Genome-Wide Profiling of DNA Methylation and Tumor Progression in Human Hepatocellular Carcinoma

2014 ◽  
Vol 32 (6) ◽  
pp. 658-663 ◽  
Author(s):  
Naoshi Nishida ◽  
Takafumi Nishimura ◽  
Takuya Nakai ◽  
Hirokazu Chishina ◽  
Tadaaki Arizumi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Tumor Progression ◽  
Human Hepatocellular Carcinoma ◽  
Genome Wide

Reduction of T-Box 15 gene expression in tumor tissue as a prognostic biomarker for patients with hepatocellular carcinoma.

2018 ◽  
Vol 36 (4_suppl) ◽  
pp. 341-341
Author(s):  
Yuji Morine ◽  
Tohru Utsunomiya ◽  
Satoru Imura ◽  
Tetsuya Ikemoto ◽  
Shuichi Iwahashi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Tumor Progression ◽  
Tumor Tissue ◽  
Mrna Levels ◽  
T Box ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Tumor Tissues ◽  
A Genome

341 Background: Genome-wide analysis is widely applied to detect molecular alterations in tumor and non-tumor tissue during oncogenesis and tumor progression. We analyzed DNA methylation profiles of hepatocellular carcinoma (HCC), which was is the third-highest cause of cancer-related deaths worldwide, and investigated the clinical role of most heyper-methylated gene, encodes T-box 15 (TBX15), which was originally involved in mesodermal differentiation. Methods: We used an Illumina Infinium HumanMethylation450 BeadChip Kit to conduct a genome-wide analysis of DNA methylation of tumor and non-tumor tissue of 15 patients with HCC. Methylation scores for CpG sites were assigned a Beta-value. Another validation set, which comprised 58 patients with HCC who underwent radical resection, was analyzed to investigate the relationships between tumor phenotype, prognosis, and TBX15 mRNA expression. Results: TBX15 was the most hyper-methylated gene (Beta-value in tumor tissue = 0.52 compared with non-tumor tissue), and TBX15 mRNA levels in tumor tissues were significantly lower compared with those of non-tumor tissues (p < 0.0001). When we assigned a cutoff value = 0.5-fold to define differential expression of TBX15 mRNA, low TBX15 expression significantly correlated with higher serum DCP levels, and the overall survival 5-year survival rates of the low-expression group (n = 17) were significantly shorter compared with those of the high-expression group (n = 41) (43.3% vs. 86.2%, p = 0.001). Multivariate analysis identified low TBX15 expression as an independent prognostic factor for overall and disease-free survival. Conclusions: Genome-wide DNA methylation profiling indicates that hypermethylation and reduced expression of TBX15 in tumor tissue represents a potential biomarker for predicting tumor progression and poor survival of patients with HCC.

Abstract 4996: Genome-wide DNA methylation profiles in hepatocellular carcinoma

2012 ◽  
Author(s):  
Jing Shen ◽  
Shuang Wang ◽  
Yujing Zhang ◽  
Maya Kappil ◽  
Hui-Chen Wu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Genome Wide

Genome-wide profiling of DNA methylation reveals preferred sequences of DNMTs in hepatocellular carcinoma cells

Tumor Biology ◽  
2015 ◽  
Vol 37 (1) ◽  
pp. 877-885 ◽  
Author(s):  
Hong Fan ◽  
Zhujiang Zhao ◽  
Yuchao Cheng ◽  
He Cui ◽  
Fengchang Qiao ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Carcinoma Cells ◽  
Hepatocellular Carcinoma Cells ◽  
Genome Wide

scholarly journals Exploring genome-wide DNA methylation profiles altered in hepatocellular carcinoma using Infinium HumanMethylation 450 BeadChips

Epigenetics ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. 34-43 ◽  
Author(s):  
Jing Shen ◽  
Shuang Wang ◽  
Yu-Jing Zhang ◽  
Hui-Chen Wu ◽  
Muhammad G. Kibriya ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Genome Wide
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