Protein Kinase Activity Profiling of Postmortem Human Brain Tissue

2012 ◽  
Vol 10 (1-4) ◽  
pp. 46-48 ◽  
Author(s):  
Jeroen J.M. Hoozemans ◽  
Riet Hilhorst ◽  
Rob Ruijtenbeek ◽  
Annemieke J.M. Rozemuller ◽  
Saskia M. van der Vies
Keyword(s):  
Protein Kinase ◽  
Human Brain ◽  
Brain Tissue ◽  
Kinase Activity ◽  
Protein Kinase Activity ◽  
Human Brain Tissue ◽  
Postmortem Human Brain ◽  
Activity Profiling

Characterization of miRNA Isoform Expression In Schizophrenia Using Postmortem Human Brain Tissue

2019 ◽  
Vol 29 ◽  
pp. S720
Author(s):  
Carrie Wright ◽  
Joo Heon Shin ◽  
Anandita Rajpurohit ◽  
Courtney Williams ◽  
Andrew Jaffe ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Postmortem Human Brain ◽  
Isoform Expression

scholarly journals P2-031: Protein kinase activity profiling on cerebrospinal fluid to find diagnostic biomarkers for Alzheimer's disease

2012 ◽  
Vol 8 (4S_Part_7) ◽  
pp. P274-P274
Author(s):  
Andrea F.N. Rosenberger ◽  
Jeroen Hoozemans ◽  
Riet Hilhorst ◽  
Philip Scheltens ◽  
Wiesje Van der Flier ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cerebrospinal Fluid ◽  
Protein Kinase ◽  
Kinase Activity ◽  
Protein Kinase Activity ◽  
Diagnostic Biomarkers ◽  
Activity Profiling

scholarly journals Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies

2013 ◽  
Vol 453 (1) ◽  
pp. 101-113 ◽  
Author(s):  
Paul Davies ◽  
Kelly M. Hinkle ◽  
Nour N. Sukar ◽  
Bryan Sepulveda ◽  
Roxana Mesias ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Brain ◽  
Brain Tissue ◽  
Kinase Activity ◽  
Expression Patterns ◽  
Significant Proportion ◽  
Leucine Rich Repeat ◽  
Human Brain Tissue ◽  
Robust Detection ◽  
Lrrk2 Protein

Missense mutations in LRRK2 (leucine-rich repeat kinase 2) are a major cause of PD (Parkinson's disease). Several antibodies against LRRK2 have been developed, but results using these polyclonal antibodies have varied widely leading to conflicting conclusions. To address this challenge, the Michael J. Fox Foundation for Parkinson's Research generated a number of monoclonal antibodies targeting epitopes across the LRRK2 protein. In the present paper, we report optimized protocols and results for ten monoclonal antibodies for immunoblotting, immunohistochemistry, immunoprecipitation and kinase activity assays, in rat, mouse and human brain tissue. Several efficacious antibodies were identified, but results demonstrate that the mouse monoclonal N241A/34 is suitable for most applications, with the best overall rabbit monoclonal antibody being c41-2. These antibodies produced a dominant band of the expected size via immunoblotting and a lack of labelling in tissue derived from LRRK2-knockout animals under optimized conditions. A significant proportion of LRRK2 protein localizes to insoluble fractions and no evidence of truncated LRRK2 protein was detected in any fraction from rodent or human tissues. An assay was developed for the robust detection of LRRK2 kinase activity directly from frozen mouse and human brain tissue, but precipitous declines in activity were observed that corresponded to increasing post-mortem intervals and processing times. Finally, we demonstrate the highest levels of brain-localized LRRK2 in the striatum, but note differential expression patterns between rat and mouse in both striatum and cortex. Anti-LRRK2 monoclonal antibodies that are unlimited in availability together with the proposed standardized protocols should aid in the definition of LRRK2 function in both health and disease.

scholarly journals Investigating Aβ plaque development using FTIR micro‐spectroscopy on native postmortem human brain tissue

2020 ◽  
Vol 16 (S2) ◽  
Author(s):  
Dominik Röhr ◽  
Baayla D.C. Boon ◽  
Frederik Großerüschkamp ◽  
Femke Bouwman ◽  
Jeroen J.M. Hoozemans ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue ◽  
Postmortem Human Brain ◽  
Plaque Development

P1-015: PROTEIN KINASE ACTIVITY DECREASES WITH BRAAK STAGE IN HIPPOCAMPAL POSTMORTEM BRAIN TISSUE AS REVEALED BY USING A PEPTIDE-BASED MICROARRAY PLATFORM

2014 ◽  
Vol 10 ◽  
pp. P309-P309
Author(s):  
Andrea Rosenberger ◽  
Riet Hilhorst ◽  
Jeroen Hoozemans ◽  
Wiesje M. van der Flier ◽  
Annemieke Rozemuller ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Brain Tissue ◽  
Kinase Activity ◽  
Microarray Platform ◽  
Postmortem Brain ◽  
Protein Kinase Activity ◽  
Braak Stage ◽  
Postmortem Brain Tissue

scholarly journals P1-294: Protein kinase activity profiling and identification of signalling pathways involved in Alzheimer's disease

2010 ◽  
Vol 6 ◽  
pp. S258-S259
Author(s):  
Saskia M. Van Der Vies ◽  
Riet Hilhorst ◽  
Elise S. Van Haastert ◽  
Rob Ruijtenbeek ◽  
Philip Scheltens ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Protein Kinase ◽  
Kinase Activity ◽  
Signalling Pathways ◽  
Protein Kinase Activity ◽  
Activity Profiling

Cyclic AMP-Dependent Protein Kinase Activity in Human Brain Across Age

1980 ◽  
Vol 35 (1) ◽  
pp. 261-265 ◽  
Author(s):  
M. J. Schmidt ◽  
L. L. Truex ◽  
B. Ghetti ◽  
A. Routtenberg
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Human Brain ◽  
Kinase Activity ◽  
Protein Kinase Activity ◽  
Dependent Protein Kinase ◽  
Dependent Protein

Reactive microglia in aging and dementia: an immunohistochemical study of postmortem human brain tissue

1999 ◽  
Vol 97 (4) ◽  
pp. 383-392 ◽  
Author(s):  
Margit Overmyer ◽  
Seppo Helisalmi ◽  
Hilkka Soininen ◽  
Markku Laakso ◽  
Paavo Riekkinen Sr. ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Immunohistochemical Study ◽  
Human Brain Tissue ◽  
Postmortem Human Brain ◽  
Reactive Microglia
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