scholarly journals Phospholipid Scramblase 1 Functionally Interacts with Angiogenin and Regulates Angiogenin-Enhanced rRNA Transcription

2013 ◽  
Vol 32 (6) ◽  
pp. 1695-1706 ◽  
Author(s):  
Junqiao Zhu ◽  
Jinghao Sheng ◽  
Haojie Dong ◽  
Lan Kang ◽  
Jian Ang ◽  
...  
Keyword(s):  
Phospholipid Scramblase ◽  
Rrna Transcription ◽  
Phospholipid Scramblase 1

scholarly journals Phospholipid Flip-Flop and Phospholipid Scramblase 1 (PLSCR1) Co-localize to Uropod Rafts in Formylated Met-Leu-Phe-stimulated Neutrophils

2004 ◽  
Vol 279 (17) ◽  
pp. 17625-17633 ◽  
Author(s):  
S. Courtney Frasch ◽  
Peter M. Henson ◽  
Kaz Nagaosa ◽  
Michael B. Fessler ◽  
Niels Borregaard ◽  
...  
Keyword(s):  
Flip Flop ◽  
Phospholipid Scramblase ◽  
Phospholipid Scramblase 1

Increased Expression of Phospholipid Scramblase 1 in Monocytes from Patients with Systemic Lupus Erythematosus

2010 ◽  
Vol 37 (8) ◽  
pp. 1639-1645 ◽  
Author(s):  
ERIKO SUZUKI ◽  
OLGA AMENGUAL ◽  
TATSUYA ATSUMI ◽  
KENJI OKU ◽  
TOKO HASHIMOTO ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Flow Cytometry ◽  
Cell Surface ◽  
Lupus Erythematosus ◽  
Splice Variants ◽  
Healthy Controls ◽  
Systemic Lupus ◽  
Phospholipid Scramblase ◽  
Phospholipid Scramblase 1 ◽  
D Dimer

Objective.A high incidence of thromboembolic events has been reported in patients with systemic lupus erythematosus (SLE). Phosphatidylserine (PS) is normally sequestered in the inner leaflet of cell membranes. Externalization of PS during cell activation is mediated by phospholipid scramblase 1 (PLSCR1) and has a central role in promoting blood coagulation. We investigated the underlying pathogenic status of thrombophilia in SLE by analyzing PLSCR1 expression on monocytes from patients with SLE.Methods.Sixty patients with SLE were evaluated. Twenty-three patients had antiphospholipid syndrome (APS/SLE). Plasma D-dimer levels were measured as a marker of fibrin turnover. The cDNA encoding human PLSCR1 was cloned from the total RNA extract from monocytes, and independent clones were sequenced. PLSCR1 mRNA expression in CD14+ cells was determined by real-time polymerase chain reaction. PS exposure on CD14+ cell surface was analyzed by flow cytometry.Results.Elevated D-dimer levels were found in plasma from SLE patients. Three splice variants of PLSCR1 mRNA were identified in all subjects, and levels of full-length PLSCR1 mRNA were significantly increased in SLE compared to healthy controls (2.9 ± 1.5 vs 1.3 ± 0.4, respectively; p < 0.0001). Flow-cytometry analysis showed relative enhancement of PS exposure in the surface of CD14+ cells in SLE patients compared to healthy controls.Conclusion.Novel PLSCR1 splice variants were identified. Monocytes in SLE patients had enhanced PLSCR1 mRNA expression, as well as increased fibrin turnover and cell-surface PS exposure, indicating that PLSCR1 may, in part, contribute to the prothrombotic tendency in SLE.

scholarly journals c-Abl Tyrosine Kinase Binds and Phosphorylates Phospholipid Scramblase 1

2001 ◽  
Vol 276 (31) ◽  
pp. 28984-28990 ◽  
Author(s):  
Jun Sun ◽  
Ji Zhao ◽  
Martin A. Schwartz ◽  
Jean Y. J. Wang ◽  
Therese Wiedmer ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Phospholipid Scramblase ◽  
Abl Tyrosine Kinase ◽  
Phospholipid Scramblase 1

scholarly journals Identification of Phospholipid Scramblase 1 as a Biomarker and Determination of Its Prognostic Value for Colorectal Cancer

2010 ◽  
Vol 17 (1-2) ◽  
pp. 41-47 ◽  
Author(s):  
Yung-Bin Kuo ◽  
Chung-Chuan Chan ◽  
C. Allen Chang ◽  
Chung-Wei Fan ◽  
Ray-Ping Hung ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Prognostic Value ◽  
Phospholipid Scramblase ◽  
Phospholipid Scramblase 1

