Efficacy of CytoLyt® Hemolytic Action on ThinPrep® LBC Using Cultured Osteosarcoma Cell Line LM8

2014 ◽  
Vol 58 (1) ◽  
pp. 76-82 ◽  
Author(s):  
Yoshiaki Norimatsu ◽  
Hiroyuki Ohsaki ◽  
Hiroshi Masuno ◽  
Akihiro Kagawa ◽  
Norihiro Teramoto ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Hemolytic Action

scholarly journals VCP (p97) Regulates NFKB Signaling Pathway, Which Is Important for Metastasis of Osteosarcoma Cell Line

2002 ◽  
Vol 93 (3) ◽  
pp. 296-304 ◽  
Author(s):  
Tatsuya Asai ◽  
Yasuhiko Tomita ◽  
Shin-ichi Nakatsuka ◽  
Yoshihiko Hoshida ◽  
Akira Myoui ◽  
...  
Keyword(s):  
Cell Line ◽  
Signaling Pathway ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell

Recognition by cellular and humoral autologous immunity in a human osteosarcoma cell line

2003 ◽  
Vol 8 (4) ◽  
pp. 554-559 ◽  
Author(s):  
Yuki Nabeta ◽  
Kawaguchi Satoshi ◽  
Hiroeki Sahara ◽  
Hideyuki Ikeda ◽  
Yoshihiko Hirohashi ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

LRP8 activates STAT3 to induce PD-L1 expression in osteosarcoma

2020 ◽  
pp. 030089162095287
Author(s):  
Shiqin Zheng ◽  
Yuxi Wei ◽  
Yu Jiang ◽  
Yi Hao
Keyword(s):  
Cell Line ◽  
Culture System ◽  
Nuclear Translocation ◽  
Interleukin 2 ◽  
Dominant Negative ◽  
Jurkat Cells ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Normal Tissues

Purpose: Targeting programmed death-ligand 1 (PD-L1) may be an effective intervention for osteosarcoma and PD-L1 expression is controlled by diverse regulatory factors. Low-density lipoprotein receptor-related protein 8 (LRP8) regulates osteoblast differentiation and it is unclear whether and how LRP8 could contribute to osteosarcoma pathogenesis. In this study, we investigated the LRP8/signal transducer and activator of transcription 3 (STAT3)/PD-L1 network in osteosarcoma. Methods: The expression of LRP8, STAT3, and PD-L1 was measured in osteosarcoma tissues and paired normal tissues. The effects of LRP8 on STAT3 and PD-L1 expression were investigated in an osteosarcoma cell line. The effects on immunosuppression were investigated in an in vitro co-culture system with Jurkat cell line and osteosarcoma cell line. The effects of LRP8 were blocked by a LRP8 neutralizing antibody, dominant-negative STAT3, or STAT3 inhibitor. Results: LRP8 was overexpressed in osteosarcoma compared to normal tissues and its level was correlated with phospho-STAT3 (p-STAT3) level in osteosarcoma tissues. In osteosarcoma cell lines, LRP8 increased p-STAT3 level and promoted nuclear translocation of STAT3. STAT3 activation also increased PD-L1 mRNA, protein, and promoter activity. In addition, LRP8 enhanced PD-L1 expression via STAT3. In a co-culture system, LRP8 overexpression in an osteosarcoma cell line impaired viability and interleukin-2 secretion of Jurkat cells and induced apoptosis of Jurkat cells. The effects of LRP8 could be blocked by neutralizing LRP8 antibody or STAT3 inhibitor. Blocking LRP8 inhibits proliferation and induces apoptosis of osteosarcoma cells. Conclusions: Our results provide evidence for a novel regulation network of LRP8/STAT3/PD-L1 in osteosarcoma and LRP8 may be a potential therapeutic target in osteosarcoma.

Inhibitory Effects of Indomethacin in Human MNNG/HOS Osteosarcoma Cell Line In Vitro

2019 ◽  
Vol 38 (1) ◽  
pp. 23-36 ◽  
Author(s):  
Saied Mirshahidi ◽  
Rosalia de Necochea-Campion ◽  
Annie Moretta ◽  
Nadine L. Williams ◽  
Mark E. Reeves ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Inhibitory Effects
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