Characterization of the Reproductive Effects of theVgf-Derived Peptide TLQP-21 in Female Rats: In vivo and in vitro Studies

2013 ◽  
Vol 98 (1) ◽  
pp. 38-50 ◽  
Author(s):  
Enrique Aguilar ◽  
Rafael Pineda ◽  
Francisco Gaytán ◽  
Miguel A. Sánchez-Garrido ◽  
Magdalena Romero ◽  
...  
Keyword(s):  
In Vitro Studies ◽  
Female Rats ◽  
Reproductive Effects

Inhibition of LH-stimulated cyclic AMP formation in pre-ovulatory rat granulosa cells by follicular fluid

1980 ◽  
Vol 95 (1) ◽  
pp. 84-89 ◽  
Author(s):  
Knut Nordenström ◽  
Anita Sjögren ◽  
Lars Hamberger
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Phosphodiesterase Inhibitor ◽  
Inhibitory Effect ◽  
Female Rats ◽  
Bicarbonate Buffer ◽  
Ovulatory Follicles

Abstract. Immature female rats were injected sc with a single dose of PMSG to induce growth and maturation of ovarian follicles. In the morning of prooestrus the rats were given a single ip injection of LH (10 μg/rat) or 0.154 m NaCl, 2 h prior to sacrifice. Granulosa cells were isolated from the pre-ovulatory follicles and incubated in Krebs bicarbonate buffer, for 1 h with or without in vitro addition of various test substances. Following incubation the amounts of cAMP in tissue plus medium were determined. It was found that the isolated granulosa cells exposed to LH in vivo responded to the addition of LH in vitro with a production of high amounts of cAMP, i.e. these cells were not refractory to LH stimulation and in fact responded better than granulosa cells isolated from ovaries not exposed to LH in vivo. The addition to the incubation medium of follicular fluid (FFl) obtained from pre-ovulatory follicles decreased the effect of LH in vitro when added at a final concentration of 1% and completely abolished it at a concentration of 3%. Removal of steroids from the FFl did not influence the inhibitory effect and the addition of a phosphodiesterase inhibitor (IBMX) in vitro did not alter the results in principle. These results point to the existence of a factor in the FF1 which interacts with the sensitivity of the isolated preovulatory granulosa cells to repeated exposures to LH. Characterization of this factor is subject to further investigations.

Effects of Ghrelin upon Gonadotropin-Releasing Hormone and Gonadotropin Secretion in Adult Female Rats: In vivo and in vitro Studies

2005 ◽  
Vol 82 (5-6) ◽  
pp. 245-255 ◽  
Author(s):  
Rafael Fernández-Fernández ◽  
Manuel Tena-Sempere ◽  
Víctor M. Navarro ◽  
María L. Barreiro ◽  
Juan M. Castellano ◽  
...  
Keyword(s):  
Adult Female ◽  
Gonadotropin Releasing Hormone ◽  
In Vitro Studies ◽  
Female Rats ◽  
Gonadotropin Secretion ◽  
Releasing Hormone

Characterization of Recombinantly Expressed Rat and Monkey Intestinal Alkaline Phosphatases: In Vitro Studies and In Vivo Correlations

2013 ◽  
Vol 41 (7) ◽  
pp. 1425-1432 ◽  
Author(s):  
Murali Subramanian ◽  
Sundeep Paruchury ◽  
Shashyendra Singh Gautam ◽  
Sheelendra Pratap Singh ◽  
Rambabu Arla ◽  
...  
Keyword(s):  
In Vitro Studies ◽  
Alkaline Phosphatases

IN VITRO STUDIES ON COLLAGEN METABOLISM IN METAPHYSEAL RAT BONE

1975 ◽  
Vol 80 (4) ◽  
pp. 784-794 ◽  
Author(s):  
Norvald Langeland ◽  
Vigdis Teig
Keyword(s):  
Incorporation Rate ◽  
In Vitro Studies ◽  
Collagen Metabolism ◽  
Female Rats ◽  
Bone Collagen ◽  
Metaphyseal Bone ◽  
Rat Bone

ABSTRACT In vitro collagen metabolism in metaphyseal bone has been studied during the first few hours and days after the start of in vivo treatment of castrated female rats with various doses of oestradiol-17β. Already during the first 2 h after the start of treatment with oestradiol-17β 2 μg/day and within the first 24 h in the rats receiving 20 μg/day, a reduction of the collagen incorporation rate was demonstrated. The incorporation rates remained at approximately the same reduced level during the following 3 weeks. In rats receiving oestradiol-17β 1 μg/day no such reduced incorporation rate could be demonstrated during the first 36 h after the start of treatment. The bone collagen resorption rate was significantly elevated during the first 3 days of treatment in the animals receiving oestradiol-17β both 1 and 20 μg/day. After this time the resorption rates gradually fell to levels significantly below controls at 3 weeks after start of treatment.

Characterization of insulin degradation products generated in liver endosomes: In vivo and in vitro studies

1990 ◽  
Vol 72 (3) ◽  
pp. 175-185 ◽  
Author(s):  
Jean-Pierre Clot ◽  
Michel Janicot ◽  
Françoise Fouque ◽  
Bernard Desbuquois ◽  
Pierre-Yves Haumont ◽  
...  
Keyword(s):  
Degradation Products ◽  
In Vitro Studies ◽  
Insulin Degradation
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