Simultaneous Detection of T Lymphocyte-Related Antigens (CD4/CD8, CD57, TCRβ) with Nuclei by Fluorescence-Based Immunohistochemistry in Paraffin-Embedded Human Lymph Node, Liver Cancer and Stomach Cancer

2011 ◽  
Vol 55 (4) ◽  
pp. 357-363 ◽  
Author(s):  
Takahiro Nakata ◽  
Eiji Takayama ◽  
Hirohito Magari ◽  
Jun Kato ◽  
Nobuo Kondo ◽  
...  
Keyword(s):  
Lymph Node ◽  
Liver Cancer ◽  
Stomach Cancer ◽  
T Lymphocyte ◽  
Simultaneous Detection ◽  
Human Lymph Node

BIOPSY OF THE SIGNALING LYMPH NODES IN GASTRIC CANCER - CURRENT STATUS OF THE PROBLEM AND PROSPECTS

2018 ◽  
Vol 64 (3) ◽  
pp. 335-344
Author(s):  
Aleksey Karachun ◽  
Yuriy Pelipas ◽  
Oleg Tkachenko ◽  
D. Asadchaya
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Lymph Nodes ◽  
Stomach Cancer ◽  
Current Status ◽  
Regional Lymph Nodes ◽  
Systematic Analysis ◽  
Tumor Spread ◽  
Special Value

The concept of biopsy of sentinel lymph node as the first lymph node in the pathway of lymphogenous tumor spread has been actively discussed over the past decades and has already taken its rightful place in breast and melanoma surgery. The goal of this method is to exclude vain lymphadenectomy in patients without solid tumor metastases in regional lymph nodes. In the era of minimally invasive and organ-saving operations interventions it seems obvious an idea to introduce a biopsy of sentinel lymph node in surgery of early gastric cancer. Meanwhile the complexity of lymphatic system of the stomach and the presence of so-called skip metastases are factors limiting the introduction of a biopsy of sentinel lymph node in stomach cancer. This article presents a systematic analysis of biopsy technology of signaling lymph node as well as its safety and oncological adequacy. Based on literature data it seems to us that the special value of biopsy of sentinel lymph nodes in the future will be in the selection of personalized surgical tactics for stomach cancer.

scholarly journals Regulatory T lymphocyte infiltration in metastatic breast cancer—an independent prognostic factor that changes with tumor progression

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Jenny Stenström ◽  
Ingrid Hedenfalk ◽  
Catharina Hagerling
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Lymph Node ◽  
Prognostic Factor ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
Metastatic Breast ◽  
Distant Metastases ◽  
Independent Prognostic Factor ◽  
Primary Tumors

Abstract Background Patients diagnosed with metastatic breast cancer have poor outcome with a median survival of approximately 2 years. While novel therapeutic options are urgently needed, the great majority of breast cancer research has focused on the primary tumor and less is known about metastatic breast cancer and the prognostic impact of the metastatic tumor microenvironment. Here we investigate the immune landscape in unique clinical material. We explore how the immune landscape changes with metastatic progression and elucidate the prognostic role of immune cells infiltrating primary tumors and corresponding lymph node and more importantly distant metastases. Methods Immunohistochemical staining was performed on human breast cancer tissue microarrays from primary tumors (n = 231), lymph node metastases (n = 129), and distant metastases (n = 43). Infiltration levels of T lymphocytes (CD3+), regulatory T lymphocytes (Tregs, FOXP3+), macrophages (CD68+), and neutrophils (NE+) were assessed in primary tumors. T lymphocytes and Tregs were further investigated in lymph node and distant metastases. Results T lymphocyte and Treg infiltration were the most clinically important immune cell populations in primary tumors. Infiltration of T lymphocytes and Tregs in primary tumors correlated with proliferation (P = 0.007, P = 0.000) and estrogen receptor negativity (P = 0.046, P = 0.026). While both T lymphocyte and Treg infiltration had a negative correlation to luminal A subtype (P = 0.031, P = 0.000), only Treg infiltration correlated to luminal B (P = 0.034) and triple-negative subtype (P = 0.019). In primary tumors, infiltration of T lymphocytes was an independent prognostic factor for recurrence-free survival (HR = 1.77, CI = 1.01–3.13, P = 0.048), while Treg infiltration was an independent prognostic factor for breast cancer-specific survival (HR = 1.72, CI = 1.14–2.59, P = 0.01). Moreover, breast cancer patients with Treg infiltration in their distant metastases had poor post-recurrence survival (P = 0.039). Treg infiltration levels changed with metastatic tumor progression in 50% of the patients, but there was no significant trend toward neither lower nor higher infiltration. Conclusion Treg infiltration could have clinical applicability as a prognostic biomarker, deciphering metastatic breast cancer patients with worse prognosis, and accordingly, could be a suitable immunotherapeutic target for patients with metastatic breast cancer. Importantly, half of the patients had changes in Treg infiltration during the course of metastatic progression emphasizing the need to characterize the metastatic immune landscape.

scholarly journals Human lymph node morphology as a function of age and site.

1980 ◽  
Vol 33 (5) ◽  
pp. 454-461 ◽  
Author(s):  
P Luscieti ◽  
T Hubschmid ◽  
H Cottier ◽  
M W Hess ◽  
L H Sobin
Keyword(s):  
Lymph Node ◽  
Human Lymph Node

Analysis of lymphoid and dendritic cells in human lymph node, tonsil and spleen

1984 ◽  
Vol 45 (1) ◽  
pp. 169-185 ◽  
Author(s):  
P. Valk ◽  
E. M. Loo ◽  
J. Jansen ◽  
M. R. Daha ◽  
C. J. L. M. Meijer
Keyword(s):  
Dendritic Cells ◽  
Lymph Node ◽  
Human Lymph Node

HUMAN LYMPH-NODE-CELL TRANSFORMATION

The Lancet ◽  
1967 ◽  
Vol 289 (7489) ◽  
pp. 579 ◽  
Author(s):  
C.A Rubio ◽  
J Zajicek
Keyword(s):  
Lymph Node ◽  
Cell Transformation ◽  
Lymph Node Cell ◽  
Human Lymph Node

Visualizing antibody production in a human lymph node in a dish

2021 ◽  
Vol 13 (578) ◽  
pp. eabg5638
Author(s):  
Gerald P. Morris
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Antibody Production ◽  
Antibody Formation ◽  
In Vitro Modeling ◽  
Human Lymph Node

Development of a human lymphoid organoid system enables in vitro modeling of immune responses and antibody formation.

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