GroES and GroEL are essential chaperones for refolding of recombinant human phospholipid scramblase 1 in E. coli

2009 ◽  
Vol 31 (11) ◽  
pp. 1745-1752 ◽  
Author(s):  
Santosh Kumar Sahu ◽  
Archita Rajasekharan ◽  
Sathyanarayana N. Gummadi
Keyword(s):  
E Coli ◽  
Phospholipid Scramblase ◽  
Phospholipid Scramblase 1

Abstract 1944: Phospholipid scramblase 1 and 3 as novel effectors of cripto-1 signaling

2011 ◽  
Author(s):  
Hideaki Karasawa ◽  
Tadahiro Nagaoka ◽  
Maria Cristina Rangel ◽  
Nadia P. Castro ◽  
Natalie Held ◽  
...  
Keyword(s):  
Phospholipid Scramblase ◽  
Phospholipid Scramblase 1

scholarly journals The Conformation of Human Phospholipid Scramblase 1, as Studied by Infrared Spectroscopy. Effects of Calcium and Detergent

2018 ◽  
Vol 114 (3) ◽  
pp. 403a
Author(s):  
Nagore Andraka ◽  
Lissete Sanchez-Magraner ◽  
Marcos Garcia-Pacios ◽  
Felix M. Goni ◽  
Jose L. Arrondo
Keyword(s):  
Infrared Spectroscopy ◽  
Phospholipid Scramblase ◽  
Phospholipid Scramblase 1

scholarly journals Engagement of Phospholipid Scramblase 1 in Activated Cells

2008 ◽  
Vol 283 (16) ◽  
pp. 10904-10918 ◽  
Author(s):  
Daniel Smrž ◽  
Pavel Lebduška ◽  
L'ubica Dráberová ◽  
Jan Korb ◽  
Petr Dráber
Keyword(s):  
Phospholipid Scramblase ◽  
Phospholipid Scramblase 1

Wogonoside Exerts Anti-Leukemia of AML Cells Via Restricting Phospholipid Scramblase 1 Gene Expression and Promoting Its Translocation Into Nuclear

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 4282-4282
Author(s):  
Yan Chen ◽  
Bao-An Chen ◽  
Qing-long Guo
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cell Lines ◽  
Underlying Mechanism ◽  
Phase Cell ◽  
Western Blots ◽  
Phospholipid Scramblase ◽  
Cycle Arrest ◽  
Phospholipid Scramblase 1

Abstract Abstract 4282 Objective: To evaluate the antileukemic effect of wogonoside and reveal the underlying mechanism. Method: In this study trypan blue dye exclusion assay, MTT assay, and soft agar colony formation assay were used to analysis growth inhibition of wogonoside the on AML (acute human promyelocytic) cell lines. Propidium iodide (PI)-staining and cell cycle-regulatory proteins detecting by western blots were applied to exam whether wogonoside could induce cell cycle arrest. Then a series of experiment were used to assess the ability of wogonoside to overcome the AML associated differentiation block, by using Giemsa staining, Nitroblue tetrazolium (NBT) reduction assay, and cell-surface differentiation antigens expression analysis. Real time PCR, western blots, cycloheximide inhibition test and RNA interference, nuclear and cytoplasmic fractionation, immunofluorescent staining were used to investigate the underlying mechanism. In this point we mainly focus that wogonoside exerts antileukemic by modulating of PLSCR1 gene expression, as well as influence its subcellular localization to play a role in regulating gene transcription. Result: It was demonstrated that wogonoside have the capacity to decrease the growth of myeloid cell lines by induction of G0/1 phase cell cycle arrest and differentiation. This effect is mediated by the increasing in mRNA and up-regulating protein expression of phospholipids scramblase 1 (PLSCR1). Meanwhile wogonoside promoted PLSCR1 traffic into the nucleus, which let PLSCR1 to play a role in regulating cell cycle and differentiation-related genes transcription including p21, p27, c-myc and IP3R1. Conclusion: Wogonoside induced AML cell lines to undergo differentiation and G1 phase arrest by restricting phospholipid scramblase 1 gene expression and promoting its translocation into nuclear. Disclosures: No relevant conflicts of interest to declare.

Sign in / Sign up

Export Citation Format

Share Document