scholarly journals Depressing Interleukin-1� Contributed to the Synergistic Effects of Tramadol and Minocycline on Spinal Nerve Ligation-Induced Neuropathic Pain

Neurosignals ◽  
2014 ◽  
Vol 22 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Xiao-Peng Mei ◽  
Yasushi Sakuma ◽  
Cheng Xie ◽  
Dan Wu ◽  
Ichinyo Ho ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Synergistic Effects ◽  
Interleukin 1 ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation

scholarly journals Tramadol and Propentofylline Coadministration Exerted Synergistic Effects on Rat Spinal Nerve Ligation-Induced Neuropathic Pain

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e72943 ◽  
Author(s):  
Jin Zhang ◽  
Dan Wu ◽  
Cheng Xie ◽  
Huan Wang ◽  
Wei Wang ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Synergistic Effects ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation

scholarly journals A Comparison of Surgical Invasions for Spinal Nerve Ligation with or without Paraspinal Muscle Removal in a Rat Neuropathic Pain Model

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yi-Gang Huang ◽  
Qing Zhang ◽  
Hao Wu ◽  
Chang-Qing Zhang
Keyword(s):  
Neuropathic Pain ◽  
Interleukin 1 ◽  
Physiological Model ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Paraspinal Muscle ◽  
Surgical Trauma ◽  
Physiological Processes ◽  
Neuropathic Pain Model ◽  
Removal Group

L5 spinal nerve ligation (SNL) in rats is one of the most popular models for studying neuropathic pain because of its high reproducibility. During the surgery, a part of the L5 paraspinal muscle is usually removed, which produces extra trauma and may potentially affect the physiological processes involved in neuropathic pain. To reduce the surgical trauma, the paraspinal muscle retraction was developed for exposure of the spinal nerve. The current study was aimed at comparing the surgical invasions between the L5 SNL models with paraspinal muscle removal or retraction. The results showed that both methods induced similar neuropathic pain behavior. However, the paraspinal muscle retraction group exhibited an average of 2.7 mg less blood loss than the muscle removal group. This group also showed a significantly lower increase in serum myoglobin and creatine phosphokinase levels on postoperative days 1 and 2, as well as a lower increase in interleukin-1βand interleukin-6 levels on postoperative day 1. The paraspinal muscle maintained normal morphological features following paraspinal muscle retraction. Our results indicate that the SNL rat model with paraspinal muscle retraction is a reliable physiological model that is reproducible, readily available, and less invasive than the model with muscle removal.

scholarly journals Three-dimensional analysis of the characteristics of joint motion and gait pattern in a rodent model following spinal nerve ligation

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Takayuki Seto ◽  
Hidenori Suzuki ◽  
Tomoya Okazaki ◽  
Yasuaki Imajo ◽  
Norihiro Nishida ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Pain Intensity ◽  
Rat Model ◽  
Gait Pattern ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Joint Motion ◽  
Behavioral Reactions ◽  
Post Operation ◽  
The Right

Abstract Background The spinal nerve ligation (SNL) rat is well known as the most common rodent model of neuropathic pain without motor deficit. Researchers have performed analyses using only the von Frey and thermal withdrawal tests to evaluate pain intensity in the rat experimental model. However, these test are completely different from the neurological examinations performed clinically. We think that several behavioral reactions must be observed following SNL because the patients with neuropathic pain usually have impaired coordination of the motions of the right–left limbs and right–left joint motion differences. In this study, we attempted to clarify the pain behavioral reactions in SNL rat model as in patients. We used the Kinema-Tracer system for 3D kinematics gait analysis to identify new characteristic parameters of each joint movement and gait pattern. Results The effect of SNL on mechanical allodynia was a 47 ± 6.1% decrease in the withdrawal threshold during 1–8 weeks post-operation. Sagittal trajectories of the hip, knee and ankle markers in SNL rats showed a large sagittal fluctuation of each joint while walking. Top minus bottom height of the left hip and knee that represents instability during walking was significantly larger in the SNL than sham rats. Both-foot contact time, which is one of the gait characteristics, was significantly longer in the SNL versus sham rats: 1.9 ± 0.15 s vs. 1.03 ± 0.15 s at 4 weeks post-operation (p = 0.003). We also examined the circular phase time to evaluate coordination of the right and left hind-limbs. The ratio of the right/left circular time was 1.0 ± 0.08 in the sham rats and 0.62 ± 0.15 in the SNL rats at 4 weeks post-operation. Conclusions We revealed new quantitative parameters in an SNL rat model that are directly relevant to the neurological symptoms in patients with neuropathic pain, in whom the von Frey and thermal withdrawal tests are not used at all clinically. This new 3D analysis system can contribute to the analysis of pain intensity of SNL rats in detail similar to human patients’ reactions following neuropathic pain.

scholarly journals Nefopam downregulates autophagy and c-Jun N-terminal kinase activity in the regulation of neuropathic pain development following spinal nerve ligation

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Seon-Hee Oh ◽  
Myung Ha Yoon ◽  
Kyung Joon Lim ◽  
Byung Sik Yu ◽  
In Gook Jee ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Kinase Activity ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation

Comprehensive Analysis of Differentially Expressed Genes and Key Pathways in Neuropathic Pain by Spinal Nerve Ligation

2020 ◽  
Author(s):  
Chao Xu ◽  
HuiFang Li ◽  
YunPeng Zhang ◽  
TianYu Liu ◽  
Yi Feng
Keyword(s):  
Functional Analysis ◽  
Neuropathic Pain ◽  
Differentially Expressed Genes ◽  
Enrichment Analysis ◽  
Gene Expression Omnibus ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Differentially Expressed ◽  
Hub Genes ◽  
Long Term Treatment

Abstract Background: Neuropathic pain can cause significant physical and economic burden to people, and there are no effective long-term treatment methods for this condition. We conducted a bioinformatics analysis of microarray data to identify related mechanisms to determine strategies for more effective treatments of neuropathic pain.Methods: GSE24982 and GSE63442 microarray datasets were extracted from the Gene Expression Omnibus (GEO) database to analyze transcriptome differences of neuropathic pain in the dorsal root ganglions caused by spinal nerve ligation. We filtered the differentially expressed genes (DEGs) in the two datasets and Webgestalt was applied to conduct GeneOntology (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the shared DEGs. String Database and Cytoscape software were used to construct the Protein-Protein Interaction (PPI) network to determine the hub genes, which were subsequently verified in the GSE30691 dataset. Finally, miRDB and miRWalk Databases were used to predict potential miRNA of the selected DEGs.Results: A total of 182 overlapped DEGs were found between GSE24982 and GSE63442 datasets. The GO functional analysis and KEGG enrichment analysis showed that the selected DEGs were mainly enriched in infection, transmembrane transport of ion channels, and synaptic transmission. Combining the results of PPI analysis and the verification of the GSE30691 dataset, we identified seven hub genes related to neuropathic pain (Atf3, Aif1, Ctss, Gfap, Scg2, Jun, and Vgf). Predicted miRNA targeting each selected hub genes were identified.Conclusion: Seven hub genes related to the pathogenesis of neuropathic pain and potential targeting miRNA were identified, expanding understanding of the mechanism of neuropathic pain and facilitating treatment development.

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