Paracrine Effects of Mesenchymal Stem Cells-Conditioned Medium on Microglial Cytokines Expression and Nitric Oxide Production

2014 ◽  
Vol 22 (4) ◽  
pp. 233-242 ◽  
Author(s):  
Yin Yin Ooi ◽  
S. Thameem Dheen ◽  
Samuel Sam Wah Tay
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Nitric Oxide Production ◽  
Paracrine Effects ◽  
Oxide Production

Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells

2009 ◽  
Vol 111 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Monia Orciani ◽  
Oriana Trubiani ◽  
Arianna Vignini ◽  
Monica Mattioli-Belmonte ◽  
R. Di Primio ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Comparison of TGF-β and Nitric Oxide Production by Adipose-Derived Mesenchymal Stem Cells Between Healthy Pregnant and Preeclamptic Women

2018 ◽  
pp. 11-20
Author(s):  
Ensie Sadat Mirsharif ◽  
Sakine Moaiedmohseni ◽  
Seyyed Mahmoud Hashemi ◽  
Eisa Salehi ◽  
Tooba Ghazanfari
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals SOCS1 Regulates the Immune Modulatory Properties of Mesenchymal Stem Cells by Inhibiting Nitric Oxide Production

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97256 ◽  
Author(s):  
Lei Zhang ◽  
Rui-Jie Dang ◽  
Hong Li ◽  
Ping Li ◽  
Yan-Mei Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Erythropoietin and Mesenchymal Stem Cells Properties

2020 ◽  
Vol 10 (5) ◽  
pp. 6197-6207
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adhesion Molecules ◽  
Erythropoietin Receptor ◽  
Proliferative Potential ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Autophagy Activity

The present work reports the effect of the long-term exposure bone marrow mesenchymal stem cells from chronic ischemic heart disease patients with erythropoietin on morpho-functional properties. The mesenchymal stem cells were grown under 3rd passage in medium supplemented with 33.4 IU/mL of recormon to study expression of adhesion molecules, erythropoietin receptor, cytokine receptor to common β-chain on surface, and proliferative potential (MTT test, CFU, cell cycle), migratory ability, nitric oxide production under growth factor deprivation, oxidative stress and hyperglycemia conditions, and autophagy activity, and ultrastructure changes in cells. The results showed that the long-term exposure mesenchymal stem cells with erythropoietin significantly increased proliferation, nitric oxide production and some adhesion molecules and receptors expression on surface of those cells. In addition, the erythropoietin long-term exposure with mesenchymal stem cells increases autophagy activity. The finding of this work indicates that erythropoietin could be used to augment cell resistance to adverse microenvironment condition.

scholarly journals Polyamines Modulate Nitric Oxide Production andCox-2Gene Expression in Response to Mechanical Loading in Human Adipose Tissue-Derived Mesenchymal Stem Cells

Stem Cells ◽  
2006 ◽  
Vol 24 (10) ◽  
pp. 2262-2269 ◽  
Author(s):  
Geuranne S. Tjabringa ◽  
Peter S. Vezeridis ◽  
Behrouz Zandieh-Doulabi ◽  
Marco N. Helder ◽  
Paul I.J.M. Wuisman ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Mechanical Loading ◽  
Human Adipose Tissue ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Genetically Engineered Mesenchymal Stem Cells that Overexpress ACE2 or Angiotensin‐(1–7) Show Enhanced Nitric‐Oxide Production

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Vinayak Shenoy ◽  
Meng Liu ◽  
Anandharajan Rathinasabapathy ◽  
Yanfei Qi ◽  
Michael J Katovich ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Genetically Engineered ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals Extracellular Vesicles from Adipose-Derived Mesenchymal Stem Cells Downregulate Senescence Features in Osteoarthritic Osteoblasts

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Miguel Tofiño-Vian ◽  
Maria Isabel Guillén ◽  
María Dolores Pérez del Caz ◽  
Miguel Angel Castejón ◽  
Maria José Alcaraz
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Extracellular Vesicles ◽  
Prostaglandin E ◽  
Protective Effects ◽  
Relevant Factor ◽  
Paracrine Effects ◽  
Inflammatory Stress ◽  
And Oxidative Stress

Osteoarthritis (OA) affects all articular tissues leading to pain and disability. The dysregulation of bone metabolism may contribute to the progression of this condition. Adipose-derived mesenchymal stem cells (ASC) are attractive candidates in the search of novel strategies for OA treatment and exert anti-inflammatory and cytoprotective effects on cartilage. Chronic inflammation in OA is a relevant factor in the development of cellular senescence and joint degradation. In this study, we extend our previous observations of ASC paracrine effects to study the influence of conditioned medium and extracellular vesicles from ASC on senescence induced by inflammatory stress in OA osteoblasts. Our results in cells stimulated with interleukin- (IL-) 1βindicate that conditioned medium, microvesicles, and exosomes from ASC downregulate senescence-associatedβ-galactosidase activity and the accumulation ofγH2AX foci. In addition, they reduced the production of inflammatory mediators, with the highest effect on IL-6 and prostaglandin E2. The control of mitochondrial membrane alterations and oxidative stress may provide a mechanism for the protective effects of ASC in OA osteoblasts. We have also shown that microvesicles and exosomes mediate the paracrine effects of ASC. Our study suggests that correction of abnormal osteoblast metabolism by ASC products may contribute to their protective effects.

scholarly journals Paracrine effects of mesenchymal stem cells in cisplatin-induced renal injury require heme oxygenase-1

2011 ◽  
Vol 300 (1) ◽  
pp. F254-F262 ◽  
Author(s):  
Abolfazl Zarjou ◽  
Junghyun Kim ◽  
Amie M. Traylor ◽  
Paul W. Sanders ◽  
József Balla ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Conditioned Medium ◽  
Heme Oxygenase ◽  
Morphological Changes ◽  
Heme Oxygenase 1 ◽  
Paracrine Effects ◽  
Angiogenic Potential ◽  
Promising Therapeutic Approach

Multipotent mesenchymal stem cells (MSC) have become a popular and promising therapeutic approach in many clinical conditions. MSC are beneficial in animal models of acute kidney injury (AKI), by mediating differentiation-independent paracrine properties, and have prompted ongoing clinical trials to evaluate the safety and efficacy of MSC. Heme oxygenase-1 (HO-1) is induced in response to stress including AKI and has important anti-apoptotic, anti-inflammatory, and proangiogenic properties in these settings. We therefore examined whether HO-1 plays a role in the beneficial effects of MSC in AKI. We isolated MSC from bone marrow of age-matched HO-1+/+ and HO-1−/− mice. Our studies indicate that while differentiation of MSC into osteo- and adipocytic lineages did not differ between cells isolated from HO-1+/+ and HO-1−/− mice, MSC from HO-1−/− mice had significantly lower angiogenic potential. Moreover, HO-1−/− MSC demonstrated reduced expression and secretion of several important growth and proangiogenic factors (stromal cell-derived factor-1, vascular endothelial growth factor-A, and hepatocyte growth factor) compared with MSC derived from HO-1+/+ mice. In addition, conditioned medium of HO-1+/+ MSC rescued functional and morphological changes associated with cisplatin-induced AKI, while the HO-1−/−-conditioned medium was ineffectual. Our studies indicate that HO-1 plays an important role in MSC-mediated protection. The results expand understanding of the renoprotective effects of MSC and may provide novel strategies to better utilize MSC in various disease models.

Sign in / Sign up

Export Citation Format

Share Document