Primordial Germ Cells (Spermatogonial Stem Cells) of Bullfrogs Express Sex Hormone-Binding Globulin and Steroid Receptors during Seasonal Spermatogenesis

2013 ◽  
Vol 197 (2) ◽  
pp. 136-144 ◽  
Author(s):  
Breno Henrique Caneguim ◽  
Flávia Luciana Beltrame ◽  
Juliana Silva da Luz ◽  
Sandro Roberto Valentini ◽  
Paulo Sérgio Cerri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Steroid Receptors ◽  
Primordial Germ Cells ◽  
Spermatogonial Stem Cells ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding

Expression and intracellular localization of Nanos2-homologue protein in primordial germ cells and spermatogonial stem cells

Zygote ◽  
2019 ◽  
Vol 27 (02) ◽  
pp. 82-88 ◽  
Author(s):  
Vivek Pandey ◽  
Anima Tripathi ◽  
Pawan K. Dubey
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Germ Cells ◽  
Expression Patterns ◽  
Intracellular Localization ◽  
Primordial Germ Cells ◽  
Type A ◽  
Spermatogonial Stem Cells ◽  
Single Type ◽  
Rt Pcr

SummaryThe decision by germ cells to differentiate and undergo either oogenesis or spermatogenesis takes place during embryonic development and Nanos plays an important role in this process. The present study was designed to investigate the expression patterns in rat of Nanos2-homologue protein in primordial germ cells (PGCs) over different embryonic developmental days as well as in spermatogonial stem cells (SSCs). Embryos from three different embryonic days (E8.5, E10.5, E11.5) and SSCs were isolated and used to detect Nanos2-homologue protein using immunocytochemistry, western blotting, reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry. Interestingly, Nanos2 expression was detected in PGCs at day E11.5 onwards and up to colonization of PGCs in the genital ridge of fetal gonads. No Nanos2 expression was found in PGCs during early embryonic days (E8.5 and 10.5). Furthermore, immunohistochemical and immunofluorescence data revealed that Nanos2 expression was restricted within a subpopulation of undifferentiated spermatogonia (As, single type A SSCs and Apr, paired type A SSCs). The same results were confirmed by our western blot and RT-PCR data, as Nanos2 protein and transcripts were detected only in PGCs from day E11.5 and in undifferentiated spermatogonia (As and Apr). Furthermore, Nanos2-positive cells were also immunodetected and sorted using flow cytometry from the THY1-positive SSCs population, and this strengthened the idea that these cells are stem cells. Our findings suggested that stage-specific expression of Nanos2 occurred on different embryonic developmental days, while during the postnatal period Nanos2 expression is restricted to As and Apr SSCs.

Growth Response of Adult Germ Cells to Rat Androgen-Binding Protein and Human Sex Hormone-Binding Globulin

1996 ◽  
Vol 45 (3-5) ◽  
pp. 218-221 ◽  
Author(s):  
A. Gérard ◽  
R. Bedjou ◽  
A. Clerc ◽  
F. Maachi ◽  
J. Closset ◽  
...  
Keyword(s):  
Germ Cells ◽  
Growth Response ◽  
Binding Protein ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Androgen Binding Protein ◽  
Androgen Binding

scholarly journals Sex hormone-binding globulin is synthesized in target cells

2002 ◽  
Vol 175 (1) ◽  
pp. 113-120 ◽  
Author(s):  
SM Kahn ◽  
DJ Hryb ◽  
AM Nakhla ◽  
NA Romas ◽  
W Rosner
Keyword(s):  
Steroid Receptors ◽  
Binding Protein ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Estrogen Signaling ◽  
Target Cells ◽  
Hormone Binding ◽  
Multifunctional Protein ◽  
Current State ◽  
Steroid Signaling

Sex hormone-binding globulin (SHBG) is a multifunctional protein that acts in humans to regulate the response to steroids at several junctures. It was originally described as a hepatically secreted protein that is the major binding protein for sex steroids in plasma, thereby regulating the availability of free steroids to hormone-responsive tissues. SHBG also functions as part of a novel steroid-signaling system that is independent of the classical intracellular steroid receptors. Unlike the intracellular steroid receptors that are ligand-activated transcription factors, SHBG mediates androgen and estrogen signaling at the cell membrane by way of cAMP. We have reviewed the current state of knowledge on the SHBG gene and the role of SHBG in steroid signaling (we shall not address its function as a plasma-binding protein).

scholarly journals NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Qisheng Zuo ◽  
Chen Zhang ◽  
Kai Jin ◽  
Jin Jing ◽  
Changhua Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Spermatogonial Stem Cells

Effects of oestradiol on sex hormone binding globulin

1982 ◽  
Vol 101 (2) ◽  
pp. 248-253 ◽  
Author(s):  
Viveca Odlind ◽  
Kerstin Elamsson ◽  
Doris E. Englund ◽  
Arne Victor ◽  
Elof D. B. Johansson
Keyword(s):  
Luteal Phase ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Pronounced Increase ◽  
Menstrual Cycles ◽  
Menopausal Women ◽  
Plasma Oestradiol

Abstract. Sex hormone binding globulin (SHBG) levels were studied for possible effects of oestradiol-17β on SHBG. No change in SHBG plasma was recorded during normal menstrual cycles or during treatment with oestradiol-17β to menopausal women. However, gonadotrophin treatment to amenorrhoeic women to induce ovulation resulted in high oestradiol concentrations and a pronounced increase in SHBG was found during the luteal phase of these cycles. A marked increase of SHBG was also recorded in a woman with pronounced fluctuations of oestradiol during treatment with levonorgestrel sc implants for contraception. In conclusion, effects on SHBG were only found when extraordinarily high levels of plasma oestradiol were recorded.

Sex hormone-binding globulin (SHBG) and androgen levels in women with androgenetic alopecia

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S144 ◽  
Author(s):  
G. SINNECKER ◽  
E. LUDWIG ◽  
A. KRENZ ◽  
R.P. WILLIG
Keyword(s):  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Androgenetic Alopecia ◽  
Hormone Binding ◽  
Androgen Levels
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