Reassessment of sst5 Somatostatin Receptor Expression in Normal and Neoplastic Human Tissues Using the Novel Rabbit Monoclonal Antibody UMB-4

2011 ◽  
Vol 94 (3) ◽  
pp. 255-264 ◽  
Author(s):  
Amelie Lupp ◽  
Anna Hunder ◽  
Aline Petrich ◽  
Falko Nagel ◽  
Christian Doll ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Somatostatin Receptor ◽  
Receptor Expression ◽  
Human Tissues ◽  
The Novel ◽  
Rabbit Monoclonal Antibody

scholarly journals Reassessment of sst3Somatostatin Receptor Expression in Human Normal and Neoplastic Tissues Using the Novel Rabbit Monoclonal Antibody UMB-5

2012 ◽  
Vol 96 (4) ◽  
pp. 301-310 ◽  
Author(s):  
Amelie Lupp ◽  
Falko Nagel ◽  
Christian Doll ◽  
Christoph Röcken ◽  
Matthias Evert ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Receptor Expression ◽  
The Novel ◽  
Rabbit Monoclonal Antibody ◽  
Neoplastic Tissues

Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8

Peptides ◽  
2015 ◽  
Vol 66 ◽  
pp. 19-25 ◽  
Author(s):  
Amelie Lupp ◽  
Anika Mann ◽  
Alexander Heeb ◽  
Daniel Kaemmerer ◽  
Jörg Sänger ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Tissues ◽  
The Novel ◽  
Endothelin Receptor ◽  
Endothelin Receptor A ◽  
Rabbit Monoclonal Antibody

scholarly journals Reassessment of sst2Somatostatin Receptor Expression in Human Normal and Neoplastic Tissues Using the Novel Rabbit Monoclonal Antibody UMB-1

2008 ◽  
Vol 93 (11) ◽  
pp. 4519-4524 ◽  
Author(s):  
Thomas Fischer ◽  
Christian Doll ◽  
Stefan Jacobs ◽  
Angela Kolodziej ◽  
Ralf Stumm ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Receptor Expression ◽  
The Novel ◽  
Rabbit Monoclonal Antibody ◽  
Neoplastic Tissues

scholarly journals Reassessment of SST4 Somatostatin Receptor Expression Using SST4-eGFP Knockin Mice and the Novel Rabbit Monoclonal Anti-Human SST4 Antibody 7H49L61

2021 ◽  
Vol 22 (23) ◽  
pp. 12981
Author(s):  
Amelie Lupp ◽  
Blanca Ehms ◽  
Ralf Stumm ◽  
Johannes Göckeritz ◽  
Christian Mawrin ◽  
...  
Keyword(s):  
Ganglion Cells ◽  
Somatostatin Receptor ◽  
Somatostatin Receptors ◽  
Broad Band ◽  
Pyramidal Cells ◽  
Receptor Expression ◽  
Human Tissues ◽  
The Novel ◽  
Formalin Fixed Paraffin Embedded ◽  
Egfp Fusion Protein

Among the five somatostatin receptors (SST1–SST5), SST4 is the least characterized, which is in part due to the lack of specific monoclonal antibodies. We generated a knockin mouse model that expresses a carboxyl-terminal SST4-eGFP fusion protein. In addition, we extensively characterized the novel rabbit monoclonal anti-human SST4 antibody 7H49L61 using transfected cells and receptor-expressing tissues. 7H49L61 was then subjected to immunohistochemical staining of a series of formalin-fixed, paraffin-embedded normal and neoplastic human tissues. Characterization of SST4-eGFP mice revealed prominent SST4 expression in cortical pyramidal cells and trigeminal ganglion cells. In the human cortex, 7H49L61 disclosed a virtually identical staining pattern. Specificity of 7H49L61 was demonstrated by detection of a broad band migrating at 50–60 kDa in immunoblots. Tissue immunostaining was abolished by preadsorption of 7H49L61 with its immunizing peptide. In the subsequent immunohistochemical study, 7H49L61 yielded a predominant plasma membrane staining in adrenal cortex, exocrine pancreas, and placenta. SST4 was also found in glioblastomas, parathyroid adenomas, gastric and pancreatic adenocarcinomas, pheochromocytomas, and lymphomas. Altogether, we provide the first unequivocal localization of SST4 in normal and neoplastic human tissues. The monoclonal antibody 7H49L61 may also prove of great value for identifying SST4-expressing tumors during routine histopathological examinations.

Detection of LIM domain only 2 (LMO2) in normal human tissues and haematopoietic and non-haematopoietic tumours using a newly developed rabbit monoclonal antibody

2012 ◽  
Vol 61 (1) ◽  
pp. 33-46 ◽  
Author(s):  
Claudio Agostinelli ◽  
Jennifer C Paterson ◽  
Rajeev Gupta ◽  
Simona Righi ◽  
Federica Sandri ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Tissues ◽  
Lim Domain ◽  
Rabbit Monoclonal Antibody ◽  
Normal Human

scholarly journals EP1: a novel rabbit monoclonal antibody for detection of oestrogen receptor α

2013 ◽  
Vol 66 (12) ◽  
pp. 1051-1057 ◽  
Author(s):  
Sunil Badve ◽  
I Tudor Vladislav ◽  
Betsy Spaulding ◽  
Anna Strickland ◽  
Sylvia Hernandez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Monoclonal Antibody ◽  
Oestrogen Receptor ◽  
Detection System ◽  
Staining Intensity ◽  
Ease Of Use ◽  
Receptor Expression ◽  
Breast Cancer Patients ◽  
Cytoplasmic Staining ◽  
Rabbit Monoclonal Antibody

AimsAssessment of hormone receptor expression is part of routine examination of every breast cancer. In this study, we report the characterisation of a novel rabbit monoclonal antibody, clone EP1, directed against oestrogen receptor (ER) α. Additionally, its immunohistochemical performance characteristics in archival tissues are evaluated in normal tissues and two distinct cohorts of breast cancer patients.MethodsComparative analyses between EP1 and the anti-ERα component of the ER/PR pharmDx kit (cocktail of mouse monoclonal antibody clones 1D5 and ER-2-123) and between EP1 and another commercially available rabbit monoclonal antibody, clone SP1, are described.ResultsClone EP1 specifically detects nuclear ER in all tissues examined; cytoplasmic staining was not observed. The analysis shows a high degree of concordance (∼95%) between EP1 and both the ERα component of the Dako ER/PR pharmDx kit and Ventana clone SP1. However, the use of EP1 antibody together with Dako EnVision FLEX detection system resulted in a stronger staining intensity as compared with SP1 antibody using the Ventana ultraView DAB detection system resulting in better ‘ease of use.’ConclusionsThe use of EPI can result in better interpretation of the results of the ER analysis.

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