the growth of peritoneal mesothelial cells and fibroblasts and the synthesis of proteins by these cells. On one day the patients were dialyzed with glucose-based Dianeal and on alternate days with an amino acid-containing solution based on Travasol. Patients Dialysates were collected from 4 patients during continuous ambulatory peritoneal dialysis (CAPD) training. Results Unused dialysis solutions containing glucose or amino acids inhibit growth of mesothelial cells and fibroblasts. Dialysates obtained after 30 or 60 minutes of intraperitoneal dwell support the growth of these cells in a way similar to 10% fetal calf serum, but dialysates drained after a longer dwell of 120 360 minutes had a stronger effect on growth of these cells than did serum. All glucose-based dialysates stimulate the synthesis of collagen in mesothelial cells, whereas they reduce the synthesis of non-collagen proteins. All glucose-based dialysates reduce the synthesis of collagen and noncollagen proteins in fibroblasts compared with the production of these proteins in the presence of serum. Conclusion Changes in the properties of the dialysis solutions during their intraperitoneal dwells do not seem to increase their biocompatibility. Indeed, excessive mitogenic effect and the stimulation of collagen synthesis of the dialysates may induce pathological changes in the peritoneum.

scholarly journals Senescent Peritoneal Mesothelial Cells Promote Ovarian Cancer Cell Adhesion

2009 ◽  
Vol 174 (4) ◽  
pp. 1230-1240 ◽  
Author(s):  
Krzysztof Ksiazek ◽  
Justyna Mikula-Pietrasik ◽  
Katarzyna Korybalska ◽  
Grzegorz Dworacki ◽  
Achim Jörres ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Adhesion ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Cancer Cell Adhesion

scholarly journals High glucose levels inhibit focal adhesion kinase-mediated wound healing of rat peritoneal mesothelial cells

2003 ◽  
Vol 63 (2) ◽  
pp. 722-731 ◽  
Author(s):  
Masahito Tamura ◽  
Akihiko Osajima ◽  
Shingo Nakayamada ◽  
Hirofumi Anai ◽  
Narutoshi Kabashima ◽  
...  
Keyword(s):  
Wound Healing ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
High Glucose ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Glucose Levels

Mesothelial Cells

2007 ◽  
Vol 27 (2_suppl) ◽  
pp. 110-115 ◽  
Author(s):  
Susan Yung ◽  
Chan Tak Mao
Keyword(s):  
Mesothelial Cell ◽  
In Vitro Models ◽  
Mesothelial Cells ◽  
Dynamic Membrane ◽  
Peritoneal Mesothelial Cells ◽  
Immunohistochemical Markers ◽  
Vitro Model ◽  
And Function

♦ Background The introduction of peritoneal dialysis (PD) as a modality of renal replacement therapy has provoked much interest in the biology of the peritoneal mesothelial cell. Mesothelial cells isolated from omental tissue have immunohistochemical markers that are identical to those of mesothelial stem cells, and omental mesothelial cells can be cultivated in vitro to study changes to their biologic functions in the setting of PD. ♦ Method The present article describes the structure and function of mesothelial cells in the normal peritoneum and details the morphologic changes that occur after the introduction of PD. Furthermore, this article reviews the literature of mesothelial cell culture and the limitations of in vitro studies. ♦ Results The mesothelium is now considered to be a dynamic membrane that plays a pivotal role in the homeostasis of the peritoneal cavity, contributing to the control of fluid and solute transport, inflammation, and wound healing. These functional properties of the mesothelium are compromised in the setting of PD. Cultures of peritoneal mesothelial cells from omental tissue provide a relevant in vitro model that allows researchers to assess specific molecular pathways of disease in a distinct population of cells. Structural and functional attributes of mesothelial cells are discussed in relation to long-term culture, proliferation potential, age of tissue donor, use of human or animal in vitro models, and how the foregoing factors may influence in vitro data. ♦ Conclusions The ability to propagate mesothelial cells in culture has resulted, over the past two decades, in an explosion of mesothelial cell research pertaining to PD and peritoneal disorders. Independent researchers have highlighted the potential use of mesothelial cells as targets for gene therapy or transplantation in the search to provide therapeutic strategies for the preservation of the mesothelium during chemical or bacterial injury.

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