scholarly journals Effects of Zinc and DHA on the Epigenetic Regulation of Human Neuronal Cells

2012 ◽  
Vol 29 (1-2) ◽  
pp. 87-98 ◽  
Author(s):  
Nadia Sadli ◽  
M. Leigh Ackland ◽  
Damitha De Mel ◽  
Andrew J. Sinclair ◽  
Cenk Suphioglu
Keyword(s):  
Epigenetic Regulation ◽  
Neuronal Cells ◽  
Human Neuronal Cells

scholarly journals The cytoplasmic tail of the rabies virus G protein is an essential domain controlling death/survival in human neuronal cells

2008 ◽  
Vol 2 (S1) ◽  
Author(s):  
Christophe Prehaud ◽  
Mireille Lafage ◽  
Gene S Tan ◽  
Françoise Mégret ◽  
Pauline Ménager ◽  
...  
Keyword(s):  
G Protein ◽  
Rabies Virus ◽  
Neuronal Cells ◽  
Cytoplasmic Tail ◽  
Essential Domain ◽  
Human Neuronal Cells

The application of magnets directs the orientation of neurite outgrowth in cultured human neuronal cells

2008 ◽  
Vol 174 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Seungchan Kim ◽  
Woo-Seok Im ◽  
Lami Kang ◽  
Soon-Tae Lee ◽  
Kon Chu ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Neuronal Cells ◽  
Human Neuronal Cells

scholarly journals Paired Helical Filament-Forming Region of Tau (297–391) Influences Endogenous Tau Protein and Accumulates in Acidic Compartments in Human Neuronal Cells

2020 ◽  
Vol 432 (17) ◽  
pp. 4891-4907 ◽  
Author(s):  
Saskia J. Pollack ◽  
Jasmine Trigg ◽  
Tahmida Khanom ◽  
Luca Biasetti ◽  
Karen E. Marshall ◽  
...  
Keyword(s):  
Tau Protein ◽  
Neuronal Cells ◽  
Paired Helical Filament ◽  
Human Neuronal Cells ◽  
Acidic Compartments

scholarly journals Insulin Has Multiple Antiamyloidogenic Effects on Human Neuronal Cells

Endocrinology ◽  
2013 ◽  
Vol 154 (1) ◽  
pp. 375-387 ◽  
Author(s):  
Giuseppe Pandini ◽  
Vincenza Pace ◽  
Agata Copani ◽  
Sebastiano Squatrito ◽  
Danilo Milardi ◽  
...  
Keyword(s):  
Glycogen Synthase ◽  
Neuronal Cells ◽  
Neuronal Cell ◽  
Diabetic Patients ◽  
Cell Fractionation ◽  
Intracellular Accumulation ◽  
Insulin Degrading Enzyme ◽  
App Processing ◽  
Gsk 3Β ◽  
Human Neuronal Cells

Alzheimer’s disease is increased in diabetic patients. A defective insulin activity on the brain has been hypothesized to contribute to the neuronal cell dysregulation leading to AD, but the mechanism is not clear. We analyzed the effect of insulin on several molecular steps of amyloid precursor protein (APP) processing and β-amyloid (Aβ) intracellular accumulation in a panel of human neuronal cells and in human embryonic kidney 293 cells overexpressing APP-695. The data indicate that insulin, via its own receptor and the phosphatidylinositol-3-kinase/AKT pathway, influences APP phosphorylation at different sites. This rapid-onset, dose-dependent effect lasts many hours and mainly concerns dephosphorylation at the APP-T668 site. This effect of insulin was confirmed also in a human cortical neuronal cell line and in rat primary neurons. Cell fractionation and immunofluorescence studies indicated that insulin-induced APP-T668 dephosphorylation prevents the translocation of the APP intracellular domain fragment into the nucleus. As a consequence, insulin increases the transcription of antiamyloidogenic proteins such as the insulin-degrading enzyme, involved in Aβ degradation, and α-secretase. In contrast, the transcripts of pro-amyloidogenic proteins such as APP, β-secretase, and glycogen synthase kinase (Gsk)-3β are decreased. Moreover, cell exposure to insulin favors the nonamyloidogenic, α-secretase-dependent APP-processing pathway and reduces Aβ40 and Aβ42 intracellular accumulation, promoting their release in the extracellular compartment. The latter effects of insulin are independent of both Gsk-3β phosphorylation and APP-T668 dephosphorylation, as indicated by experiments with Gsk-3β inhibitors and with cells transfected with the nonphosphorylatable mutated APP-T668A analog. In human neuronal cells, therefore, insulin may prevent Aβ formation and accumulation by multiple mechanisms, both Gsk-3β dependent and independent.

Extracellular release of BACE1 holoproteins from human neuronal cells

2005 ◽  
Vol 338 (2) ◽  
pp. 800-807 ◽  
Author(s):  
Kiyoko S. Murayama ◽  
Fuyuki Kametani ◽  
Wataru Araki
Keyword(s):  
Neuronal Cells ◽  
Extracellular Release ◽  
Human Neuronal Cells

scholarly journals One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays

2016 ◽  
Vol 21 (10) ◽  
pp. 1112-1124 ◽  
Author(s):  
Sheng Dai ◽  
Rong Li ◽  
Yan Long ◽  
Steve Titus ◽  
Jinghua Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
High Throughput ◽  
High Throughput Screening ◽  
Neuronal Cells ◽  
Drug Efficacy ◽  
Human Stem Cells ◽  
Induced Pluripotent Cells ◽  
One Step ◽  
Human Neuronal Cells

Human neuronal cells differentiated from induced pluripotent cells have emerged as a new model system for the study of disease pathophysiology and evaluation of drug efficacy. Differentiated neuronal cells are more similar in genetics and biological content to human brain cells than other animal disease models. However, culture of neuronal cells in assay plates requires a labor-intensive procedure of plate precoating, hampering its applications in high-throughput screening (HTS). We developed a simplified method with one-step seeding of neural stem cells in assay plates by supplementing the medium with a recombinant human vitronectin (VTN), thus avoiding plate precoating. Robust results were obtained from cell viability, calcium response, and neurite outgrowth assays using this new method. Our data demonstrate that this approach greatly simplifies high-throughput assays using neuronal cells differentiated from human stem cells for translational research.

Sign in / Sign up

Export Citation Format

Share Document