Enzymatic Treatment of Whey Proteins in Cow’s Milk Results in Differential Inhibition of IgE-Mediated Mast Cell Activation Compared to T-Cell Activation

2012 ◽  
Vol 159 (3) ◽  
pp. 263-270 ◽  
Author(s):  
Karen Knipping ◽  
Betty C.A.M. van Esch ◽  
Adrie G. van Ieperen-van Dijk ◽  
Els van Hoffen ◽  
Ton van Baalen ◽  
...  
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Whey Proteins ◽  
Enzymatic Treatment ◽  
Mast Cell Activation ◽  
Differential Inhibition ◽  
Ige Mediated

scholarly journals Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis

2013 ◽  
Vol 288 (14) ◽  
pp. 9801-9814 ◽  
Author(s):  
Ivana Hálová ◽  
Lubica Dráberová ◽  
Monika Bambousková ◽  
Martin Machyna ◽  
Lucie Stegurová ◽  
...  
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
T Cell Activation ◽  
Cross Talk ◽  
Cell Activation ◽  
Adaptor Protein ◽  
Mast Cell Activation

Targeting phosphoinositide 3-kinase δ for allergic asthma

2012 ◽  
Vol 40 (1) ◽  
pp. 240-245 ◽  
Author(s):  
Wendy C. Rowan ◽  
Janet L. Smith ◽  
Karen Affleck ◽  
Augustin Amour
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Critical Role ◽  
T Cell Response ◽  
Mast Cell Activation ◽  
Th2 Cytokines ◽  
Onset Of Action ◽  
Phosphoinositide 3 Kinase

Chronic inflammation in the lung has long been linked to the pathogenesis of asthma. Central to this airway inflammation is a T-cell response to allergens, with Th2 cytokines driving the differentiation, survival and function of the major inflammatory cells involved in the allergic cascade. PI3Kδ (phosphoinositide 3-kinase δ) is a lipid kinase, expressed predominantly in leucocytes, where it plays a critical role in immune receptor signalling. A selective PI3Kδ inhibitor is predicted to block T-cell activation in the lung, reducing the production of pro-inflammatory Th2 cytokines. PI3Kδ is also involved in B-cell and mast cell activation. Therefore the inhibition of PI3Kδ should dampen down the inflammatory cascade involved in the asthmatic response through a wide breadth of pharmacology. Current anti-inflammatory therapies, which are based on corticosteroids, are effective in controlling inflammation in mild asthmatics, but moderate/severe asthmatic patients remain poorly controlled, experiencing recurrent exacerbations. Corticosteroids have no effect on mast cell degranulation and do not act directly on B-cells, so, overall, a PI3Kδ inhibitor has the potential to deliver improvements in onset of action, efficacy and reduced exacerbations in moderate/severe asthmatics. Additionally, PI3Kδ inhibition is expected to block effects of Th17 cells, which are increasingly implicated in steroid-insensitive asthma.

scholarly journals The Involvement of Protein Kinase D in T Cell-Induced Mast Cell Activation

2016 ◽  
Vol 171 (3-4) ◽  
pp. 203-208 ◽  
Author(s):  
Pazit Salamon ◽  
Irit Shefler ◽  
Alon Y. Hershko ◽  
Yoseph A. Mekori
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
Protein Kinase ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Protein Kinase D

Identification of the minimal region of peptide derived from ADP-ribosylation factor1 (ARF1) that inhibits IgE-mediated mast cell activation

2019 ◽  
Vol 105 ◽  
pp. 32-37 ◽  
Author(s):  
Ryota Uchida ◽  
Tomonori Egawa ◽  
Yoshio Fujita ◽  
Kazuyuki Furuta ◽  
Hiroaki Taguchi ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Adp Ribosylation ◽  
Ige Mediated ◽  
Minimal Region

scholarly journals MicroRNA-4443 regulates mast cell activation by T cell–derived microvesicles

2018 ◽  
Vol 141 (6) ◽  
pp. 2132-2141.e4 ◽  
Author(s):  
Irit Shefler ◽  
Pazit Salamon ◽  
Francesca Levi-Schaffer ◽  
Adam Mor ◽  
Alon Y. Hershko ◽  
...  
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
Cell Activation ◽  
Mast Cell Activation

scholarly journals Beyond IgE: Alternative Mast Cell Activation Across Different Disease States

2020 ◽  
Vol 21 (4) ◽  
pp. 1498 ◽  
Author(s):  
David O. Lyons ◽  
Nicholas A. Pullen
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Activation ◽  
Autoimmune Disorders ◽  
Receptor Expression ◽  
Mast Cell Activation ◽  
Food Allergies ◽  
Type I ◽  
Disease States ◽  
Ige Mediated

