Comparative Study of the Effects of Recombinant Human Epidermal Growth Factor and Basic Fibroblast Growth Factor on Corneal Epithelial Wound Healing and Neovascularization in vivo and in vitro

2013 ◽  
Vol 49 (3) ◽  
pp. 150-160 ◽  
Author(s):  
Limeng Yan ◽  
Wei Wu ◽  
Zhichong Wang ◽  
Chaoyang Li ◽  
Xiaohe Lu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Corneal Epithelial ◽  
Epithelial Wound Healing ◽  
Epidermal Growth

scholarly journals ENHANCEMENT OF WOUND HEALING BY TOPICAL APPLICATION OF EPIDERMAL GROWTH FACTOR IN ANIMAL MODEL

2020 ◽  
Author(s):  
George Zhu ◽  
Xu HL ◽  
Zhou XP ◽  
Zhi QW
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Peer Review ◽  
Healing Time ◽  
Epidermal Growth ◽  
Review Reports ◽  
E Mail

Objective: Wound healing is a complex process of biological events involving re-epithelialization and granulation that are mainly mediated by several endogenously released growth factors such as epidermal growth factor. This work was undertaken to study the effects of various doses of locally applied recombinant human epidermal growth factor (rhEGF) on wound healing in rats. Methods: Recombinant human EGF consists of 53 amino acids. In vitro, rhEGF promoted it’s obvious cell growth and proliferation when added to cultured 3T3 cells using MTT assay. In the test groups, in vivo, wound sites were given daily with a solution containing 2, 5, 10, 50ug of EGF spray and 40ug of EGF ointment, respectively. We presented evidence that a significant decreased healing time in wound was observed in all rhEGF groups when compared with the control, and reach to its maximal efficacy at 10ug/ml of rhEGF spray. Results: The rate of wound closure was over 50 per cent at initial 3 days of treatment. Treatment with rhEGF significantly decreased the length of time to over 50 per cent healing by approximately 4-5 days, and that to 70 per cent and 90 per cent healing by approximately 3-4 days and 3 days, respectively. A stimulatory, dose-dependent effect of EGF on wound healing was observed with increased hEGF concentration. In toxicological group, higher doses of 100ug/ml of rhEGF spray was applied by local dorsal incision in rats. Moreover, a dose of single 200ug, single 300ug or 300ug within 24 hours of subcutaneous and intramuscular rhEGF injection was given respectively. There were no significant adverse side effects.Conclusion: Current study recommended a proposal of clinical drug doses in wound at 2µg, 5 µg and 10 µg /ml of rhEGF spray, and 10 µg and even higher 40 µg rhEGF/g of ointment. The results indicated that prepared rhEGF by our genetic engineering is safe, and is emerging in clinical effective use in assisting wound healing time. Peer Review History: Received 8 January 2020;   Revised 12 February; Accepted 2 March, Available online 15 March 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.5/10 Average Peer review marks at publication stage: 8.5/10 Reviewer(s) detail: Name: Asmaa Ahmed Mohamed Ahmed Khalifa   Affiliation: Pharos University, Alexandria, Egypt E-mail: [email protected]   Name: Dr. Sabah Hussien El-Ghaiesh  Affiliation: Tanta University, Egypt E-mail: [email protected] Comments of reviewer(s): Similar Articles: POTENTIAL OF SNAKEHEAD FISH (OPHIOCEPHALUS STRIATUS) IN ACCELERATING WOUND HEALING

scholarly journals Ixora coccinea Enhances Cutaneous Wound Healing by Upregulating the Expression of Collagen and Basic Fibroblast Growth Factor

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Aadesh Upadhyay ◽  
Pronobesh Chattopadhyay ◽  
Danswrang Goyary ◽  
Papiya Mitra Mazumder ◽  
Vijay Veer
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Western Blot ◽  
Fibroblast Growth Factor ◽  
Methanol Extract ◽  
Fibroblast Growth ◽  
Fibroblast Proliferation ◽  
Ixora Coccinea

Background. Ixora coccinea L. (Rubiaceae) has been documented for traditional use in hypertension, menstrual irregularities, sprain, chronic ulcer, and skin diseases. In the present study, I. coccinea was subjected to in vitro and in vivo wound healing investigation. Methods. Petroleum ether, chloroform, methanol, and water sequential I. coccinea leaves extracts were evaluated for in vitro antioxidant, antimicrobial, and fibroblast proliferation activities. The promising I. coccinea methanol extract (IxME) was screened for in vivo wound healing activity in Wistar rat using circular excision model. Wound contraction measurement, hydroxyproline quantification, and western blot for collagen type III (COL3A1), basic fibroblast growth factor (bFGF), and Smad-2, -3, -4, and -7 was performed with 7-day postoperative wound granulation tissue. Gentamicin sulfate (0.01% w/w) hydrogel was used as reference standard. Results. IxME showed the potent antimicrobial, antioxidant activities, with significant fibroblast proliferation inducing activity, as compared to all other extracts. In vivo study confirmed the wound healing accelerating potential of IxME, as evidenced by faster wound contraction, higher hydroxyproline content, and improved histopathology of granulation tissue. Western blot analysis revealed that the topical application of I. coccinea methanol extract stimulates the fibroblast growth factor and Smad mediated collagen production in wound tissue.

The in vivo effects of fibroblast growth factor and epidermal growth factor on the secretion of oestradiol, androstenedione and progesterone by the autotransplanted ovary in the ewe

1995 ◽  
Vol 146 (2) ◽  
pp. 301-311 ◽  
Author(s):  
R J Scaramuzzi ◽  
J A Downing
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Fibroblast Growth Factor ◽  
Detectable Effect ◽  
Fibroblast Growth ◽  
Luteal Function ◽  
Indirect Consequence ◽  
Epidermal Growth ◽  
Lh Secretion

Abstract An experiment was conducted to determine the effects of epidermal growth factor (EGF) and fibroblast growth factor (FGF), infused into the ovarian artery, on the secretion of ovarian steroids during the mid-luteal phase in ewes with an autotransplanted ovary. The infusion of EGF (5 μg/h) for 12 h suppressed the secretion of oestradiol and androstenedione during the infusion and for up to 30 h after the infusion. The secretion of progesterone tended to be lower immediately after the infusion (not significant) but had recovered by 24 h after the end of the infusion and then increased significantly (P<0·05) to rates higher than in control animals. There were no effects of the infusion of EGF on the characteristics of pulsatile LH secretion. FSH concentrations increased 24 h after the end of the infusion probably as an indirect consequence of the changes in oestradiol secretion and not as a consequence of a direct effect of EGF on the hypothalamo-pituitary axis although this latter possibility cannot be unequivocally eliminated. The infusion of FGF (1·5 μg/h) for 12 h also suppressed the secretion of oestradiol and androstenedione during and for up to 30 h after the infusion. The infusion of FGF had no detectable effect on the secretion of progesterone or the characteristics of pulsatile LH secretion. FSH concentrations increased steadily during the infusion but declined rapidly to below pre-infusion concentrations after the end of the infusion. These data provide tentative in vivo evidence for paracrine and autocrine effects of EGF and FGF on follicular and luteal function in sheep. Journal of Endocrinology (1995) 146, 301–311

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