Reduced Glucocorticoid Receptor Protein Expression in Children with Critical Illness

2013 ◽  
Vol 79 (3) ◽  
pp. 169-178 ◽  
Author(s):  
Justin A. Indyk ◽  
Cristina Candido-Vitto ◽  
Irene M. Wolf ◽  
Shekhar Venkataraman ◽  
Ricardo Munoz ◽  
...  
Keyword(s):  
Critical Illness ◽  
Glucocorticoid Receptor ◽  
Protein Expression ◽  
Receptor Protein

scholarly journals Glucocorticoid receptor protein expression in human hippocampus; stability with age

2013 ◽  
Vol 34 (6) ◽  
pp. 1662-1673 ◽  
Author(s):  
Qian Wang ◽  
Joop Van Heerikhuize ◽  
Eleonora Aronica ◽  
Mitsuhiro Kawata ◽  
Laszlo Seress ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Protein Expression ◽  
Receptor Protein ◽  
Human Hippocampus

Decreased glucocorticoid receptor expression during critical illness

2019 ◽  
Vol 49 (4) ◽  
pp. e13073 ◽  
Author(s):  
Alice G. Vassiliou ◽  
Georgios Floros ◽  
Edison Jahaj ◽  
Georgios Stamogiannos ◽  
Sofianna Gennimata ◽  
...  
Keyword(s):  
Critical Illness ◽  
Glucocorticoid Receptor ◽  
Receptor Expression

Electroacupuncture inhibits the corneal ROS/TXNIP/NLRP3 signaling pathway in a rat model of dry eye syndrome

2021 ◽  
pp. 096452842110392
Author(s):  
Yanting Yang ◽  
Dan Zhang ◽  
Lijie Wu ◽  
Ji Zhang ◽  
Danyan Wu ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Dry Eye ◽  
Clinical Symptoms ◽  
Dry Eye Syndrome ◽  
Receptor Protein ◽  
Sprague Dawley ◽  
Corneal Injury ◽  
Caspase 1 ◽  
Des Model

Background: Electroacupuncture (EA) treatment has been found to ameliorate clinical symptoms in patients with dry eye, but its mechanisms are still not entirely clear. Objective: To study the regulation of EA on ocular surface function and the corneal reactive oxygen species (ROS)/thioredoxin-interacting protein (TXNIP)/Nod-like receptor protein 3 (NLRP3) inflammatory signaling pathway in dry eye syndrome (DES) model rats. Methods: Male Sprague-Dawley (SD) rats were randomly divided into five groups: Normal, Model, Model + EA, Model + NAC (N-actetylcysteine) and Model + NS (normal saline). The DES model was developed by subcutaneous injection of scopolamine hydrobromide with exposure to an air draft in the latter four groups. After intervention, the Schirmer I test (SIT), tear film break-up time (BUT) and ROS content were measured, the histopathological changes of corneal tissues were observed, and the mRNA and protein expression levels of TXNIP, NLRP3, apoptosis-associated Speck-like protein containing CARD (ASC), caspase-1, interleukin (IL)-1β and IL-18 were detected. Results: Compared with the Model group, the SIT and BUT increased significantly in the Model + EA group after intervention (p < 0.05), and the corneal injury was improved. Corneal ROS content declined in both Model + EA and Model + NAC groups (p < 0.05), and mRNA expression of TXNIP, NLRP3, ASC and caspase-1 also decreased (p < 0.01). Corneal protein expression of TXNIP, NLRP3, IL-1β and IL-18 decreased significantly in the Model + EA group (p < 0.01). Conclusion: Inhibiting the ROS/TXNIP/NLRP3 signaling pathway may be the mechanism underlying the role of EA in improving corneal injury in DES model rats.

scholarly journals Thyrotrophin receptor protein expression in normal and adenomatous human pituitary

2000 ◽  
Vol 167 (1) ◽  
pp. 7-13 ◽  
Author(s):  
M Theodoropoulou ◽  
T Arzberger ◽  
Y Gruebler ◽  
Z Korali ◽  
P Mortini ◽  
...  
Keyword(s):  
Protein Expression ◽  
Pituitary Adenomas ◽  
Anterior Pituitary ◽  
Preliminary Evidence ◽  
Positive Control ◽  
Negative Control ◽  
Receptor Protein ◽  
Human Pituitary ◽  
Thyrotrophin Releasing Hormone ◽  
Regulatory Pathways

Thyrotrophin (TSH) synthesis and secretion is under the positive control of thyrotrophin releasing hormone and under the negative control of the thyroid hormones. However, it is hypothesised that TSH has a direct effect on the regulation of its own synthesis through an intrapituitary loop mediated by pituitary TSH receptors (TSH-R). The aim of this investigation was to study the expression of TSH-R in normal human pituitary at mRNA and protein levels, and to compare the pattern of protein expression between different pituitary adenomas. Using RT-PCR we were able to detect TSH-R mRNA in the normal pituitary, and immunohistochemical studies showed TSH-R protein expression in distinct areas of the anterior pituitary. Double immunostaining with antibodies against each of the intrapituitary hormones and S100 revealed that TSH-R protein is present in thyrotrophs and folliculostellate cells. Examination of 58 pituitary adenomas, including two clinically active and two clinically inactive thyrotroph adenomas, revealed TSH-R immunopositivity in only the two clinically inactive thyrotroph adenomas. This study shows, for the first time, the presence of TSH-R protein in the normal anterior pituitary and in a subset of thyrotroph adenomas. The expression of TSH-R in the thyrotroph and folliculostellate cell subpopulations provides preliminary evidence of a role for TSH in autocrine and paracrine regulatory pathways within the anterior pituitary gland.

scholarly journals Acute bout of resistance exercise increases vitamin D receptor protein expression in rat skeletal muscle

2015 ◽  
Vol 100 (10) ◽  
pp. 1168-1176 ◽  
Author(s):  
Yuhei Makanae ◽  
Riki Ogasawara ◽  
Koji Sato ◽  
Yusuke Takamura ◽  
Kenji Matsutani ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Protein Expression ◽  
Resistance Exercise ◽  
Vitamin D Receptor ◽  
Receptor Protein ◽  
Rat Skeletal Muscle ◽  
Acute Bout
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