Glandular Cells in Vaginal Smears from Posthysterectomy Patients

1997 ◽  
Vol 41 (6) ◽  
pp. 1701-1704 ◽  
Author(s):  
Brent Ponder ◽  
Kevin O. Easley ◽  
Rosa M. Dávila
Author(s):  
Ana Pinto ◽  
Marisa Suárez ◽  
Horácio Scigliano

Total hysterectomy is a very common surgical technique, that removes the cervix and should eliminate the presence of glandular cells in vaginal smears. However, this fact does not always happen. In this study, it is related the case of a 49-year-old woman, hysterectomized 6 years before, which control vaginal smear presents ciliated cylindrical cells without atypia. The cytological diagnosis was the presence of ciliated cylindrical cells, which, with the clinical information provided, were suggestive of possible tubal prolapse. Subsequent histological study confirmed the suspected cytological diagnosis.


1998 ◽  
Vol 42 (6) ◽  
pp. 1403-1408 ◽  
Author(s):  
Rosemary Tambouret ◽  
Martha B. Pitman ◽  
Debra A. Bell

Cytopathology ◽  
2004 ◽  
Vol 15 (4) ◽  
pp. 195-199 ◽  
Author(s):  
F. Eren ◽  
D. Savci ◽  
I. Erbarut ◽  
H. Gokaslan

Author(s):  
Robert W. Mackin

This paper presents two advances towards the automated three-dimensional (3-D) analysis of thick and heavily-overlapped regions in cytological preparations such as cervical/vaginal smears. First, a high speed 3-D brightfield microscope has been developed, allowing the acquisition of image data at speeds approaching 30 optical slices per second. Second, algorithms have been developed to detect and segment nuclei in spite of the extremely high image variability and low contrast typical of such regions. The analysis of such regions is inherently a 3-D problem that cannot be solved reliably with conventional 2-D imaging and image analysis methods.High-Speed 3-D imaging of the specimen is accomplished by moving the specimen axially relative to the objective lens of a standard microscope (Zeiss) at a speed of 30 steps per second, where the stepsize is adjustable from 0.2 - 5μm. The specimen is mounted on a computer-controlled, piezoelectric microstage (Burleigh PZS-100, 68/μm displacement). At each step, an optical slice is acquired using a CCD camera (SONY XC-11/71 IP, Dalsa CA-D1-0256, and CA-D2-0512 have been used) connected to a 4-node array processor system based on the Intel i860 chip.


1961 ◽  
Vol 36 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Béla Flerkó ◽  
Vera Bárdos

ABSTRACT Absence of compensatory ovarian hypertrophy in »constant oestrus rats« from lesions in the anterior hypothalamic area suggests that nervous elements localized in this region play an essential role in the stimulation of gonadotrophin output by diminution of the blood oestrogen level. The constant vaginal oestrus after unilateral ovariectomy in the majority of animals was, however, repeatedly interrupted by vaginal smears of a dioestrous type. The appearance of a dioestrous vaginal smear in the »hypothalamic constant oestrus rats« is often associated with some luteinisation. It is assumed that diminution of the blood oestrogen level by reduction of ovarian tissue in these animals may bring about a release of LH sufficient to cause formation of corpora lutea.


1968 ◽  
Vol 58 (3) ◽  
pp. 364-376 ◽  
Author(s):  
S. Pesonen ◽  
M. Ikonen ◽  
B-J. Procopé ◽  
A. Saure

ABSTRACT The ovaries of ten patients, at least one year after the post-menopause, were incubated with two Δ5-C19-steroids and also studied histochemically. All these patients had post-menopausal uterine bleeding and increased oestrogen excretion of the urine. The urinary estimations of gonadotrophins, 17-KS, 17-OHCS and pregnanediol were carried out on all patients. Vaginal smears were read according to Papanicolaou, and the endometrium and ovaries were studied histologically. The incubation experiments indicate the presence of Δ5-3β-hydroxysteroid-dehydrogenase. When androst-5-ene-3β,17β-diol was used as precursor the formation of testosterone occurred without any concomitant production of DHA and/or androstenedione. This seems to indicate the possible role of the Δ5-pathway in the formation of testosterone by post-menopausal ovarian tissue. The histochemical reactions indicated a reducing activity on NADH, lactate and glucose-6-phosphate, in certain corpora albicantia, atretic follicles and in diffuse thecoma regions in the cortical layer of the ovary. Steroid-3β-ol-dehydrogenase and β-hydroxybutyrate-dehydrogenase were found only at the edges of certain corpora albicantia, in some individual stroma cell groups and in some atretic follicles. Our studies, both biochemical and histochemical, suggest that the observed increase in the urinary oestrogens of the patients studied might in part at least, be of ovarian origin. This opinion is also supported by the postoperative oestrogen values.


1970 ◽  
Vol 63 (4) ◽  
pp. 667-678 ◽  
Author(s):  
Russel J. Reiter

ABSTRACT The influence of early androgen treatment, light deprivation (by blinding), pinealectomy and superior cervical ganglionectomy on the reproductive system of female rats was tested. Early postnatal treatment of rats with testosterone propionate caused adult rats to exhibit the characteristic signs of androgen sterilization; these included polyfollicular ovaries, normal-sized uteri and persistent vaginal cornification. If early androgentreated rats also were blinded the ovaries were smaller in size and contained fewer follicles, the uteri were greatly reduced in size and the incidence of vaginal oestrus was decreased by approximately 50% If in addition to blinding, androgen-sterilized animals were subjected to either removal of the pineal gland or superior cervical ganglia, the reproductive organs and the vaginal smears were indistinguishable from those of testosterone-treated rats with eyes. These data indicate that the inhibitory influence of blinding on the pituitary-ovarian axis was mediated through the sympathetic nervous system and the pineal gland. The restraining influence of light deprivation on the growth of the reproductive organs was not permanent as illustrated by the fact that if these animals were kept to 120 days of age the ovaries and uteri grew to the same level as those of pinealectomized control rats.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 537b-537
Author(s):  
Svoboda V. Pennisi ◽  
Dennis B. McConnell ◽  
Richard W. Henley

Ficus benjamina plants are an integral part of most modern interior landscapes. Reports from growers and interiorscape managers have drawn attention to a specific problem related to large F. benjamina plants, namely the occurrence of a dark oval spot on the abaxial surface of the leaf base. Twelve cultivars of F. benjamina were examined: Christine, Citation, Florida Spire, Kelly, Kiki, Midnight, Monique, Stacey, Wintergreen, Dwarf Nikita, Spearmint, and Starlight. Anatomically, the dorsal gland consisted of one to several layers of densely stained, columnar cells. Positive colorimetric reaction for phenolics was obtained in the glandular cells. Developmentally, the gland cells could not be distinguished from the regular epidermal cells until ≈30% of final leaf size was reached. The cells of the outermost glandular layer changed shape from rectangular with long axis parallel to the leaf surface to elongate with long axis perpendicular to the surface. In a mature leaf, the thickness of the glandular layer was between 20 and 30 μm. Externally, at this stage, no dark spot, indicative of the gland's location, could be observed. In older leaves, however, an accumulation of phenolic substances led to appearance of dorsal dark spot. All cultivars possessed glandular layer. However, this area did not darken in all cultivars; Christine, Citation, Florida Spire, Kelly, Kiki, and Stacey developed small dark spots, while Dwarf Nikita and Starlight had numerous, well-pronounced glandular regions. This study showed that the dark spots in F. benjamina cultivars were a normal morphological feature. Although the gland was present in every cultivar, only a few cultivars developed a dark color.


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