Mast cells are often regarded through the lens of IgE-dependent reactions as a cell specialized only for anti-parasitic and type I hypersensitive responses. However, recently many researchers have begun to appreciate the expansive repertoire of stimuli that mast cells can respond to. After the characterization of the interleukin (IL)-33/suppression of tumorigenicity 2 (ST2) axis of mast cell activation—a pathway that is independent of the adaptive immune system—researchers are revisiting other stimuli to induce mast cell activation and/or subsequent degranulation independent of IgE. This discovery also underscores that mast cells act as important mediators in maintaining body wide homeostasis, especially through barrier defense, and can thus be the source of disease as well. Particularly in the gut, inflammatory bowel diseases (Crohn’s disease, ulcerative colitis, etc.) are characterized with enhanced mast cell activity in the context of autoimmune disease. Mast cells show phenotypic differences based on tissue residency, which could manifest as different receptor expression profiles, allowing for unique mast cell responses (both IgE and non-IgE mediated) across varying tissues as well. This variety in receptor expression suggests mast cells respond differently, such as in the gut where immunosuppressive IL-10 stimulates the development of food allergy or in the lungs where transforming growth factor-β1 (TGF-β1) can enhance mast cell IL-6 production. Such differences in receptor expression illustrate the truly diverse effector capabilities of mast cells, and careful consideration must be given toward the phenotype of mast cells observed in vitro. Given mast cells’ ubiquitous tissue presence and their capability to respond to a broad spectrum of non-IgE stimuli, it is expected that mast cells may also contribute to the progression of autoimmune disorders and other disease states such as metastatic cancer through promoting chronic inflammation in the local tissue microenvironment and ultimately polarizing toward a unique Th17 immune response. Furthermore, these interconnected, atypical activation pathways may crosstalk with IgE-mediated signaling differently across disorders such as parasitism, food allergies, and autoimmune disorders of the gut. In this review, we summarize recent research into familiar and novel pathways of mast cells activation and draw connections to clinical human disease.

scholarly journals Mast Cells Enhance T Cell Activation: Importance of Mast Cell Costimulatory Molecules and Secreted TNF

2006 ◽  
Vol 176 (4) ◽  
pp. 2238-2248 ◽  
Author(s):  
Susumu Nakae ◽  
Hajime Suto ◽  
Motoyasu Iikura ◽  
Maki Kakurai ◽  
Jonathon D. Sedgwick ◽  
...  
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
Mast Cells ◽  
T Cell Activation ◽  
Cell Activation ◽  
Costimulatory Molecules

scholarly journals Alpha7-nicotinic acetylcholine receptors involve the imidacloprid-induced inhibition of IgE-mediated rat and human mast cell activation

RSC Advances ◽  
2017 ◽  
Vol 7 (82) ◽  
pp. 51896-51906 ◽  
Author(s):  
Linbo Shi ◽  
Huaping Xu ◽  
Yujie Wu ◽  
Xin Li ◽  
Li Zou ◽  
...  
Keyword(s):  
Mast Cell ◽  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Human Mast Cell ◽  
Inhibition Mechanism ◽  
Nicotinic Acetylcholine ◽  
Neonicotinoid Insecticide ◽  
Ige Mediated

Although our recent study indicated that imidacloprid, a widely used neonicotinoid insecticide, inhibited IgE-mediated mast cell activation, the inhibition mechanism still remains unclear.

scholarly journals Direct Inhibition of the Allergic Effector Response by Raw Cow’s Milk—An Extensive In Vitro Assessment

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1258
Author(s):  
Suzanne Abbring ◽  
Bart R. J. Blokhuis ◽  
Julie L. Miltenburg ◽  
Kiri G. J. Romano Olmedo ◽  
Johan Garssen ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Activation ◽  
Allergic Diseases ◽  
Raw Milk ◽  
Mast Cell Activation ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Ige Mediated ◽  
Raw Cow’S Milk

The mechanisms underlying the allergy-protective effects of raw cow’s milk are poorly understood. The current focus is mainly on the modulation of T cell responses. In the present study, we investigated whether raw cow’s milk can also directly inhibit mast cells, the key effector cells in IgE-mediated allergic responses. Primary murine bone marrow-derived mast cells (BMMC) and peritoneal mast cells (PMC), were incubated with raw milk, heated raw milk, or shop milk, prior to IgE-mediated activation. The effects on mast cell activation and underlying signaling events were assessed. Raw milk was furthermore fractionated based on molecular size and obtained fractions were tested for their capacity to reduce IgE-mediated mast cell activation. Coincubation of BMMC and PMC with raw milk prior to activation reduced β-hexosaminidase release and IL-6 and IL-13 production, while heated raw milk or shop milk had no effect. The reduced mast cell activation coincided with a reduced intracellular calcium influx. In addition, SYK and ERK phosphorylation levels, both downstream signaling events of the FcεRI, were lower in raw milk-treated BMMC compared to control BMMC, although differences did not reach full significance. Raw milk-treated BMMC furthermore retained membrane-bound IgE expression after allergen stimulation. Raw milk fractionation showed that the heat-sensitive raw milk components responsible for the reduced mast cell activation are likely to have a molecular weight of > 37 kDa. The present study demonstrates that raw cow’s milk can also directly affect mast cell activation. These results extend the current knowledge on mechanisms via which raw cow’s milk prevents allergic diseases, which is crucial for the development of new, microbiologically safe, nutritional strategies to reduce allergic diseases.

scholarly journals The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Kwok Ho Yip ◽  
Natasha Kolesnikoff ◽  
Nicholas Hauschild ◽  
Lisa Biggs ◽  
Angel F. Lopez ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Activation ◽  
Negative Regulator ◽  
Mast Cell Activation ◽  
Ige Mediated
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