Preanalysis Storage Conditions Influence the Measurement of Brain-Derived Neurotrophic Factor Levels in Peripheral Blood

2014 ◽  
Vol 69 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Shoko Tsuchimine ◽  
Norio Sugawara ◽  
Masamichi Ishioka ◽  
Norio Yasui-Furukori
Keyword(s):  
Neurotrophic Factor ◽  
Peripheral Blood ◽  
Brain Derived Neurotrophic Factor ◽  
Storage Conditions

scholarly journals Brain-derived neurotrophic factor and autism: maternal and infant peripheral blood levels in the Early Markers for Autism (EMA) study

2008 ◽  
Vol 1 (2) ◽  
pp. 130-137 ◽  
Author(s):  
Lisa A. Croen ◽  
Paula Goines ◽  
Daniel Braunschweig ◽  
Robert Yolken ◽  
Cathleen K. Yoshida ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Peripheral Blood ◽  
Brain Derived Neurotrophic Factor ◽  
Blood Levels ◽  
Early Markers

scholarly journals Altered production of brain-derived neurotrophic factor by peripheral blood immune cells in multiple sclerosis

2010 ◽  
Vol 16 (10) ◽  
pp. 1178-1188 ◽  
Author(s):  
Satoshi Yoshimura ◽  
Hirofumi Ochi ◽  
Noriko Isobe ◽  
Takuya Matsushita ◽  
Kyoko Motomura ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Peripheral Blood Mononuclear Cells ◽  
Neurotrophic Factor ◽  
Peripheral Blood ◽  
Interferon Beta ◽  
Mononuclear Cells ◽  
Brain Derived Neurotrophic Factor ◽  
Peripheral Blood Mononuclear ◽  
Factor Production ◽  
Multiple Sclerosis Patients

Background: Within multiple sclerosis lesions, brain-derived neurotrophic factor is detected in neurons and immunocytes. Objective: To clarify brain-derived neurotrophic factor production by peripheral blood immunocytes and its relationship with clinical parameters in multiple sclerosis. Methods: Serum brain-derived neurotrophic factor levels were measured by conventional enzyme-linked immunosorbent assay while brain-derived neurotrophic factor production by immunocytes was determined by an in situ enzyme-linked immunosorbent assay in 74 multiple sclerosis patients, 32 healthy controls, and 86 patients with other neurological diseases. The tyrosine kinase receptor TrkB expression level in peripheral blood mononuclear cells was measured by real-time polymerase chain reaction. Results: Multiple sclerosis patients showed significantly higher serum brain-derived neurotrophic factor levels than healthy controls and patients with other neurological diseases. Multiple sclerosis patients with high brain-derived neurotrophic factor levels were younger, and showed fewer relapse numbers than those with low brain-derived neurotrophic factor levels. Brain-derived neurotrophic factor production by T cells increased with age in healthy controls, but not in multiple sclerosis patients. Interferon beta induced a significant increase in serum brain-derived neurotrophic factor levels. Brain-derived neurotrophic factor production from T cells and TrkB expression levels in peripheral blood mononuclear cells were significantly enhanced in interferon beta-treated multiple sclerosis patients compared with untreated ones. Conclusions: A high brain-derived neurotrophic factor level is related to early mild disease in young multiple sclerosis patients. Interferon beta potentiates brain-derived neurotrophic factor production and brain-derived neurotrophic factor receptor expression in peripheral blood mononuclear cells, which may act beneficially.

Sign in / Sign up

Export Citation Format

Share Document