scholarly journals Amphipaths Differentially Modulate Membrane Surface Deformation in Rat Peritoneal Mast Cells During Exocytosis

2013 ◽  
Vol 31 (4-5) ◽  
pp. 592-600 ◽  
Author(s):  
Itsuro Kazama ◽  
Yoshio Maruyama ◽  
Sara Takahashi ◽  
Takashi Kokumai
Keyword(s):  
Mast Cells ◽  
Surface Deformation ◽  
Membrane Surface ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

scholarly journals Olopatadine Inhibits Exocytosis in Rat Peritoneal Mast Cells by Counteracting Membrane Surface Deformation

2015 ◽  
Vol 35 (1) ◽  
pp. 386-396 ◽  
Author(s):  
Asuka Baba ◽  
Masahiro Tachi ◽  
Yoshio Maruyama ◽  
Itsuro Kazama
Keyword(s):  
Plasma Membrane ◽  
Mast Cells ◽  
Lipid Bilayers ◽  
Surface Deformation ◽  
Lucifer Yellow ◽  
Membrane Surface ◽  
Water Soluble ◽  
Confocal Imaging ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

Backgroud/Aims: Besides its anti-allergic properties as a histamine receptor antagonist, olopatadine stabilizes mast cells by inhibiting the release of chemokines. Since olopatadine bears amphiphilic features and is preferentially partitioned into the lipid bilayers of the plasma membrane, it would induce some morphological changes in mast cells and thus affect the process of exocytosis. Methods: Employing the standard patch-clamp whole-cell recording technique, we examined the effects of olopatadine and other anti-allergic drugs on the membrane capacitance (Cm) in rat peritoneal mast cells during exocytosis. Using confocal imaging of a water-soluble fluorescent dye, lucifer yellow, we also examined their effects on the deformation of the plasma membrane. Results: Low concentrations of olopatadine (1 or 10 µM) did not significantly affect the GTP-γ-S-induced increase in the Cm. However, 100 µM and 1 mM olopatadine almost totally suppressed the increase in the Cm. Additionally, these doses completely washed out the trapping of the dye on the cell surface, indicating that olopatadine counteracted the membrane surface deformation induced by exocytosis. As shown by electron microscopy, olopatadine generated inward membrane bending in mast cells. Conclusion: This study provides electrophysiological evidence for the first time that olopatadine dose-dependently inhibits the process of exocytosis in rat peritoneal mast cells. Such mast cell stabilizing properties of olopatadine may be attributed to its counteracting effects on the plasma membrane deformation in degranulating mast cells.

scholarly journals Native heparin from rat peritoneal mast cells.

1977 ◽  
Vol 252 (2) ◽  
pp. 518-521 ◽  
Author(s):  
R W Yurt ◽  
R W Leid ◽  
K F Austen
Keyword(s):  
Mast Cells ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

The Potencies of Thapsigargin and Analogues as Activators of Rat Peritoneal Mast Cells

Planta Medica ◽  
1986 ◽  
Vol 52 (04) ◽  
pp. 251-255 ◽  
Author(s):  
Elsebeth Norup ◽  
Ulla Smitt ◽  
S. Christensen
Keyword(s):  
Mast Cells ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells
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