Chromosomal Distribution of Repetitive DNA Sequences Highlights the Independent Differentiation of Multiple Sex Chromosomes in Two Closely Related Fish Species

2011 ◽  
Vol 134 (4) ◽  
pp. 295-302 ◽  
Author(s):  
M.B. Cioffi ◽  
W.F. Molina ◽  
O. Moreira-Filho ◽  
L.A.C. Bertollo
Keyword(s):  
Repetitive Dna ◽  
Fish Species ◽  
Sex Chromosomes ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Chromosomal Distribution ◽  
Multiple Sex Chromosomes

Molecular characterization and chromosomal distribution of species-specific repetitive DNA sequences from Beta corolliflora, a wild relative of sugar beet

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1073-1080 ◽  
Author(s):  
D Gao ◽  
T Schmidt ◽  
C Jung
Keyword(s):  
Sugar Beet ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Southern Hybridization ◽  
Repetitive Dna Sequences ◽  
Wild Relative ◽  
Chromosomal Distribution ◽  
Species Specific ◽  
Specific Sequences

Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridization of genomic DNA, six clones were determined to represent highly repetitive DNA families present only in the B. corolliflora genome. Five other sequences were highly repetitive in B. corolliflora and low or single copy in B. vulgaris. The insert size varied between 43 bp and 448 bp. Two sequences pBC1279 and pBC1944 displayed strong homology to a previously cloned satellite DNA from B. nana. With one exception, sequences are tandemly arranged as revealed by a typical ladder pattern after genomic Southern hybridization. The chromosomal distribution of five probes was determined by fluorescence in situ hybridization (FISH) of mitotic metaphases from B. corolliflora and a triploid hybrid between B. vulgaris and B. corolliflora. Three sequences were spread along all chromosome arms of B. corolliflora while one sequence was present on only six chromosomes. The chromosome-specific sequence pBC216 was found in close vicinity to the 5S rDNA located on B. corolliflora chromosome IV. This set of species-specific sequences has the potential to be used as probes for the identification of monosomic alien addition lines and for marker-assisted gene transfer from wild beet to cultivated beet.Key words: Beta vulgaris, FISH, repetitive DNA, species-specific sequences.

Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of Cucumis metuliferus Using Fluorescence in situ Hybridization

2014 ◽  
Vol 144 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Kouhei Yagi ◽  
Ewa Siedlecka ◽  
Magdalena Pawełkowicz ◽  
Michał Wojcieszek ◽  
Zbigniew Przybecki ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Karyotype Analysis ◽  
Repetitive Dna Sequences ◽  
Chromosomal Distribution

scholarly journals Primitive sex chromosomes in poeciliid fishes harbor simple repetitive DNA sequences

1993 ◽  
Vol 265 (3) ◽  
pp. 301-308 ◽  
Author(s):  
Indrajit Nanda ◽  
Manfred Schartl ◽  
Jörg T. Epplen ◽  
Wolfgang Feichtinger ◽  
Michael Schmid
Keyword(s):  
Repetitive Dna ◽  
Sex Chromosomes ◽  
Dna Sequences ◽  
Repetitive Dna Sequences

scholarly journals Repetitive DNA Sequences and Evolution of ZZ/ZW Sex Chromosomes in Characidium (Teleostei: Characiformes)

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0137231 ◽  
Author(s):  
Priscilla Cardim Scacchetti ◽  
Ricardo Utsunomia ◽  
José Carlos Pansonato-Alves ◽  
Guilherme José da Costa Silva ◽  
Marcelo Ricardo Vicari ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Sex Chromosomes ◽  
Dna Sequences ◽  
Repetitive Dna Sequences

Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus

2003 ◽  
Vol 101 (3-4) ◽  
pp. 314-319 ◽  
Author(s):  
S.C. Harvey ◽  
C. Boonphakdee ◽  
R. Campos-Ramos ◽  
M.T. Ezaz ◽  
D.K. Griffin ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Sex Chromosomes ◽  
Dna Sequences ◽  
Oreochromis Niloticus ◽  
Repetitive Dna Sequences
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