scholarly journals Effects of Ghrelin on the Intracellular Calcium Concentration in Rat Aorta Vascular Smooth Muscle Cells

2012 ◽  
Vol 30 (5) ◽  
pp. 1299-1309 ◽  
Author(s):  
Huan Fang ◽  
Zhen Hong ◽  
Jun Zhang ◽  
Dai-Fei Shen ◽  
Fen-Fei Gao ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Intracellular Calcium ◽  
Vascular Smooth Muscle Cells ◽  
Calcium Concentration ◽  
Muscle Cells ◽  
Rat Aorta ◽  
Intracellular Calcium Concentration

Relationship between extra and intracellular sources of calcium and the contractile effect of thiopental in rat aorta

2001 ◽  
Vol 79 (5) ◽  
pp. 407-414 ◽  
Author(s):  
Carlos Castillo Henkel ◽  
Juan Asbun ◽  
Guillermo Ceballos ◽  
María del Carmen Castillo ◽  
Enrique F Castillo
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Muscle Cells ◽  
Rat Aorta ◽  
Calcium Entry ◽  
Intracellular Calcium Concentration ◽  
Free Solution ◽  
Contractile Effect

To evaluate the relationship between the vasocontractile effect of thiopental and the extra and intracellular sources of Ca2+, we analyzed both the contractile effect of the barbiturate on rat aortic rings and its ability to modify the intracellular calcium concentration in cultured rat aorta smooth muscle cells. Thiopental (10–310 µg/mL) contracted aortic rings only in the presence of extracellular Ca2+, and this effect was not blocked by verapamil or diltiazem. On the contrary, Ca2+ (0.1–3.1 mM) evoked contractions only when thiopental (100 µg/mL) was present. Although in calcium-free solution thiopental (100 µg/mL) did not contract aortic rings, it abolished the contractile effect of either phenylephrine (10–6 M) or caffeine (10 mM). Finally, thiopental augmented the intracellular calcium concentration in cultured smooth muscle cells incubated either in the presence or absence of calcium. In conclusion, thiopental's vasocontractile effect depends on extracellular calcium influx, which is independent of L-calcium channels. The increase in intracellular Ca2+ concentration elicited by thiopental in Ca2+-free solution and its ability to block the effect of phenylephrine and caffeine suggest that this barbiturate can deplete intracellular pools of calcium. Therefore, the calcium entry pathway associated with the contractile effect of thiopental may correspond to the capacitative calcium entry model.Key words: smooth muscle, Ca2+ kinetics, vasoconstriction.

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