A Novel Wheat-Dasypyrum breviaristatum Substitution Line with Stripe Rust Resistance

2014 ◽  
Vol 143 (4) ◽  
pp. 280-287 ◽  
Author(s):  
Guang-Rong Li ◽  
Jin-Mei Zhao ◽  
Dong-Hai Li ◽  
En-Nian Yang ◽  
Yu-Feng Huang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Dasypyrum Breviaristatum

scholarly journals Characterization of a wheat–tetraploid Thinopyrum elongatum 1E(1D) substitution line K17–841-1 by cytological and phenotypic analysis and developed molecular markers

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Daiyan Li ◽  
Juwei Zhang ◽  
Haijiao Liu ◽  
Binwen Tan ◽  
Wei Zhu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Head Blight ◽  
Phenotypic Analysis ◽  
Complex Species ◽  
Thinopyrum Elongatum ◽  
Promising Source

Abstract Background Tetraploid Thinopyrum elongatum (2n = 4x = 28) is a promising source of useful genes, including those related to adaptability and resistance to diverse biotic (Fusarium head blight, rust, powdery mildew, and yellow dwarf virus) and abiotic (cold, drought, and salt) stresses. However, gene transfer rates are low for this species and relatively few species-specific molecular markers are available. Results The wheat-tetraploid Th. elongatum line K17–841-1 derived from a cross between a hexaploid Trititrigia and Sichuan wheat cultivars was characterized based on sequential genomic and fluorescence in situ hybridizations and simple sequence repeat markers. We revealed that K17–841-1 is a 1E (1D) chromosomal substitution line that is highly resistant to stripe rust pathogen strains prevalent in China. By comparing the sequences generated during genotyping-by-sequencing (GBS), we obtained 597 specific fragments on the 1E chromosome of tetraploid Th. elongatum. A total of 235 primers were designed and 165 new Th. elongatum-specific markers were developed, with an efficiency of up to 70%. Marker validation analyses indicated that 25 specific markers can discriminate between the tetraploid Th. elongatum chromosomes and the chromosomes of other wheat-related species. An evaluation of the utility of these markers in a F2 breeding population suggested these markers are linked to the stripe rust resistance gene on chromosome 1E. Furthermore, 28 markers are unique to diploid Th. elongatum, tetraploid Th. elongatum, or decaploid Thinopyrum ponticum, which carry the E genome. Finally, 48 and 74 markers revealed polymorphisms between Thinopyrum E-genome- containing species and Thinopyrum bessarabicum (Eb) and Pseudoroegneria libanotica (St), respectively. Conclusions This new substitution line provide appropriate bridge–breeding–materials for alien gene introgression to improve wheat stripe rust resistance. The markers developed using GBS technology in this study may be useful for the high-throughput and accurate detection of tetraploid Th. elongatum DNA in diverse materials. They may also be relevant for investigating the genetic differences and phylogenetic relationships among E, Eb, St, and other closely-related genomes and for further characterizing these complex species.

Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines with Superior Gene(s) for Spike Length and Stripe Rust Resistance

2018 ◽  
Vol 156 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Hongjin Wang ◽  
Zhihui Yu ◽  
Bin Li ◽  
Tao Lang ◽  
Guangrong Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Field Evaluation ◽  
Spike Length ◽  
Stripe Rust Resistance ◽  
Stripe Rust Resistance Gene ◽  
Fraction Length ◽  
Dasypyrum Breviaristatum

