Hydrogen Sulfide Ameliorates High-Glucose Toxicity in Rat Peritoneal Mesothelial Cells by Attenuating Oxidative Stress

2014 ◽  
Vol 126 (3) ◽  
pp. 157-165 ◽  
Author(s):  
Ying Lu ◽  
Huaying Shen ◽  
Xiaosong Shi ◽  
Sheng Feng ◽  
Zhi Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
High Glucose ◽  
Mesothelial Cells ◽  
Glucose Toxicity ◽  
Peritoneal Mesothelial Cells

scholarly journals Effect of β-(3,4-Dihydroxyphenyl) Lactic Acid on Oxidative Stress Stimulated by High Glucose Levels in Human Peritoneal Mesothelial Cells

2012 ◽  
Vol 40 (3) ◽  
pp. 943-953 ◽  
Author(s):  
H Zhang ◽  
J-W Wang ◽  
Y Xu ◽  
K Zhang ◽  
B Yi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lactic Acid ◽  
High Glucose ◽  
Mesothelial Cells ◽  
Time Dependent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Peritoneal Mesothelial Cells ◽  
Protein Levels ◽  
Glucose Levels ◽  
Human Peritoneal Mesothelial Cells

OBJECTIVE: To investigate the effects of β-(3,4-dihydroxyphenyl)lactic acid on oxidative stress stimulated by high glucose levels in human peritoneal mesothelial cells (HPMCs) in vitro. METHODS: HPMCs were incubated with 100 mol/l glucose followed by 0.625 – 20 mg/ml β-(3,4-dihydroxyphenyl)lactic acid. Reactive oxygen species (ROS) were quantified by flow cytometry. Relative levels of fibronectin-1 (FN1), collagen-I α1 (COL1A1), endothelin-1 (EDN1) and haem oxygenase-1 (HMOX1) mRNA and protein were quantified by real-time reverse transcription—polymerase chain reaction and Western blotting, respectively. Absolute levels of FN1 and COLIA1 were quantified by enzyme-linked immunosorbent assay. RESULTS: β-(3,4-Dihydroxyphenyl)lactic acid significantly decreased ROS levels, and EDN1 mRNA and protein levels, in dose- and time-dependent manners. HMOX1 mRNA and protein levels were significantly increased by β-(3,4-dihydroxyphenyl)lactic acid in dose-dependent manners. COL1A1 and FN1 mRNA and protein levels were significantly decreased by β-(3,4-dihydroxyphenyl)lactic acid in dose- and time-dependent manners. CONCLUSIONS: β-(3,4-Dihydroxyphenyl)lactic acid inhibited oxidative stress and reversed increases in FN1 and COLIA1 induced by high glucose levels in HPMCs. This may contribute to a protective role in peritoneal fibrosis.

scholarly journals High glucose levels inhibit focal adhesion kinase-mediated wound healing of rat peritoneal mesothelial cells

2003 ◽  
Vol 63 (2) ◽  
pp. 722-731 ◽  
Author(s):  
Masahito Tamura ◽  
Akihiko Osajima ◽  
Shingo Nakayamada ◽  
Hirofumi Anai ◽  
Narutoshi Kabashima ◽  
...  
Keyword(s):  
Wound Healing ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
High Glucose ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Glucose Levels

scholarly journals TMIGD1 Inhibited Abdominal Adhesion Formation by Alleviating Oxidative Stress in the Mitochondria of Peritoneal Mesothelial Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Yunhua Wu ◽  
Enmeng Li ◽  
Zijun Wang ◽  
Tianli Shen ◽  
Cong Shen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Function ◽  
Adhesion Formation ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Stress Injury ◽  
Peritoneal Mesothelial Cells ◽  
Abdominal Adhesion ◽  
Adhesion Tissue ◽  
Peritoneal Mesothelial Cell

Background. Postoperative abdominal adhesion remains one of the frequent complications after abdominal surgery and lacks effective intervention. Peritoneal mesothelial cell injury and healing play crucial roles in the process of adhesion formation, and identifying this mechanism might provide new insight into possible new therapeutic strategies for this disease. Transmembrane and immunoglobulin domain-containing 1 (TMIGD1) has been proven to protect renal epithelial cells from injury induced by oxidative stress and has also been identified as a novel adhesion molecule. Here, we investigated the role of TMIGD1 and its possible mechanism in adhesion formation. Materials and Methods. Immunohistochemistry (IHC), qPCR, and immunofluorescence (IHF) were used to detect the expression of TMIGD1. The grade and tenacity score of adhesion were used to evaluate the adhesion formation conditions. A TMIGD1-overexpressing HMrSV5 cell line was established. MTT assay, Western blotting, Annexin V apoptosis analysis, and CK19 staining were used to measure mesothelial cell viability, apoptosis, and completeness. ROS and MDA detection were used to measure mesothelial cell oxidative stress levels. JC-1 staining, IHF, and transmission electron microscopy were performed to assess mitochondrial function. Scratch-wound and adhesion assays were used to evaluate the adhesion ability of mesothelial cells. Results. First, we showed that TMIGD1 was decreased in mouse abdominal adhesion tissue and peritoneal mesothelial cells. Second, TMIGD1 overexpression inhibited adhesion formation. Third, TMIGD1 overexpression protected mesothelial cells from hydrogen peroxide- (H2O2-) induced oxidative stress injury. Fourth, TMIGD1 overexpression alleviated oxidative stress by protecting the mitochondrial function of mesothelial cells. In addition, TMIGD1 overexpression enhanced mesothelial cell adhesion. Conclusion. Our findings suggest that TMIGD1 protects mesothelial cells from oxidative stress injury by protecting their mitochondrial function, which is decreased in regular abdominal adhesion tissue. In addition, TMIGD1 enhances peritoneal mesothelial cell adhesion to promote healing.

The Role of Tgf-β in Growth Inhibition of Peritoneal Mesothelial Cells in High-Glucose Dialysate

1995 ◽  
Vol 15 (7_suppl) ◽  
pp. 93-95 ◽  
Author(s):  
Kazuo Kumano ◽  
Masuhiro Shimoda ◽  
Toru Hyodo ◽  
Tadasu Sakai
Keyword(s):  
Growth Inhibition ◽  
High Glucose ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells

scholarly journals High glucose promotes TGF-β1 production by inducing FOS expression in human peritoneal mesothelial cells

2015 ◽  
Vol 20 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Keiko Kokoroishi ◽  
Ayumu Nakashima ◽  
Shigehiro Doi ◽  
Toshinori Ueno ◽  
Toshiki Doi ◽  
...  
Keyword(s):  
High Glucose ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Human Peritoneal Mesothelial Cells ◽  
Fos Expression ◽  
Tgf Β1

scholarly journals GW25-e1408 Hydrogen sulfide ameliorates High glucose-induced senescence by suppressing oxidative stress

2014 ◽  
Vol 64 (16) ◽  
pp. C30
Author(s):  
Song Zhiming ◽  
Ma Xiaoju ◽  
Liu Yong ◽  
Hao Baoshun ◽  
Yu Shujie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
High Glucose
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