Dasypyrum breviaristatum (genome VbVb) contains potentially important traits for commercial wheat production. Chromosome 2Vb of D. breviaristatum carries several desirable agronomic characters, including long spike length as well as enhanced resistance to stripe rust, which are expressed in a common wheat background. In this study, wheat-D. breviaristatum 2Vb deletion lines were produced and identified by fluorescence in situ hybridization (FISH), and 74 molecular markers specific to D. breviaristatum chromosome 2Vb were physically localized in 4 distinct chromosomal regions. New wheat-D. breviaristatum 2Vb translocation lines were also characterized by FISH. The breakpoint of the translocation T3AS.3AL-2VbS was determined by physically mapped molecular markers. Field evaluation revealed that genes affecting plant height and spike length are located on fraction length (FL) 0.65-1.00 of 2VbS, while the stripe rust resistance gene(s) are located on FL 0.40-1.00 of D. breviaristatum chromosome 2VbL. The newly characterized wheat-Dasypyrum chromosomal introgressions are of potential value for the improvement of the yield and disease resistance of wheat.

A hexaploid triticale 4D (4B) substitution line confers superior stripe rust resistance

2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Houyang Kang ◽  
Yujie Wang ◽  
Chengdou Diao ◽  
Daiyan Li ◽  
Yi Wang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Hexaploid Triticale

scholarly journals Development and Molecular Cytogenetic Identification of a Novel Wheat–Leymus mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0140227 ◽  
Author(s):  
Xiaofei Yang ◽  
Changyou Wang ◽  
Xin Li ◽  
Chunhuan Chen ◽  
Zengrong Tian ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Molecular Cytogenetic ◽  
Leymus Mollis

Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance

Euphytica ◽  
2010 ◽  
Vol 177 (2) ◽  
pp. 169-177 ◽  
Author(s):  
Li-Jun Hu ◽  
Guang-Rong Li ◽  
Zi-Xian Zeng ◽  
Zhi-Jian Chang ◽  
Cheng Liu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Molecular Cytogenetic

scholarly journals Characterization of the Durum Wheat-Aegilops tauschii 4D(4B) Disomic Substitution Line YL-443 With Superior Characteristics of High Yielding and Stripe Rust Resistance

2021 ◽  
Vol 12 ◽  
Author(s):  
Fan Yang ◽  
Qier Liu ◽  
Qin Wang ◽  
Ning Yang ◽  
Jun Li ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Stripe Rust ◽  
Chromosome Pair ◽  
Rust Resistance ◽  
Aegilops Tauschii ◽  
Substitution Line ◽  
Yield Potential ◽  
High Yield ◽  
Stripe Rust Resistance ◽  
Pcr Marker

Durum wheat is one of the important food and cash crops. The main goals in current breeding programs are improving its low yield potential, kernel characteristics, and lack of resistance or tolerance to some biotic and abiotic stresses. In this study, a nascent synthesized hexaploid wheat Lanmai/AT23 is used as the female parent in crosses with its AB genome donor Lanmai. A tetraploid line YL-443 with supernumerary spikelets and high resistance to stripe rust was selected out from the pentaploid F7 progeny. Somatic analysis using multicolor fluorescence in situ hybridization (mc-FISH) revealed that this line is a disomic substitution line with the 4B chromosome pair of Lanmai replaced by the 4D chromosome pair of Aegilops tauschii AT23. Comparing with Lanmai, YL-443 shows an increase in the number of spikelets and florets per spike by 36.3 and 75.9%, respectively. The stripe rust resistance gene Yr28 carried on the 4D chromosome was fully expressed in the tetraploid background. The present 4D(4B) disomic substitution line YL-443 was distinguished from the previously reported 4D(4B) lines with the 4D chromosomes from Chinese Spring (CS). Our study demonstrated that YL-443 can be used as elite germplasm for durum wheat breeding targeting high yield potential and stripe rust resistance. The Yr28-specific PCR marker and the 4D chromosome-specific KASP markers together with its unique features of pubescent leaf sheath and auricles can be utilized for assisting selection in breeding.

Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139

2010 ◽  
Vol 36 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Hong ZHANG ◽  
Zhi-Long REN ◽  
Yin-Gang HU ◽  
Chang-You WANG ◽  
Wan-Quan JI
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Stripe Rust ◽  
Rust Resistance Genes
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